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Zakharova I.B.

Department of Microbiology, Volgograd Plague Control Research Institute

Vasilyeva K.V.

Department of Microbiology, Volgograd Plague Control Research Institute

Bui L.A-T.

Department of Tropical Medicine Russian-Vietnamese Tropical Research Center

Victorov A.D.

Department of Microbiology, Volgograd Plague Control Research Institute

Shpak I.M.

Department of Microbiology, Volgograd Plague Control Research Institute

Viktorov D.V.

Department of Microbiology, Volgograd Plague Control Research Institute

Genetic and morphological diversity of vietnamese population of Burkholderia thailandensis

Authors:

Zakharova I.B., Vasilyeva K.V., Bui L.A-T., Victorov A.D., Shpak I.M., Viktorov D.V.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2022;40(1): 37‑45

DOI: 10.17116/molgen20224001137

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Table of contents

GENETIC AND MORPHOLOGICAL DIVERSITY OF VIETNAMESE POPULATION OF BURKHOLDERIA THAILANDENSIS
GENETIC AND MORPHOLOGICAL DIVERSITY OF VIETNAMESE POPULATION OF BURKHOLDERIA THAILANDENSIS

To cite this article:

Zakharova IB, Vasilyeva KV, Bui LA-T, Victorov AD, Shpak IM, Viktorov DV. Genetic and morphological diversity of vietnamese population of Burkholderia thailandensis. Molecular Genetics, Microbiology and Virology. 2022;40(1):37‑45. (In Russ.)
https://doi.org/10.17116/molgen20224001137

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

INTRODUCTION

Burkholderia thailandensis and B. pseudomallei are closely related gram-negative saprophytic microbes. Recently, the variant strains of B. thailandensis bearing a capsular polysaccharide gene cluster (Bp-like CPS) exhibiting high similarity to the corresponding cluster in B. pseudomallei were discovered. In the present study, we aimed to determine the presence of B. thailandensis capsular variant strains (BTCV) in the environment of Vietnam.

METHODS

Multilocus species-specific PCR and MLST used to identify the strains. 87 B. thailandensis soil isolates from Southern and Central Vietnam were investigated.

RESULTS

The six pairs of primers were designed to detect genes of Bp-like CPS cluster. 11 BTCV strains have been revealed. The strains bearing Bp-like CPS cluster formed mainly a pseudomallei-like rough and wrinkled colonies.

CONCLUSION

Also found that L1629S and T1942A polymorphisms in type I polyketide synthase WcbR are closely associated with pseudomallei-like colony morphology in EPS-expressing strains of the main population of B. thailandensis in Vietnam. The obtained data indicate a high degree of genetic heterogeneity accompanied by phenotypic diversity of B. thailandensis in Vietnam.

Keywords:

Burkholderia thailandensis
B.pseudomallei
Vietnam
BTCV
Bp-like CPS
type I polyketide synthase
Burkholderia thailandensis
B.pseudomallei
Vietnam
BTCV
Bp-like CPS
type I polyketide synthase

Authors:

Zakharova I.B.

Department of Microbiology, Volgograd Plague Control Research Institute

Vasilyeva K.V.

Department of Microbiology, Volgograd Plague Control Research Institute

Bui L.A-T.

Department of Tropical Medicine Russian-Vietnamese Tropical Research Center

Victorov A.D.

Department of Microbiology, Volgograd Plague Control Research Institute

Shpak I.M.

Department of Microbiology, Volgograd Plague Control Research Institute

Viktorov D.V.

Department of Microbiology, Volgograd Plague Control Research Institute

Received:

05.06.2020

Accepted:

15.11.2020

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