Biochemical markers of incomplete miscarriage (incomplete spontaneous abortion)

Koshulko P.A.

Ryazan State Medical University named after Acad. I.P. Pavlov

ORCID: 0000-0003-2806-4017

Kovalenko M.S.

Ryazan State Medical University named after Acad. I.P. Pavlov

ResearcherID: T-5410-2017
ORCID: 0000-0002-2275-5426

Abalenikhina Yu.V.

Ryazan State Medical University

Scopus AuthorID: 57195266939
ORCID: 0000-0003-0427-0967

Dmitrieva M.N.

Ryazan State Medical University named after Acad. I.P. Pavlov

ORCID: 0000-0003-0915-026X

Rokunov E.D.

Ryazan State Medical University named after Acad. I.P. Pavlov

ResearcherID: GZG-2568-2022
ORCID: 0000-0002-1817-2439

Biochemical markers of incomplete miscarriage (incomplete spontaneous abortion)

Authors:

Koshulko P.A., Kovalenko M.S., Abalenikhina Yu.V., Dmitrieva M.N., Rokunov E.D.

More about the authors

Journal: Russian Journal of Human Reproduction. 2023;29(5): 79‑84

DOI: 10.17116/repro20232905179

Downloaded: 8

Download PDF (RU)

Contact the author

Table of contents

To cite this article:

Koshulko PA, Kovalenko MS, Abalenikhina YuV, Dmitrieva MN, Rokunov ED. Biochemical markers of incomplete miscarriage (incomplete spontaneous abortion). Russian Journal of Human Reproduction. 2023;29(5):79‑84. (In Russ.)
https://doi.org/10.17116/repro20232905179

Подписка 2025-2 (Russian Journal of Human Reproduction)

Close metadata

PURPOSE OF THE STUDY

Creation and implementation in practice of a methodology for early diagnosis and prediction of pregnancy pathology (incomplete miscarriage) based on changes in the level of activity of blood proteases.

MATERIAL AND METHODS

The study included 70 pregnant women, who made up two groups: the 1st (control group) was represented by 35 clinically healthy patients with a physiologically ongoing pregnancy. And the second (main) group included the remaining 35 patients with incomplete spontaneous abortion. The activity of proteases was studied using kits for determining the activity of cathepsins, with its further assessment by spectrofluoriometric methods.

RESULTS

The study proved the high specificity and sensitivity of new methods for diagnosing incomplete miscarriage.

CONCLUSION

According to the study, the role of cathepsins as markers of incomplete spontaneous abortion was proved and a method for early diagnosis and prediction of this pathology of pregnancy was created.

Keywords:

cathepsins B

proteases

anembryony

incomplete spontaneous abortion

miscarriage

incomplete miscarriage

Authors:

Koshulko P.A.

Ryazan State Medical University named after Acad. I.P. Pavlov

ORCID: 0000-0003-2806-4017

Kovalenko M.S.

Ryazan State Medical University named after Acad. I.P. Pavlov

ResearcherID: T-5410-2017
ORCID: 0000-0002-2275-5426

Abalenikhina Yu.V.

Ryazan State Medical University

Scopus AuthorID: 57195266939
ORCID: 0000-0003-0427-0967

Dmitrieva M.N.

Ryazan State Medical University named after Acad. I.P. Pavlov

ORCID: 0000-0003-0915-026X

Rokunov E.D.

Ryazan State Medical University named after Acad. I.P. Pavlov

ResearcherID: GZG-2568-2022
ORCID: 0000-0002-1817-2439

Received:

18.10.2022

Accepted:

16.08.2023

List of references:

