Peculiarities of the Deinococcus radiodurans r1 uvrD coded DNA helicase revealed in Escherichia coli k-12 cells

Gulevich E.P.; Kuznetsova L.V.; Kil Yu.V.; Verbenko V.N.

doi:https://doi.org/10.17116/molgen20203801134

Gulevich E.P.

Petersburg Nuclear Physics Institute by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Gatchina, Leningrad Region, Russia

Kuznetsova L.V.

Federal'nyĭ tsentr serdtsa, krovi i éndokrinologii im. V.A. Almazova Minzdravsotsrazvitiia Rossii, Institut perinatologii i pediatrii, NKO reproduktsii i zdorov'ia zhenshchiny, Sankt-Peterburg

Kil Yu.V.

Petersburg Nuclear Physics Institute by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Gatchina, Leningrad Region, Russia

Verbenko V.N.

Petersburg Nuclear Physics Institute by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Gatchina, Leningrad Region, Russia

Peculiarities of the Deinococcus radiodurans r1 uvrD coded DNA helicase revealed in Escherichia coli k-12 cells

Authors:

Gulevich E.P., Kuznetsova L.V., Kil Yu.V., Verbenko V.N.

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Journal: Molecular Genetics, Microbiology and Virology. 2020;38(1): 34‑39

DOI: 10.17116/molgen20203801134

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Gulevich EP, Kuznetsova LV, Kil YuV, Verbenko VN. Peculiarities of the Deinococcus radiodurans r1 uvrD coded DNA helicase revealed in Escherichia coli k-12 cells. Molecular Genetics, Microbiology and Virology. 2020;38(1):34‑39. (In Russ.)
https://doi.org/10.17116/molgen20203801134

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)

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Abstract:

Introduction. Reparative helicase II of Deinococcus radiodurans performs an unexpected critical function in repair of double strand breaks of DNA by the mechanism of extended synthesis-dependent strand annealing while it is considered as an optional participant in the RecF pathway of recombinational repair in Escherichia coli. Materials and methods. Fragment of genome DNA of radioresistant bacterium Deinococcus radiodurans harboring the uvrD gene coding for DNA helicase II was cloned in cells of a model organism — Escherichia coli. Results. Plasmid pCR 2.1-uvrD+ restores radiation resistance of Escherichia coli uvrD, helD and rep mutants defective in reparative helicase II, helicase IV and replicative helicase Rep, correspondingly, practically up to wild type AB1157 (uvrD+) level and in less extent strains with recQ mutation in the key helicase of recombinational repair RecQ. The protective effect is also noticeable if strains with plasmid are irradiated by g-rays. Conclusion. Wide functional possibility of D. radiodurans UvrD helicase is established.

Keywords:

DNA helicase II

Deinococcus radiodurans

uvrD

rep

recQ

helD

functional complementation

radiation resistance

Authors:

Gulevich E.P.

Petersburg Nuclear Physics Institute by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Gatchina, Leningrad Region, Russia

Kuznetsova L.V.

Federal'nyĭ tsentr serdtsa, krovi i éndokrinologii im. V.A. Almazova Minzdravsotsrazvitiia Rossii, Institut perinatologii i pediatrii, NKO reproduktsii i zdorov'ia zhenshchiny, Sankt-Peterburg

Kil Yu.V.

Petersburg Nuclear Physics Institute by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Gatchina, Leningrad Region, Russia

Verbenko V.N.

Petersburg Nuclear Physics Institute by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Gatchina, Leningrad Region, Russia

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