Multilocus sequence analysis of isolates from patients with erythemic form of ixodid tick-borne borrelioses

Golidonova K.A.; Korenberg E.I.; Gorelova N.B.; Ginzburg A.L.

doi:https://doi.org/10.17116/molgen20213904114

Golidonova K.A.

National Research Center for Epidemiology and Microbiology named after the honorary academician N. F. Gamaleya

Korenberg E.I.

National Research Center for Epidemiology and Microbiology named after the honorary academician N. F. Gamaleya

Gorelova N.B.

National Research Center for Epidemiology and Microbiology named after the honorary academician N. F. Gamaleya

Ginzburg A.L.

National Research Center for Epidemiology and Microbiology named after the honorary academician N. F. Gamaleya

Multilocus sequence analysis of isolates from patients with erythemic form of ixodid tick-borne borrelioses

Authors:

Golidonova K.A., Korenberg E.I., Gorelova N.B., Ginzburg A.L.

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Journal: Molecular Genetics, Microbiology and Virology. 2021;39(4): 14‑20

DOI: 10.17116/molgen20213904114

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To cite this article:

Golidonova KA, Korenberg EI, Gorelova NB, Ginzburg AL. Multilocus sequence analysis of isolates from patients with erythemic form of ixodid tick-borne borrelioses. Molecular Genetics, Microbiology and Virology. 2021;39(4):14‑20. (In Russ.)
https://doi.org/10.17116/molgen20213904114

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)

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Abstract:

Ixodid tick-borne borrelioses (ITBB) is widespread in Russia and is ranked first among all natural focal infections in terms of morbidity. More than 50% of them are erythemic.

THE AIM OF THE STUDY

Is to clarify the taxonomic status of one of the causative agents of the erythemic form of ITBB in Eastern Europe.

MATERIALS AND METHODS

The method of multilocus sequence analysis (MLSA) was used to study 23 primary isolates of Borrelia obtained by inoculating skin biopsies on BSK medium, as well as blood plasma from patients with erythemic form of ITBB infected in the Perm region of Russia.

RESULTS

According to the MLSA results, the nucleotide sequences of the studied Borrelia isolates differed by at least 2.2—2.7% from the sequences of B. garinii 20047T and turned out to be similar to B. bavariensis PBi by at least 98.3%. The sequences of these isolates were almost identical (98.5—100%) to the strains NT29, SZ, Prm7564-11, Prm7569-11, and Hiratsuka of the eurasian genomic subgroup of B. bavariensis, which made it possible to assign them to the same subgroup. Among the investigated Borrelia isolates, two genovariants were identified that differ from each other by more than 1% in nucleotide sequences, while the similarity between the sequences of each genovariant was 99.7—100%.

CONCLUSION

Along with B. garinii and B. afzelii, B. bavariensis is widespread in Eurasia and is of great importance as the causative agent of ITBB. Eurasian and european genomic subgroups of this pathogen circulate in Russia. The eurasian genomic subgroup of B. bavariensis can cause the erythemic form of the ITBB.

Keywords:

Ixodid tick-borne borrelioses

etiology

Borrelia bavariensis

isolates

skin biopsies

blood plasma

MLSA

Authors:

Golidonova K.A.

National Research Center for Epidemiology and Microbiology named after the honorary academician N. F. Gamaleya

Korenberg E.I.

National Research Center for Epidemiology and Microbiology named after the honorary academician N. F. Gamaleya

Gorelova N.B.

National Research Center for Epidemiology and Microbiology named after the honorary academician N. F. Gamaleya

Ginzburg A.L.

National Research Center for Epidemiology and Microbiology named after the honorary academician N. F. Gamaleya

Received:

25.12.2020

Accepted:

18.02.2021

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References:

