BACKGROUND

The high prevalence of periodontal disease, with microorganisms being the main etiology, warrants the development of effective antiseptics.

OBJECTIVE

To evaluate the in vitro activity of citrus bioflavonoid complex in combination with chlorhexidine against the periodontopathogenic microorganism P. intermedia.

MATERIALS AND METHODS

Microbial cultures of clinical isolates from the collection of the Department of Microbiology, Immunology, and Virology of A. I. Evdokimov of MSMSU (P. intermedia) were used in the experiment. Primary inoculation of the test material was carried out on growth media produced by Himedia Laboratories Pvt. Limited (India). Basis: Colombia blood agar (M144) with 5 % (v/v) defibrinated blood and a selective additive for non-spore-forming anaerobes isolation. The results were interpreted according to the change in the optical density index (McFarland index) at wavelength λ=850 nm.

RESULTS AND DISCUSSION

The combination of citrus bioflavonoids + chlorhexidine 0.5 % was more effective in suppressing bacterial growth than chlorhexidine 0.05 %, prolonging the adaptive phase (lag phase) of P. intermedia growth. Thus, a rinse based on this combination can be considered as an antiseptic for periodontal disease. Similar results were obtained for the combination of a complex based on citrus bioflavonoids + chlorhexidine 0.2 %.