Structure of para-magnetic dinitrosyl iron complexes with SH-ligands (DNICs) contains 2 nitrosyl groups of NO and NO⁺ which are releasing themselves under decay in the cells.

MATERIAL AND METHODS

The selective ability of diethyl dithiocarbomate (DETC) to bind NO was used to study functions of nitrosonium cation NO⁺ in biofilm regulation. A combined treatment of Pseudomonas aeruginosa with DETC and NO donors — NaNO₂ or binuclear dinitrosyl iron complex with glutathione (B-DNIC_glu) led to decreasing in biofilm rates relative to control and mono treatments with each agents.

RESULTS AND DISCUSSION

Biofilm rating depends upon the quantitative correlation between the components: under 10 fold excess of DETC against (DNIC_glu)) it decreased 3 times, while under 5 fold excess — only 1.8 times. The stable complex [Fe2⁺ — DETC] functions as the trap of NO mono oxide to block in signaling DNIC molecule syntheses through NO and intracellular iron and to accumulate NO⁺ nitrosonium cation. The most priority finding of our work was a maximum inhibition at the rate of biofilm in the options with the successive cell treatment with DETC, that followed by 40 min lag after NaNO₂ was put into the cells while DNICs — the universal signaling molecules in the biosystems- were formed intracellular.

CONCLUSION

Obtained data are contribution in study the molecular mechanisms of nitrosonium cation functions; they are correlated with our previous results in E. coli colony forming rates, as well as the data of the other authors in the yeasts and the mammalian cell culture MCF7, when the complex cell treatment with DETC and DNICs were used.