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Tolordava E.R.

P.N. Lebedev Physical Institute of RAS;
N.F. Gamaleya National Research Center

Nastulyavichus A.A.

P.N. Lebedev Physical Institute of RAS

Ulturgasheva E.V.

P.N. Lebedev Physical Institute of RAS

Shelygina S.N.

P.N. Lebedev Physical Institute of RAS

Saraeva I.N.

P.N. Lebedev Physical Institute of RAS

Kudryashov S.I.

P.N. Lebedev Physical Institute of RAS

Antibacterial activity of metal nanoparticles in ex vivo wound infection model

Authors:

Tolordava E.R., Nastulyavichus A.A., Ulturgasheva E.V., Shelygina S.N., Saraeva I.N., Kudryashov S.I.

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Journal: Molecular Genetics, Microbiology and Virology. 2024;42(3): 37‑42

DOI: 10.17116/molgen20244203137

Read: 488 times

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Table of contents

ANTIBACTERIAL ACTIVITY OF METAL NANOPARTICLES IN EX VIVO WOUND INFECTION MODEL
ANTIBACTERIAL ACTIVITY OF METAL NANOPARTICLES IN EX VIVO WOUND INFECTION MODEL

To cite this article:

Tolordava ER, Nastulyavichus AA, Ulturgasheva EV, Shelygina SN, Saraeva IN, Kudryashov SI. Antibacterial activity of metal nanoparticles in ex vivo wound infection model. Molecular Genetics, Microbiology and Virology. 2024;42(3):37‑42. (In Russ.)
https://doi.org/10.17116/molgen20244203137

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

OBJECTIVE

To study the antibacterial effect of metal nanoparticles applied by laser-induced forward transfer on a wound infection model using pig skin.

MATERIALS AND METHODS

Nanoparticles were applied by laser-induced forward transfer using a nanosecond laser. The effect of nanoparticles was studied on five bacterial strains. Scanning electron microscopy was used to visualize the treated areas of pig skin.

RESULTS AND DISCUSSIONS

Biofilm formation is considered one of the main complications of the treatment of wound infections and it is associated with the transition of wounds to a chronic non-healing state. We have presented a fundamentally new method of direct laser-induced nanoparticle transfer, which allows the infected surface to be treated with “fresh nanoparticles” obtained directly during transfer from a metal plate (silver, copper, zinc). An ex vivo model of wound infection using pig skin was obtained, which showed excellent reproducibility between repeats.

CONCLUSION

The high antimicrobial potential of this method was shown on a model of a wound infected with clinically significant bacteria: Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa.

Keywords:

ex vivo
pig skin
antibacterial nanoparticles
laser-induced direct transfer method

Authors:

Tolordava E.R.

P.N. Lebedev Physical Institute of RAS;
N.F. Gamaleya National Research Center

Nastulyavichus A.A.

P.N. Lebedev Physical Institute of RAS

Ulturgasheva E.V.

P.N. Lebedev Physical Institute of RAS

Shelygina S.N.

P.N. Lebedev Physical Institute of RAS

Saraeva I.N.

P.N. Lebedev Physical Institute of RAS

Kudryashov S.I.

P.N. Lebedev Physical Institute of RAS

Received:

26.07.2024

Accepted:

12.08.2024

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References:

  1. Wibbenmeyer L., Danks R., Faucher L., Amelon M., Latenser B., Kealey G. P., Herwaldt L. A. Prospective analysis of nosocomial infection rates, antibiotic use, and patterns of resistance in a burn population. J Burn Care Res. 2006;27:152-160.  https://doi.org/10.1097/01.BCR.0000203359.32756.F7
  2. Bowler P.G., Duerden B.I., Armstrong D.G. Wound microbiology and associated approaches to wound management. Clin. Microbiol. Rev. 2001;14:244-269.  https://doi.org/10.1128/CMR.14.2.244-269.2001
  3. Lebeaux D., Chauhan A., Rendueles O., Beloin C. From in vitro to in vivo models of bacterial biofilm-related infections. Pathogens. 2013;2:288-356.  https://doi.org/10.3390/pathogens2020288
  4. Alves D.R., Booth S.P., Scavone P., Schellenberger P., Salvage J., Dedi C., et al. Development of a High-Throughput ex-Vivo Burn Wound Model Using Porcine Skin, and Its Application to Evaluate New Approaches to Control Wound Infection. Front. Cell. Infect. Microbiol. 2018;8:15.  https://www.frontiersin.org/articles/10.3389/fcimb.2018.00196
  5. Mah T.-F.C., O Toole G.A. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol. 2001;9:34-39.  https://doi.org/10.1016/S0966-842X(00)01913-2
  6. Ryan R.P., Dow J.M. Diffusible signals and interspecies communication in bacteria. Microbiology 2008;154:1845-1858. https://doi.org/10.1099/mic.0.2008/017871-0
  7. Glushchenko N.N. Reduction of bacterial resistance to antibiotics due to their synergism with copper, Biotechnology: state and development prospects. Proceedings of the II Moscow International Congress: Abstracts of reports. — part 1. — Moscow, 2003. — p. 88 
  8. Nastulyavichus A., Tolordava E., Kudryashov S., Khmelnitskii R., Ionin A. Laser-induced transferred antibacterial nanoparticles for mixed-species bacteria biofilm inactivation. Materials. 2023;16:10.  https://doi.org/10.3390/ma16124309
  9. Nastulyavichus A., Khaertdinova L., Tolordava E., Yushina Y., Ionin A., Semenova A., Kudryashov S. Additive nanosecond laser-induced forward transfer of high antibacterial metal nanoparticle dose onto foodborne bacterial biofilms. Micromachines. 2022;13:12.  https://doi.org/10.3390/mi13122170
  10. Nastulyavichus A., Tolordava E., Rudenko A., Zazymkina D., Shakhov P., Busleev N., et al. In vitro destruction of pathogenic bacterial biofilms by bactericidal metallic nanoparticles via laser-induced forward transfer. Nanomaterials. 2020;10:11.  https://doi.org/10.3390/nano10112259
  11. Semaltianos N., Logothetidis S., Frangis N., Tsiaoussis I., Perrie W., Dearden G., Watkins K. A route to synthesize nanoparticles of titanium monoxide Laser ablation in water. Chem. Phys. Lett. 2010; 496: 113-116.  https://doi.org/10.1016/j.cplett.2010.07.023
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