The aim of the work was to evaluate the effectiveness of rehabilitation after the complete elimination of carious cavities in patients with dentin caries according to clinical and laboratory indicators when using the ELGYDIUM Protection caries toothpaste for cleaning teeth for 6 months. 29 patients with high intensity of dental caries were examined and received treatment. Depending on the organization of daily hygienic measures after treatment the patients were divided into two groups. The main group included 14 patients who used Elgydium Protection caries paste («Pierre Fabre Medicament», France) for brushing their teeth twice a day for 6 months. The control group included 15 patients who used toothpastes with fluoride of the first generation. All patients underwent clinical dental examination with the determination of the DMFT index and the amount of soft dental plaque according to the Silness—Lohe index, as well as in the gingival fluid bacteriological examination of cariogenic microbiota, determination of the proinflammatory cytokine tumor necrosis factor alpha (TNF-α) and an immunoassay for anti-inflammatory peptides α-defensins 1—3. Effective change in the concentration of antimicrobial peptides α-defensins 1—3 and TNF-α after treatment along with successful eradication of cariogenic bacteria from carious cavities, the absence of recurrent dental caries in the main group compared with the control group proved the successful anti-caries action of long-term use of fluorenol capable of forming a uniform film on the surface of the tooth in the presence of silyglycol. The protective film helps to increase the resistance of the tooth to demineralization and prevents plaque formation. Thus, the study results proved the effectiveness of ELGYDIUM Protection caries toothpaste used for 6 months for the reduction of caries.