Human papillomavirus genotype 16 reproduction and viral DNA integration in the altered cervical epithelium

Ershov V.A.; Viazovaia A.A.; Il'inskaia E.V.; Lisianskaia A.S.; Kuevda D.A.; Trofimova O.B.; Shipulina O.Iu.; Narvskaia O.V.

doi:https://doi.org/

Ershov V.A.

SPb GUZ "Gorodskoĭ klinicheskiĭ onkologicheskiĭ dispanser"

Viazovaia A.A.

FGUN "Sankt-Peterburgskiĭ nauchno-issledovatel'skiĭ institut épidemiologii i mikrobiologii im. Pastera" Rospotrebnadzora

Il'inskaia E.V.

City Hospital #1, Novokuznetsk

Lisianskaia A.S.

Sankt-Peterburgskiĭ gosudarstvennyĭ meditsinskiĭ universitet im. I.P. Pavlova

Kuevda D.A.

FBUN "Tsentral'nyĭ nauchno-issledovatel'skiĭ institut épidemiologii" Rospotrebnadzora, Moskva

Trofimova O.B.

FGUN "Tsentral'nyĭ nauchno-issledovatel'skiĭ institut épidemiologii" Rospotrebnadzora, Moskva

Shipulina O.Iu.

TsNII épidemiologii Rospotrebnadzora

Narvskaia O.V.

FGUN "Sankt-Peterburgskiĭ nauchno-issledovatel'skiĭ institut épidemiologii i mikrobiologii im. Pastera" Rospotrebnadzora

Human papillomavirus genotype 16 reproduction and viral DNA integration in the altered cervical epithelium

Authors:

Ershov V.A., Viazovaia A.A., Il'inskaia E.V., Lisianskaia A.S., Kuevda D.A., Trofimova O.B., Shipulina O.Iu., Narvskaia O.V.

More about the authors

Journal: P.A. Herzen Journal of Oncology. 2013;2(1): 21‑26

Read: 3049 times

Download PDF (RU)

Contact the author

Table of contents

To cite this article:

Ershov VA, Viazovaia AA, Il'inskaia EV, et al. Human papillomavirus genotype 16 reproduction and viral DNA integration in the altered cervical epithelium. P.A. Herzen Journal of Oncology. 2013;2(1):21‑26. (In Russ.)

Подписка 2026 Онкология. Журнал им. П.А. Герцена (P.A. Herzen Journal of Oncology)

Использование инфографики авторами научных работ

Close metadata

Recommended articles:

Isoforms of glial cell line-derived neurotrophic factor and their therapeutic potential. Burdenko's Journal of Neurosurgery. 2025;(6-2):38-43

Role of lipopolysaccharide in the development of inflammation in placental tissue in preeclampsia. Russian Journal of Human Reproduction. 2026;(2):97-103

Abstract:

Fifty-seven from human papillomavirus (HPV)-positive cervical epithelial neoplasias and their adjacent sites were studied using cytology, histology, immunomorphology, electron microscopy, and polymerase chain reaction. The occurrence of episomal and low-grade integrated forms of HPV 16 DNA and the expression of its L1 capsid protein decreased with the higher grade of cervical epithelial lesion. More than 33% integration of HPV 16 DNA was found only in cases of high-grade intraepithelial squamous epithelial lesion (HISEL) and squamous cell carcinoma (SCC). HPV 16 L1 capsid protein production by atypical cells was detected in 2.3% of HISEL cases. L1 protein was produced by the cells of the squamous and metaplastic epithelium adjacent to the CIN sites and in the paratumoral zone in the other cases of HISEL and in cases of SCC.

Keywords:

HPV 16 L1 capsid protein

expression

cervix uteri

Authors:

Ershov V.A.

SPb GUZ "Gorodskoĭ klinicheskiĭ onkologicheskiĭ dispanser"

Viazovaia A.A.

FGUN "Sankt-Peterburgskiĭ nauchno-issledovatel'skiĭ institut épidemiologii i mikrobiologii im. Pastera" Rospotrebnadzora

Il'inskaia E.V.

