Molecular genetic characteristics of polyresistant Listeria monocytogenes strains and identification of new sequence types

Astashkin E.I.; Alekseeva E.A.; Borzenkov V.N.; Kislichkina A.A.; Mukhina T.N.; Platonov M.E.; Svetoch E.A.; Shepelin A.P.; Fursova N.K.

doi:https://doi.org/10.17116/molgen2021390413

Astashkin E.I.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Alekseeva E.A.

The Center for Hygiene and Epidemiology in the Vologda Region Rospotrebnadzor

Borzenkov V.N.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Kislichkina A.A.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Mukhina T.N.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Platonov M.E.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Svetoch E.A.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Shepelin A.P.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Fursova N.K.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Molecular genetic characteristics of polyresistant Listeria monocytogenes strains and identification of new sequence types

Authors:

Astashkin E.I., Alekseeva E.A., Borzenkov V.N., Kislichkina A.A., Mukhina T.N., Platonov M.E., Svetoch E.A., Shepelin A.P., Fursova N.K.

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Journal: Molecular Genetics, Microbiology and Virology. 2021;39(4): 3‑13

DOI: 10.17116/molgen2021390413

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To cite this article:

Astashkin EI, Alekseeva EA, Borzenkov VN, et al. . Molecular genetic characteristics of polyresistant Listeria monocytogenes strains and identification of new sequence types. Molecular Genetics, Microbiology and Virology. 2021;39(4):3‑13. (In Russ.)
https://doi.org/10.17116/molgen2021390413

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)

Использование инфографики авторами научных работ

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Abstract:

INTRODUCTION

Listeria monocytogenes is a causative agent of listeriosis with a lethality rate of up to 21%.

THE GOAL

Is to study the genotypes and phenotypes of 30 L. monocytogenes strains isolated in 8 regions of the Russian Federation in 2015—2018 from people, from food and from waste water.

MATERIALS AND METHODS

Susceptibility to antimicrobials for L. monocytogenes strains, serotyping, MLST-typing, whole-genome sequencing, identification of antibiotic resistance genes, virulence genes and stress survival islets genes, and phylogenetic analysis were done.

RESULTS

All strains are classified as multidrug-resistant (MDR), resistance genes fosX, pbp-like, lin, norB and sul were identified, their allelic profiles were determined. Sixteen L. monocytogenes sequence types (ST) were identified: four STs belonging to evolutionary line I and twelve STs to evolutionary line II, including 13 previously known STs (ST1, ST2, ST5, ST7, ST9, ST14, ST16, ST20, ST26, ST121, ST155, ST425 and ST451) and three novel STs (ST1453, ST1455 and ST1454). Five serogroups were revealed: 1-2a-3a, 1/2c-3c, 4b, 4a-4c and 1/2b-3b-7. LIPI-1 pathogenicity island (prfA, hly, plcA, plcB, mpl, actA) and LIPI-2 pathogenicity island (inlABCJ) were identified in the genomes of all strains. LIPI-3 pathogenicity island (llsAXGHBYDP) was identified in two strains of the evolutionary line I. SSI-1 and SSI-2 stress survival islets were found in nine and one strains, respectively. Allelic profiles of virulence and stress resistance genes were determined. Phylogenetic analysis revealed a large genetic diversity of the strains.

CONCLUSION

L. monocytogenes MDR strains belonging to widespread and highly virulent sequence types, were identified in the study, three new sequence types were described.

Keywords:

Listeria monocytogenes

MDR

sequence types

pathogenicity islands

LIPI-1

LIPI-2

LIPI-3

stress survival islets

SSI-1

SSI-2

Authors:

Astashkin E.I.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Alekseeva E.A.

The Center for Hygiene and Epidemiology in the Vologda Region Rospotrebnadzor

Borzenkov V.N.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Kislichkina A.A.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Mukhina T.N.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Platonov M.E.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Svetoch E.A.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Shepelin A.P.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Fursova N.K.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Received:

16.07.2020

Accepted:

01.09.2020

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