Margos G, Castillo-Ramirez S, Cutler S, Dessau RB, Eikeland R, Estrada-Peña A et al. Rejection of the name Borreliella and all proposed species comb. nov. placed therein. Int J Syst Evol Microbiol. 2020;70(5):3577-3581. https://doi.org/10.1099/ijsem.0.004149
Nefedova V, Korenberg E & Gorelova N. Multilocus Sequence Analysis of «Atypical» Borrelia burgdorferi sensu lato Isolated in Russia. Molecular Genetics, Microbiology and Virology. 2017;32:196-203. https://doi.org/10.3103/S0891416817040073
Kurkatova LV, Korenberg EI, Gorelova NB. Modern condition of the dominic museum of borrely. Zdorov’e naselenija i sreda obitanija. 2017;2(290):54-56. (In Russ.). https://doi.org/10.35627/2219-5238/2017-290-5-54-56
Adeolu M, Gupta RS. A phylogenomic and molecular marker based proposal for the division of the genus Borrelia into two genera: the emended genus Borrelia containing only the members of the relapsing fever Borrelia, and the genus Borreliella gen. nov. containing the members of the Lyme disease Borrelia (Borrelia burgdorferi sensu lato complex). Antonie Van Leeuwenhoek. 2014;105(6):1049-1072. https://doi.org/10.1007/s10482-014-0164-x
Margos G, Vollmer SA, Cornet M, Garnier M, Fingerle V, Wilske B et al. A new Borrelia species defined by multilocus sequence analysis of housekeeping genes. Appl. Environmental Microbiology. 2009;75(16):5410-5416. https://doi.org/10.1128/AEM.00116-09
Margos G, Wilske B, Sing A, Hizo-Teufel A, Cao WC, Chu C et al. Borrelia bavariensis sp. nov. is widely distributed in Europe and Asia. International Journal of Systematic and Evolutionary Microbiology. 2013;64:4284-4288. https://doi.org/10.1099/ijs.0.052001-0
Richter D, Postic D, Sertour N, Livey I, Matuschka FR, Baranton G. Delineation of Borrelia burgdorferi sensu lato species by multilocus sequence analysis and confirmation of the delineation of Borrelia spielmanii sp. nov. International Journal of Systematic and Evolutionary Microbiology. 2006;56(Pt4):873-881. https://doi.org/10.1099/ijs.0.64050-0
Takano A, Nakao M, Masuzawa T, Takada N, Yano Y, Ishiguro F et al. Multilocus Sequence Typing Implicates Rodents as the Main Reservoir Host of Human-Pathogenic Borrelia garinii in Japan. Journal of Clinical Microbiology. 2011;49(5):2035-2039. https://doi.org/10.1128/JCM.02544-10
Scholz HC, Margos G, Derschum H, Speck S, Tserennorov D, Erdenebat N et al. High prevalence of genetically diverse Borrelia bavariensis-like strains in Ixodes persulcatus from Selenge Aimag, Mongolia. Ticks and tick-borne diseases. 2013;4(1-2):89-92. https://doi.org/10.1016/j.ttbdis.2012.08.004
Margos G, Fingerle V and Reynolds S. Borrelia bavariensis: Vector Switch, Niche Invasion, and Geographical Spread of a Tick-Borne Bacterial Parasite. Frontiers in Ecology and Evolution. 2019;7:401. https://doi.org/10.3389/fevo.2019.00401
Korenberg EI, Nefedova VV, Gorelova NB, Kovalevsky YV, Fadeeva IA, Golubova DA. The etiological structure of combined southern taiga natural foci of ixodic tick-borne borreliosis. Vestnik RAMN. 2011;10:10-14. (In Russ.).
Rukovodstvo po meditsinskoi mikrobiologii. Chastnaya meditsinskaya mikrobiologiya i ehtiologicheskaya diagnostika infektsii. Pod red. Labinskaya A.S., Kostyukova N.N., Ivanova S.M.: M. Binom; 2010;844-866. (In Russ.).
Korenberg EI, Pomelova VG, Osin NS. Infections with Natural Focality Transmitted by IXODID TICKS. M.: Komentarij; 2013. (In Russ.).
Korenberg EI, Vorobyeva NN, Sumlivaya ON, Frizen VI, Afanasyeva MB. Infections transmitted by ixodid ticks in the Perm Territory (etiology, epidemiology, pathogenesis, clinic, diagnosis, treatment and prevention). Methodical recommendations for doctors. Perm’: [b. v.]; 2007. (In Russ.).
Nikolenko VV, Vorobyova NN, Merkuryeva EV, Nikolenko AV, Suslina OA. Lyme borreliosis: clinical and epidemiological features of its course in the territory of large industrial center of Western Urals. Perm Medical J. 2020;4(37):46-53. (In Russ.). https://doi.org/10.17816/pmj37446-53
