The process of biofilm formation by Mycoplasma pneumoniae Fh on the abiotic surface by light and electron microscopy in dynamics was studied. Quantitative assessment of the effectiveness of biofilm formation were performed on polystyrene 96 well Cell Culture Clusters and staining with the crystal-Violet. For the analysis of the ultrastructural organization of Mp cells, the scanning electron microscopy method was used. To the third day of cultivation, it revealed a well-formed multilayer structure, significant in size, clearly delineated, in which Mp cells were combined with an extracellular matrix and a system of well-detected pores. The morphology of the detected formations was completely identical to the structure of biofilms described for other microorganisms, which confirms the ability of Mp to form It. The described phenomenon — the ability of Mp to grow as a biofilm — is of great importance both in understanding the causes of long-term persistence of Mp in the human body, and in developing new approaches to the treatment of protracted and chronic forms of infectious processes caused by Mp.