City Hospital #1, Novokuznetsk

Lisianskaia A.S.

Sankt-Peterburgskiĭ gosudarstvennyĭ meditsinskiĭ universitet im. I.P. Pavlova

Kuevda D.A.

FBUN "Tsentral'nyĭ nauchno-issledovatel'skiĭ institut épidemiologii" Rospotrebnadzora, Moskva

Trofimova O.B.

FGUN "Tsentral'nyĭ nauchno-issledovatel'skiĭ institut épidemiologii" Rospotrebnadzora, Moskva

Shipulina O.Iu.

TsNII épidemiologii Rospotrebnadzora

Narvskaia O.V.

FGUN "Sankt-Peterburgskiĭ nauchno-issledovatel'skiĭ institut épidemiologii i mikrobiologii im. Pastera" Rospotrebnadzora

Close metadata

References:

Mole S., McFarlane M., Chuen-Im T., Milligan S.G., Millan D., Graham S.V. RNA splicing factors regulated by HPV16 during cervical tumour progression. J. Pathol. 2009; 219 (3): 383-91.
Ramírez-Salazar E., Centeno F., Nieto K., Valencia-Hernández A., Salcedo M., Garrido E. HPV16 E2 could act as down-regulator in cellular genes implicated in apoptosis,proliferation and cell differentiation. Virol. J. 2011; 20 (8): 247.
Xue Y., Bellanger S., Zhang W., Lim D., Low J., Lunny D., Thierry F. HPV16 E2 is an immediate early marker of viral infection, preceding E7 expression in precursor structures of cervical carcinoma. Cancer Res. 2010; 70 (13): 5316-25.
Pyeon D., Pearce S.M., Lank S.M., Ahlquist P., Lambert P.F. Establishment of human papillomavirus infection requires cell cycle progression. PloS. Pathog. 2009; 5 (2): e1000318.
Vyazovaya A.A., Kuevda D.A., Trofimova O.B., Ershov V.A., Narvskaya O.V. Integratsiya DNK virusa papillomy cheloveka 16 genotipa v genom kletok tservikal'nogo epiteliya pri fonovykh protsessakh i neoplazii sheiki matki. V kn.: Papillomavirusnaya infektsiya i rak. Integrirovannaya sistema nadzora i profilaktiki. Materialy II simpoziuma s mezhdunarodnym uchastiem. SPb.: NIIEM im. Pastera; 2011: 12-4.
Kiseleva V.I., Krikunova L.I., Lyubina L.V., Bezyaeva G.P., Panarina L.V., Kuevda D.A. i dr. Integratsiya DNK VPCh-16 v kletochnyi genom i prognoz raka sheiki matki. V kn.: Molekulyarnaya diagnostika - 2010: Sbornik trudov VII Vserossiiskoi nauchno-prakticheskoi konferentsii s mezhdunarodnym uchastiem. M.; 2010; t. 3: 371-3.
Badaracco G., Venuti A. Physical status of HPV types 16 and 18 in topographically different areas of genital tumours and in paired tumour-free mucosa. Int. J. Oncol. 2005; 27 (1): 161-7.
Doorbar J. The papillomavirus life cycle. J. Clin. Virol. 2005; 32 (Suppl. 1): 7-15.
Griesser H., Sander H., Walczak C., Hilfrich R.A. HPV vaccine protein L1 predicts disease outcome of high-risk HPV+ early squamous dysplastic lesions. Am. J. Clin. Pathol. 2009; 132 (6): 840-5.
Stemberger-Papić S., Vrdoljak-Mozetic D., Ostojić D.V., Rubesa-Mihaliyevic R., Manestar M. Evaluation of the HPV L1 capsid protein in prognosis of mild and moderate dysplasia of the cervix uteri. Coll. Anthropol. 2010; 34 (2): 419-23.
Kösel S., Burggraf S., Engelhardt W., Olgemöller B. Correlation of HPV16 L1 capsid protein expression in cervical dysplasia with HPV16 DNA concentration, HPV16 E6*I mRNA and histologic outcome. Acta Cytol. 2009; 53 (4): 396-401.
Apgar B.S., Zoschnick L., Wright T.C. Jr. The 2001 Bethesda system terminology. Am. Fam. Physician. 2003; 68 (15): 1992-8.