Vorobyeva NN, Sumlivaya ON. Klinicheskie varianty iksodovykh kleshchevykh borreliozov v ostrom periode zabolevaniya. Medical parasitology and parasitic diseases. 2003;4(203):3-7. (In Russ.).
Nefedova V, Korenberg E, Gorelova N, Genetic variants of Borrelia garinii, a widespread Eurasian pathogen of ixodid tick borreliosis. Molecular genetics, microbiology, and virology. 2010;25(3):95-100. https://doi.org/10.3103/S089141681003002X
Nefedova VV, Teterin VY, Korenberg EI, Gorelova NB, Vorobyeva NN, Frizen VI. Isolation of the causative agent of ixodic tick-borne borreliosis from the blood of patients. Zhurnal mikrobiologii, èpidemiologii i immunobiologii. 2009;1:63-66. (In Russ.).
Vorobyeva NN, Korenberg EI, Gorelova NB, Postic D, Zapyantsev AN. Preliminary data on the etiology of the erythemal form of ixodic tick-borne borreliosis in the Perm region. In: All-Russian scientific-practical conference of Omsk Research Institute of Natural Focal Infections of the Ministry of Health of Russia «Natural focal infections in Russia: modern epidemiology, diagnostics, tactics of protecting the population». Omsk; 1998 Oct 20-21; p. 77-78. Russia: Omsk Research Institute of Natural Focal Infections of the Ministry of Health of Russia; 1998. (In Russ.).
Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. 1987;4:406-425.
Becker NS, Rollins RE, Nosenko K, et al. High conservation combined with high plasticity: genomics and evolution of Borrelia bavariensis. BMC Genomics. 2020;21:702. https://doi.org/10.1186/s12864-020-07054-3
Mukhacheva T, Kovalev S. Multilocus sequence analysis of Borrelia burgdorferi s.l. in Russia. Ticks and tick-borne diseases. 2013;4(4):275-279. https://doi.org/10.1016/j.ttbdis.2013.02.004
Korenberg E, Gorelova N, Kovalevskii Y. Ecology of Borrelia burgdorferi sensu lato in Russia. In: Gray J, Kahl O, Lane R and Stanek G, editors. Lyme borreliosis: biology, epidemiology and control. CAB International. Oxford. 2002;175-200.
Korenberg E, Nefedova V, Fadeeva I, Gorelova N. Genotyping of Borrelia burgdorferi sensu lato in Russia. In: Cabello F, Hulinska D and Godfrey H editors. «Molecular Biology of Spirochetes». IOS Press. Amsterdam, Berlin, Oxford, Tokyo, Washington; 2006;174-199.
Mukhacheva T, Kovalev S. Borrelia spirochetes in Russia: Genospecies differentiation by real-time PCR. Ticks and Tick-borne Diseases. 2014;5(6):722-726. https://doi.org/10.1016/j.ttbdis.2014.05.016
Sabitova Y, Fomenko N, Tikunov A, Stronin O, Khasnatinov M, Abmed D et al. Multilocus sequence analysis of Borrelia burgdorferi sensu lato isolates from Western Siberia, Russia and Northern Mongolia. Infection, Genetics and Evolution. 2018;62:160-169. https://doi.org/10.1016/j.meegid.2018.04.015
Rar V, Livanova N, Tkachev S, Galina K, Tikunov A, Sabitova Y, et al. Detection and genetic characterization of a wide range of infectious agents in Ixodes pavlovskyi ticks in Western Siberia, Russia. Parasites Vectors. 2017;10:258. https://doi.org/10.1186/s13071-017-2186-5
Nefedova VV, Korenberg EI, Gorelova NB, Kovalevsky YV. Geneticheskaya geterogennost’ Borrelia garinii v prirodnom ochage Srednego Urala. Byulleten’ VSNTS SO RAMN. 2007;3(55):139-142. (In Russ.).
Postic D, Korenberg E, Gorelova N, Kovalevski Y, Bellenger E, Baranton G. Borrelia burgdorferi sensu lato in Russia and neighbouring countries: high incidence of mixed isolates [published correction appears in Res Microbiol. 1998;149(1):73]. Research in Microbiology. 1997;148(8):691-702. https://doi.org/10.1016/S0923-2508(99)80068-0
Hao Q, Hou X, Geng Z & Wan K. Distribution of Borrelia burgdorferi sensu lato in China. Journal of clinical microbiology. 2011;49(2):647-650. https://doi.org/10.1128/JCM.00725-10