The principal characteristics of the investigations performed to specify methods for estimation of the time of death (TD) are presented. The studies included the measurement of electric conductivity of organs and tissues depending on the time of death and the evaluation of contractile activities of smooth muscle cells in response to the application of an electric current. A new method for the estimation of the time of death with the use of morphometric techniques is described. The results of histostereometric measurements are used for the first time to characterize the intensity of autolysis. Forensic medical mycological studies have been carried out. A combined approach to the determination of the time of death is proposed including mathematical simulation.