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Puzakov M.V.

A.O. Kovalevsky Institute of Biology of the Southern Seas RAS

Puzakova L.V.

A.O. Kovalevsky Institute of Biology of the Southern Seas RAS

Ulupova Y.N.

A.O. Kovalevsky Institute of Biology of the Southern Seas RAS

Differential activity of genes with fragments of IS630/TC1/MARINER transposons in the genome of MNEMIOPSIS LEIDYI

Authors:

Puzakov M.V., Puzakova L.V., Ulupova Y.N.

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Journal: Molecular Genetics, Microbiology and Virology. 2022;40(4): 30‑35

DOI: 10.17116/molgen20224004130

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To cite this article:

Puzakov MV, Puzakova LV, Ulupova YN. Differential activity of genes with fragments of IS630/TC1/MARINER transposons in the genome of MNEMIOPSIS LEIDYI. Molecular Genetics, Microbiology and Virology. 2022;40(4):30‑35. (In Russ.)
https://doi.org/10.17116/molgen20224004130

 
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GOAL

Transposable elements (TE) have a significant impact on the structure and functioning of the genome and are the source of new genes. As a result of «molecular domestication», TE genes become a functional part of the host genome. The IS630/Tc1/mariner (ITm) superfamily of DNA transposons is one of the most widespread and diverse. To date, several genes are known that appeared as a result of cooptation of ITm transposons. This work was aimed at searching for Mnemiopsis leidyi genes, in the sequence of which fragments of ITm transposons were included, as well as analyzing their expression level.

MATERIAL AND METHODS

In this work, the local alignment method (BLASTn) was used to search for hypothetical chimeric genes. The analysis of differential gene activity was assessed using the joint use of the Kallisto and Sleuth programs.

RESULTS

Not a single cDNA was found that corresponded to the complete transposase coding sequence of any ITm transposon of M. leidyi. However, 21 unique transcripts of hypothetical chimeric genes with regions homologous to ITm transposons were found. Transcriptional analysis showed that during early embryonic development, as well as in the tissues of the ridges and epithelium of adult animals, the predominant proportion of hypothetical chimeric genes is not expressed, or is expressed at a low level. However, at several loci, a differential level of activity is recorded during the regeneration process.

CONCLUSION

Differential activity of individual hypothetical chimeric genes during regeneration may indicate their possible “domestication” and involvement in the molecular genetic processes of ctenophores.

Keywords:

molecular domestication
DNA transposons
chimeric genes
transcriptional activity

Authors:

Puzakov M.V.

A.O. Kovalevsky Institute of Biology of the Southern Seas RAS

Puzakova L.V.

A.O. Kovalevsky Institute of Biology of the Southern Seas RAS

Ulupova Y.N.

A.O. Kovalevsky Institute of Biology of the Southern Seas RAS

Received:

03.12.2021

Accepted:

15.04.2022

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