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Kurko O.D.

National Research Center «Kurchatov Institute»

Davydova L.I.

National Research Center «Kurchatov Institute»”

Sidoruk K.V.

National Research Center «Kurchatov Institute»”

Grivennikov I.A.

National Research Center «Kurchatov Institute»

Debabov V.G.

National Research Center «Kurchatov Institute»”

Bogush V.G.

National Research Center «Kurchatov Institute»”;
All-Russia Research Institute of Agricultural Biotechnology

Tarantul V.Z.

National Research Center «Kurchatov Institute»

Dolotov O.V.

National Research Center «Kurchatov Institute»;
Lomonosov Moscow State University

Directed neuronal differentiation of SH-SY5Y human neuroblastoma cells on 2D matrixes containing recombinant spidroins modified with cell adhesion peptides

Authors:

Kurko O.D., Davydova L.I., Sidoruk K.V., Grivennikov I.A., Debabov V.G., Bogush V.G., Tarantul V.Z., Dolotov O.V.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2023;41(3): 16‑24

DOI: 10.17116/molgen20234103116

Read: 761 times

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Table of contents

DIRECTED NEURONAL DIFFERENTIATION OF SH-SY5Y HUMAN NEUROBLASTOMA CELLS ON 2D MATRIXES CONTAINING RECOMBINANT SPIDROINS MODIFIED WITH CELL ADHESION PEPTIDES
DIRECTED NEURONAL DIFFERENTIATION OF SH-SY5Y HUMAN NEUROBLASTOMA CELLS ON 2D MATRIXES CONTAINING RECOMBINANT SPIDROINS MODIFIED WITH CELL ADHESION PEPTIDES

To cite this article:

Kurko OD, Davydova LI, Sidoruk KV, et al. . Directed neuronal differentiation of SH-SY5Y human neuroblastoma cells on 2D matrixes containing recombinant spidroins modified with cell adhesion peptides. Molecular Genetics, Microbiology and Virology. 2023;41(3):16‑24. (In Russ.)
https://doi.org/10.17116/molgen20234103116

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

AIM

The use of efficient and inexpensive coatings (2D matrices) for the cultivation and differentiation of nerve cells in vitro is important for the development of tissue-engineered constructs intended for the treatment of pathologies of the nervous system. Promising for such constructs are recombinant analogs of the dragline silk fiber of the orb-weaving spiders web, namely, spidroins 1 and 2. The aim of the study was to evaluate the effects of coatings obtained from mixtures of recombinant spidroins (RSs) rS1/9 and rS2/12 with hybrid proteins (HPs) containing the rS1/9 monomer fused with biologically active peptides on the levels of expression of key synapse-specific genes and viability of SH-SY5Y human neuroblastoma cells in directed cholinergic differentiation.

MATERIAL AND METHODS

A two-stage scheme of directed cholinergic differentiation using retinoic acid and brain-derived neurotrophic factor (BDNF) was applied. Cell viability was assessed using the MTT test and crystal violet staining. The expression levels of the studied genes were assessed using real-time PCR.

RESULTS

Directed differentiation of SH-SY5Y cells was accompanied by a significant increase in the expression levels of the genes of synaptophysin, synapsins I, II, and the postsynaptic protein PSD-95. The highest cell viability and increased levels of PSD-95 expression were found during differentiation on a coating consisting of a mixture of RSs rS1/9 and rS2/12 with HPs containing the RGDS peptide (present in extracellular matrix proteins) and the heparin-binding peptide HBP (a fragment of laminin).

CONCLUSION

The coating consisting of a mixture of RSs rS1/9 and rS2/12 and HP based on the RS monomer rS1/9 fused with RGDS (a ligand for integrins) and HBP (a ligand for growth factors and syndecans) showed the highest efficiency in directed neuronal differentiation of SH-SY5Y cells. Coatings consisting of rS2/12 alone or a mixture of rS2/12 with HP(RGDS) demonstrated less efficiency, but the fusion with the GRGGL peptide (which interacts with NCAMs and is a fragment of rS1/9) resulted in an increase in efficiency.

Keywords:

combined matrix
recombinant spidroins
biologically active peptides
human neuroblastoma
directed neuronal differentiation
synapse-specific proteins
viability of neurons

Authors:

Kurko O.D.

National Research Center «Kurchatov Institute»

Davydova L.I.

National Research Center «Kurchatov Institute»”

Sidoruk K.V.

National Research Center «Kurchatov Institute»”

Grivennikov I.A.

National Research Center «Kurchatov Institute»

Debabov V.G.

National Research Center «Kurchatov Institute»”

Bogush V.G.

National Research Center «Kurchatov Institute»”;
All-Russia Research Institute of Agricultural Biotechnology

Tarantul V.Z.

National Research Center «Kurchatov Institute»

Dolotov O.V.

National Research Center «Kurchatov Institute»;
Lomonosov Moscow State University

Received:

06.04.2023

Accepted:

10.07.2023

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