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Karelina K.V.

State Scientific Center of Virology and Biotechnology “Vector”

Protopopova E.V.

State Scientific Center of Virology and Biotechnology “Vector”

Pashkovskii S.E.

State Scientific Center of Virology and Biotechnology “Vector”

Ternovoi V.A.

State Scientific Center of Virology and Biotechnology “Vector”

Bayandin R.B.

State Scientific Center of Virology and Biotechnology “Vector”

Production and characterization in vitro of recombinant RCL domain of serpin Ipis of Ixodes persulcatus ticks

Authors:

Karelina K.V., Protopopova E.V., Pashkovskii S.E., Ternovoi V.A., Bayandin R.B.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2025;43(3): 25‑30

DOI: 10.17116/molgen20254303125

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Table of contents

PRODUCTION AND CHARACTERIZATION IN VITRO OF RECOMBINANT RCL DOMAIN OF SERPIN IPIS OF IXODES PERSULCATUS TICKS
PRODUCTION AND CHARACTERIZATION IN VITRO OF RECOMBINANT RCL DOMAIN OF SERPIN IPIS OF IXODES PERSULCATUS TICKS

To cite this article:

Karelina KV, Protopopova EV, Pashkovskii SE, Ternovoi VA, Bayandin RB. Production and characterization in vitro of recombinant RCL domain of serpin Ipis of Ixodes persulcatus ticks. Molecular Genetics, Microbiology and Virology. 2025;43(3):25‑30. (In Russ.)
https://doi.org/10.17116/molgen20254303125

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
Abstract:

Ixodes ticks are vectors of many infectious human and animal pathogens. During the biting process, the tick secretes specific proteins produced in its salivary glands. These proteins play key roles in various biological processes, including modulation of the host immune response, pathogen transmission, blood processing, and tick reproduction and development. Among these proteins, serpins have received special attention and are considered as promising candidates for the development of vaccines and methods to control infectious diseases transmitted by ticks.

Research aim. To obtain and characterize in vitro recombinant RCL domain of serpin Ipis of ticks Ixodes persulcatus.

MATERIAL AND METHODS

PCR product was cloned into PCR 2.1 and pET-200 D/TOPO plasmids. Transfection was performed on chemically competent E. coli One Shot TOP10 and E. coli BL21(DE3) cells. The protein was purified from impurities by nickel chelate chromatography using Ni-NTA-Superflow resin. To analyze the immunogenicity of the obtained protein, mice of BALB/c line were immunized. The obtained sera of laboratory animals were tested by enzyme immunoassay. The structures of tick serpins were constructed by homology modeling using AlphaFold server (https://www.alphafold.com/).

RESULTS

The recombinant RCL domain of serpin RCL of Ipis serpin - ticks Ixodes persulcatus was obtained and characterized in this study. The immunogenicity of the recombinant protein was evaluated by immunization of laboratory animals and the specific activity of the obtained sera was assessed by ELISA and immunoblot methods. 3D models of tick serpins were constructed.

CONCLUSION

Thus, in this study, the recombinant RCL domain of serpin Ipis of Ixodes persulcatus ticks was obtained and characterized. After additional studies, the obtained recombinant RCL domain of serpin Ipis is planned to be used as mono- and multi-antigenic protein vaccines.

Keywords:

recombinant protein
Ipis serpin
plasmid
tick Ixodes persulcatus
RCL-domain

Authors:

Karelina K.V.

State Scientific Center of Virology and Biotechnology “Vector”

Protopopova E.V.

State Scientific Center of Virology and Biotechnology “Vector”

Pashkovskii S.E.

State Scientific Center of Virology and Biotechnology “Vector”

Ternovoi V.A.

State Scientific Center of Virology and Biotechnology “Vector”

Bayandin R.B.

State Scientific Center of Virology and Biotechnology “Vector”

Received:

03.03.2025

Accepted:

31.05.2025

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