In: Tavassoli F.A., Devilee P., eds. Pathology and genetics of tumours of the breast and female genital organs. WHO classification of tumours. Lyon: IARC Press; 2003. 432 p.
Kuevda D.A., Ermakova N.V., Shipulina O.Yu., Minkina G.N., Kiseleva V.I., Shinkarkina A.P. Vysokaya virusnaya nagruzka i integratsiya VPCh v genom cheloveka kak molekulyarnye markery displasticheskikh izmenenii sheiki matki. V kn.: Pokrovskii V.I., red. Genodiagnostika infektsionnykh boleznei - 2007: Sbornik trudov. M.: Universitetskaya kniga; Pais; 2007; t. 3: 430.
Andersson S., Safari H., Mints M., Lewensohn-Fuchs I., Gyllensten U., Johansson B. Type distribution, viral load and integration status of high-risk human papillomaviruses in pre-stages of cervical cancer (CIN). Br. J. Cancer. 2005; 92: 2195-200.
Jiang M., Baseman J., Koutsky L., Feng Q., Mao C., Kiviat N., Fu L. Sequence variation of human papillomavirus type 16 and measurement of viral integration by quantitative PCR. J. Clin. Microbiol. 2009; 47 (3): 521-6.
McMurray H.R., Nguyen D., Westbrook T.F., Mcance D.J. Biology of human papillomavirus. Int. J. Pathol. 2001; 82: 13-33.
Cumming S.A., Cheun-Im T., Milligan S.G., Graham S.V. Human papillomavirus type 16 late gene expression is regulated by cellular RNA processing factors in response to epithelial differentiation. Biochem. Soc. Trans. 2008; 36 (3): 522-4.
Kukimoto I., Mori S., Sato H., Takeuchi T., Kanda T. Transcription factor human Skn-1a enhances replication of human papillomavirus DNA through the direct binding to two sites near the viral replication origin. FEBS J. 2008; 275 (12): 3123-35.
Ungureanu C., Socolov D., Anton G., Mihailovici M.S., Teleman S. Immunocytochemical expression of p16INK4a and HPV L1 capsid proteins as predictive markers of the cervical lesions progression risk. Rom. J. Morphol. Embryol. 2010; 51 (3): 497-503.
Karageosov I., Dimova R., Makaveeva V. Electron microscopic detection of viruses in cervix papilloma. Zentralbl. Gynäkol. 1985; 107 (3): 187-91.
Multhaupt H.A., Rafferty P.A., Warhol M.J. Ultrastructural localization of human papilloma virus by nonradioactive in situ hybridization on tissue of human cervical intraepithelial neoplasia. Lab. Invest. 1992; 67 (4): 512-8.
Sato S., Chiba H., Shikano K. Ultrastructural observationof human papillomavirus particles in the uterine cervix intraepithelial neoplasia. Gan No Rinsho. 1988; 34 (8): 993-1000.
Wang H.-K., Duffy A.A., Broker T.R., Chow L.T. Robust production and passaging of infectious HPV in sguamous epithelium of primary human keratinocytes. Genes Dev. 2009; 23: 181-94.
Ershov V.A., Narvskaya O.V. Fonovye protsessy i neoplaziya epiteliya sheiki matki. SPb.: Chelovek; 2007. 80 s.
Badaracco G., Venuti A., Sedati A., Mareante M.L. HPV 16 and HPV 18 in genital tumors: significantly different levels of viral integration and correlation to tumor invasiveness. J. Med. Virol. 2002; 67: 574-82.
Pirami L., Giache V., Becciolini A. Analysis of HPV 16, 18, 31 and 35 DNA in preinvasive and invasive lesions of the uterine cervix. J. Clin. Pathol. 1997; 50: 600-4.