Clinical experience with noninvasive prenatal diagnosis of fetal rhesus factor

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Ozherelyeva M.A.

Postgraduate Education Department of Obstetrics and Gynecology, Omsk State Medical University, Ministry of Health of Russia, Omsk, Russia

Toropovsky A.N.

OOO “TestGen”, Ulyanovsk, Russia

Kravchenko E.N.

Kafedra akusherstva i ginekologii Tsentra povysheniia kvalifikatsii i poslediplomnoĭ podgotovki spetsialistov Omskoĭ gosudarstvennoĭ meditsinskoĭ akademii

Kuklina L.V.

Kafedra akusherstva i ginekologii fakul'teta poslediplomnogo obrazovaniia GUVPO "Omskaia gosudarstvennaia meditsinskaia akademiia" Minzdrava Rossii

Clinical experience with noninvasive prenatal diagnosis of fetal rhesus factor

Authors:

Ozherelyeva M.A., Toropovsky A.N., Kravchenko E.N., Kuklina L.V.

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Journal: Russian Bulletin of Obstetrician-Gynecologist. 2016;(3): 38‑41

DOI: 10.17116/rosakush201616338-41

Read: 1227 times

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To cite this article:

Ozherelyeva MA, Toropovsky AN, Kravchenko EN, Kuklina LV. Clinical experience with noninvasive prenatal diagnosis of fetal rhesus factor. Russian Bulletin of Obstetrician-Gynecologist. 2016;(3):38‑41. (In Russ.)
https://doi.org/10.17116/rosakush201616338-41

Подписка 2026 Российский вестник акушера-гинеколога (Russian Bulletin of Obstetrician-Gynecologist)

Использование инфографики авторами научных работ

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Abstract:

The paper presents the clinical experience with noninvasive prenatal diagnosis of fetal rhesus (Rh) factor by a pregnant woman’s blood testing. Objective: to define the analytical capabilities of a procedure for the noninvasive determination of fetal Rh factor by a pregnant woman’s blood testing using the reagent kits Test-RHD plus, AmpliPrime DNA-sorb-B, and Rotor-Gene6000. Subjects and methods. The investigation included the results of examinations in 46 Rh-negative pregnant women delivered at the Omsk City Clinical Perinatal Center. Fetal Rh status was diagnosed by the extraction of fetal circulating extracellular DNA from the blood of a pregnant woman and by polymerase chain reaction with hybridization fluorescence detection using a kit of reagents. The findings were used to calculate the analytical capacities of the procedure. Results. Diagnostic accuracy, sensitivity, and specificity were 97.82, 100, and 92.85%, respectively; the prognostic value of positive and negative findings was 96.96 and 100%. Conclusion. The procedure for the noninvasive determination of fetal Rh factor from a pregnant woman’s blood, by applying the reagent kit Test-RHD plus, may be recommended for use in routine laboratory practice by strictly observing the testing conditions. The authors declare no conflicts of interest.

Keywords:

pregnant women; noninvasive prenatal diagnosis

fetal rhesus factor

fetal extracellular DNA

Authors:

Ozherelyeva M.A.

Postgraduate Education Department of Obstetrics and Gynecology, Omsk State Medical University, Ministry of Health of Russia, Omsk, Russia

Toropovsky A.N.

OOO “TestGen”, Ulyanovsk, Russia

Kravchenko E.N.

Kafedra akusherstva i ginekologii Tsentra povysheniia kvalifikatsii i poslediplomnoĭ podgotovki spetsialistov Omskoĭ gosudarstvennoĭ meditsinskoĭ akademii

Kuklina L.V.

Kafedra akusherstva i ginekologii fakul'teta poslediplomnogo obrazovaniia GUVPO "Omskaia gosudarstvennaia meditsinskaia akademiia" Minzdrava Rossii

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References:

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