Objective — to study the advantages of using an extended panel of STS-markers in diagnosing deletions of the AZFY chromosome region. Material and methods. 205 males from infertile couples were included in research. According to the analysis of the ejaculate, which was conducted in appliance with the recommendations of WHO 2010, two groups were identified: 143 patients with azoospermia and oligozoospermia (concentration less than 5 million in 1 ml) comprised the experimental group and 62 patients with normozoospermia comprised control group. Real-time PCR were provided to all males to identify AZF deletions by the presence of the following STS markers: AZFa: sY86, sY84, sY615; AZFb: sY127, sY134, sY142 AZFc: sY242, sY254, sY1291, sY255, sY1192, sY1197, sY1206, sY1125. Results. In experimental group deletions of AZF were detected: sY127, sY134, sY142, sY242, sY254, sY1291, sY255, sY1192, sY1197, sY1206, sY1125 in 32 (22%) males. The highest frequency of deletions in the oligozoospermic group was observed for 2 markers: sY1192 — 20.98%, at besides this deletion was isolated in 11% of cases. sY1291 was detected in 9.79% cases, but this deletion was isolated only in one patient. Isolated deletions of these regions are also found in patients with normozoospermia: sY1192 — 8.06%, sY1291 — 3.23%. The revealed differences between the groups do not reach statistical validity. Conclusion. Deletions of markers sY1192 and sY1291 were found in both groups with disturbed spermatogenesis and in the group with normozoospermia. Moreover, the deletion of sY1192 is isolated in both groups with an almost identical frequency of 11 and 8.06%, accordingly. In this regard clinical significance of the isolated deletion of the sY1192 marker seems questionable. sY1291 deletion was detected 3 times more often in the group of men with impaired spermatogenesis.