Change in the clonal structure of carbapenem not susceptible Acinetobacter baumannii isolated from the blood culture of patients with hematological malignancies

Khrulnova S.A.; Korobova A.G.; Fedorova A.V.; Frolova I.N.; Klyasova G.A.

doi:https://doi.org/10.17116/molgen202038031120

Khrulnova S.A.

National Research Center for Hematology

Korobova A.G.

National Research Center for Hematology

Fedorova A.V.

National Research Center for Hematology

Frolova I.N.

National Research Center for Hematology

Klyasova G.A.

National Research Center for Hematology

Change in the clonal structure of carbapenem not susceptible Acinetobacter baumannii isolated from the blood culture of patients with hematological malignancies

Authors:

Khrulnova S.A., Korobova A.G., Fedorova A.V., Frolova I.N., Klyasova G.A.

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Journal: Molecular Genetics, Microbiology and Virology. 2020;38(3): 120‑127

DOI: 10.17116/molgen202038031120

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Khrulnova SA, Korobova AG, Fedorova AV, Frolova IN, Klyasova GA. Change in the clonal structure of carbapenem not susceptible Acinetobacter baumannii isolated from the blood culture of patients with hematological malignancies. Molecular Genetics, Microbiology and Virology. 2020;38(3):120‑127. (In Russ.)
https://doi.org/10.17116/molgen202038031120

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)

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Abstract:

INTRODUCTION

The emergence and dissemination of sucsessful clones of A. baumannii is a serious problem for the medical community.

OBJECTIVE

To investigate clonal diversity of A.baumannii isolated from blood culture in patients with hematological malignancies.

MATERIAL AND METHODS

The study included A.baumannii isolated from blood culture in patients in 7 Russian hospitals (2003—2017). Two multiplex PCRs were performed to selectively amplify group 1 or group 2 alleles of the ompA, csuE, and bla_OXA-51-like. Isolates were assigned to one of the sequence groups (G1—G14) according to allelic profiles.

RESULTS

A total of 96 A.baumannii were examined of those 77 (80.2%) were non-susceptible to meropenem and/or imipenem. Genes of acquired carbapenemases were detected in 79.2% (61/77) carbapenem non-susceptible isolates. The prevalent carbapenemase genes were bla_{OXA-24/40-like} (45.9%) and bla_OXA-23-like (45.9%), followed by bla_OXA-58-like (6.6%), co-harboring bla_{OXA-24/40-like} and bla_OXA-23-like (1.6%). During the study period the rate of bla_OXA-23-like-carrying A.baumannii increased from 30% (2003—2010) up to 53.7% (2011—2017), whereas A.baumannii harboring bla_{OXA-24/40-like} decreased from 50% (2003—2010) to 43.9% (2011—2017). The majority of A.baumannii carrying acquired carbapenemase genes (62.3%) belonged to G1 group (international clone II, IC II) which is widespread in the world. G1 (IC II) isolates harboring bla_OXA-23-like were not detected during 2003—2010 whereas in 2011—2017 G1 (IC II) A.baumannii carrying bla_OXA-23-like accounted for 86.4%. During the study period, there was a slight decrease in the rate of bla_{OXA-24/40-like}-carrying A.baumannii from 70% (2003—2010) to 53.7% (2011—2017).

CONCLUSIONS

The increase of G1 (ICII) A.baumannii harboring bla_OXA-23-like was observed during last years (2011—2017).

Keywords:

Acinetobacter baumannii

blood culture

OXA-carbapenemases

patients with hematological malignancies

international clonal lineages

Authors:

Khrulnova S.A.

National Research Center for Hematology

Korobova A.G.

National Research Center for Hematology

Fedorova A.V.

National Research Center for Hematology

Frolova I.N.

National Research Center for Hematology

Klyasova G.A.

National Research Center for Hematology

Received:

21.06.2019

Accepted:

20.07.2019

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References:

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