Wyatt PR, Owolabi T, Meier C, Huang T. Age-specific risk of fetal loss observed in a second trimester serum screening population. Obstetrics & Gynecology. 2005;192(6):231-237. https://doi.org/10.1016/j.ajog.2004.06.099
Vaskoska R, Ha M, Ong L, Kearney G, White JD, Gras S, Warner RD. Ageing and cathepsin inhibition affect the shrinkage of fibre fragments of bovine semitendinosus, biceps femoris and psoas major during heating. Meat Science. 2021;172(7):37-45. https://doi.org/10.1016/j.meatsci.2020.108339
Andrault PM, Panwar P, Brömme D. Characterization of cathepsin S exosites that govern its elastolytic activity. Biochemical Journal. 2020;477(1):227-242. https://doi.org/10.1042/BCJ20190847
Di Spiezio A, Marques ARA, Schmidt L, Thießen N, Gallwitz L, Fogh J, Bartsch U, Saftig P. Analysis of cathepsin B and cathepsin L treatment to clear toxic lysosomal protein aggregates in neuronal ceroid lipofuscinosis. Biochimica et Biophysica Acta. 2021;1867(10): 47-55. https://doi.org/10.1016/j.bbadis.2021.166205
Lu J, Wang M, Wang Z, Fu Z, Lu A, Zhang G. Advances in the discovery of cathepsin K inhibitors on bone resorption. Journal of Enzyme Inhibition and Medicinal Chemistry. 2018;33(1):890-904. https://doi.org/10.1080/14756366.2018.1465417
Akinyemi AO, Pereira GB, Rocha FV. Role of cathepsin B in cancer progression: A potential target for coordination compounds. Mini-Reviews in Medicinal Chemistry. 2021;21(13):1612-1624. https://doi.org/10.2174/1389557521666210212152937
Vitzthum VJ, Spielvogel H, Thornburg J, West B. A prospective study of early pregnancy loss in humans. Fertility and Sterility. 2006;86(4):355-361. https://doi.org/10.1016/j.fertnstert.2006.01.021
Sawaya GF, Grady D, Kerlikowske K, Grimes DA. Antibiotics at the time of induced abortion: the case for universal prophylaxis based on a meta-analysis. Obstetrics & Gynecology. 1996;87(5 Pt 2):884-890.
Bick RL. Recurrent miscarriage syndrome and infertility caused by blood coagulation protein or platelet defects. Hematologic Clinical. 2000;14(5):1117-31.
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Biochemistry. 1976;7(72):248-254. https://doi.org/10.1006/abio.1976.9999
Brugiere O, Verleden SE. Putting the spotlight on macrophage-derived cathepsin in the pathophysiology of obliterative bronchiolitis. European Respiratory Journal. 2021;57(5): 2004607. https://doi.org/10.1183/13993003.04607-2020
Stavreus-Evers A, Aghajanova L, Brismar H, Eriksson H, Landgren B-M, Hovatta O. Co-existence of he-parin-binding epidermal growth factor-like growth factor and pinopodes in human endometrium at the time of implantation. Molecular Human Reproduction. 2002;8(8):765-770. https://doi.org/10.1093/molehr/8.8.765
Xu Q, Mariman ECM, Goossens GH, Blaak EE, Jocken JWE. Cathepsin gene expression in abdominal subcutaneous adipose tissue of obese/overweight humans. Adipocyte. 2020;9(1):246-252. https://doi.org/10.1080/21623945.2020.1775035
Sato T, Yamashina S, Izumi K, Ueno T, Koike M, Ikejima K, Peters C, Watanabe S. Cathepsin L-deficiency enhances liver regeneration after partial hepatectomy. Life Sciences. 2019;221(15):293-300. https://doi.org/10.1016/j.lfs.2019.02.040
Zhou Z, Chen X, Zhu M, Wang W, Ao Z, Zhao J, Tang W, Hong L. Cathepsin D knockdown regulates biological behaviors of granulosa cells and affects litter size traits in goats. Journal of Zhejiang University Science B. 2021;22(11):893-905. https://doi.org/10.1631/jzus.B2100366
Nagakannan P, Islam MI, Conrad M, Eftekharpour E. Cathepsin B is an executioner of ferroptosis. Eftekharpour. Biochimica et Biophysica Acta. 2021;1868(3):118928. https://doi.org/10.1016/j.bbamcr.2020.118928
Park S, Huang H, Kwon W, Kim H-Y, Park J-K, Han JE, Cho G-J, Han S-H, Sung Y, Ryoo ZY, Kim MO, Choi S-K. Cathepsin A regulates pluripotency, proliferation and differentiation in mouse embryonic stem cells. Cell Biochemistry & Function. 2021;39(1):67-76. https://doi.org/10.1002/cbf.3554
Bhuiyan AI, Rathod P, Ghoshal S, Dana D, Das T, Li G, Dickson AA, Rafi F, Subramaniam GS, Fath KR, Paroly S, Chang EJ, Pathak SK. Clickable, selective, and cell-permeable activity-based probe of human cathepsin B — Minimalistic approach for enhanced selectivity. Bioorganic Chemistry. 2021;117(3):21-29. https://doi.org/10.1016/j.bioorg.2021.105463
Choudhury D, Biswas S. Structure-guided protein engineering of human cathepsin L for efficient collagenolytic activity. Protein Engineering, Design and Selection. 2021;34(4):36-43. https://doi.org/10.1093/protein/gzab005
Morrone C, Smirnova NF, Jeridi A, Kneidinger N, Hollauer C, Schupp JC, Kaminski N, Jenne D, Eickelberg O, Yildirim AÖ. Cathepsin B promotes collagen biosynthesis, which drives bronchiolitis obliterans syndrome. European Respiratory Journal. 2021;57(5): 37-45. https://doi.org/10.1183/13993003.01416-2020
Pontious C, Kaul S, Hong M, Hart PA, Krishna SG, Lara L, Conwell DL, Cruz-Monserrate Z. Cathepsin E expression and activity: Role in the detection and treatment of pancreatic cancer. Pancreatology. 2019;19(7):951-956. https://doi.org/10.1016/j.pan.2019.09.009
Karimkhanloo H, Keenan SN, Sun EW, Wattchow DA, Keating DJ, Montgomery MK, Watt MJ. Circulating cathepsin S improves glycaemic control in mice. Journal of Endocrinology. 2021;248(2):167-179. https://doi.org/10.1530/JOE-20-0408
Brown R, Nath S, Lora A, Samaha G, Elgamal Z, Kaiser R, Taggart C, Weldon S, Geraghty P. Cathepsin S: investigating an old player in lung disease pathogenesis, comorbidities, and potential therapeutics. Respiratory Research. 2020;21(111):71-82.

Close metadata