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Биоинформатический подбор наиболее оптимальных комбинаций эндонуклеаз рестрикции для генотипирования важнейших сельскохозяйственных растений методом GBS

Биоинформатический подбор наиболее оптимальных комбинаций эндонуклеаз рестрикции для генотипирования важнейших сельскохозяйственных растений методом GBS

Авторы:
Ерофеева Т.В.,
Крылова А.С.,
Тощаков С.В.

Журнал: Молекулярная генетика, микробиология и вирусология. 2025;43(4-2):157-162.

DOI: 10.17116/molgen202543042157

Прочитано: 627 раз

Резюме

ЦЕЛЬ ИССЛЕДОВАНИЯ

Анализ эндонуклеаз рестрикции для проведения полногеномной рестрикции in silico с помощью программы GBSX для дальнейшего использования в генотипировании секвенированием.

МАТЕРИАЛ И МЕТОДЫ

В исследование было включено 7 геномов сельскохозяйственных культур, таких как рожь, ячмень, просо, овес, кукуруза, гречиха, соя. По литературным данным были подобраны 3 одиночные рестриктазы и 12 комбинаций эндонуклеаз, которые активно используются в мировой практике. Проведена полногеномная рестрикция in silico с помощью программы GBSX каждого исследуемого растения, отобраны и подсчитаны фрагменты нужной длины для дальнейшего высокопроизводительного секвенирования.

РЕЗУЛЬТАТЫ

По результатам исследования была показана возможность использования широкого спектра эндонуклеаз рестрикции для проведения генотипирования секвенированием изучаемых видов сельскохозяйственных растений, а также предложены варианты наиболее перспективных ферментов и их комбинаций, среди которых MseI, ApekI и комбинации: MseI-HaeIII и BfuCI-NspI.

ЗАКЛЮЧЕНИЕ

Все исследуемые эндонуклеазы рестрикции представлены на российском рынке и у всех, за исключением метил-чувствительной эндонуклеазы ApeKI, есть отечественные аналоги. Результаты работы могут быть использованы для решения задач геномной селекции с применением метода GBS (Genotyping by Sequencing, генотипирование секвенированием).

Ключевые слова

  • GBS
  • in silico рестрикция
  • эндонуклеазы рестрикции
  • сельскохозяйственные растения
  • генотипирование
  • селекция

Дата поступления: 11.09.2025

Дата принятия в печать: 15.10.2025

Дата публикации: 08.12.2025

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