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Lippi G.

Section of Clinical Biochemistry, University of Verona, Verona, Italy

Betsou F.

Integrated Biobank of Luxembourg, Luxembourg, Luxembourg

Cadamuro J.

Department of Laboratory Medicine, University Hospital Salzburg, Paracelsus Medical University, Salzburg, Austria

Cornes M.

Worcestershire Acute Hospitals NHS Trust, Worcester, UK

Fleischhacker M.

DRK Kliniken Berlin Mitte, Berlin, Germany

Fruekilde P.

Odense University Hospital, Odense, Denmark

Neumaier M.

Institute for Clinical Chemistry of Heidelberg University, Mannheim, Germany

Nybo M.

Odense University Hospital, Odense, Denmark

Padoan A.

Department of Laboratory Medicine, University-Hospital of Padova, Via Giustiniani 2, 35128 Padova, Italy

Plebani M.

Department of Laboratory medicine, University-Hospital of Padova-Italy

Sciacovelli L.

University Hospital of Padova, Padova, Italy

Vermeersch P.

University Hospitals Leuven, Leuven, Belgium

von Meyer A.

Kliniken Nordoberpfalz, Weiden, Germany;
Klinikum St. Marien, Amberg, Germany

Simunic A-M.

Clinical Hospital "Sveti Duh", Zagreb, Croatia

Preanalytical challenges — time for solutions

Authors:

Lippi G., Betsou F., Cadamuro J., Cornes M., Fleischhacker M., Fruekilde P., Neumaier M., Nybo M., Padoan A., Plebani M., Sciacovelli L., Vermeersch P., von Meyer A., Simunic A-M.

More about the authors

Journal: Laboratory Service. 2020;9(2): 36‑44

DOI: 10.17116/labs2020902136

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To cite this article:

Lippi G, Betsou F, Cadamuro J, et al. . Preanalytical challenges — time for solutions. Laboratory Service. 2020;9(2):36‑44.
https://doi.org/10.17116/labs2020902136

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The European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for the Preanalytical Phase (WG-PRE) was originally established in 2013, with the main aims of (i) promoting the importance of quality in the preanalytical phase of the testing process, (ii) establishing best practices and providing guidance for critical activities in the preanalytical phase, (iii) developing and disseminating European surveys for exploring practices concerning preanalytical issues, (iv) organizing meetings, workshops, webinars or specific training courses on preanalytical issues. As education is a core activity of the WG-PRE, a series of European conferences have been organized every second year across Europe. This collective article summarizes the leading concepts expressed during the lectures of the fifth EFLM Preanalytical Conference “Preanalytical Challenges – Time for solutions”, held in Zagreb, 22–23 March, 2019. The topics covered include sample stability, preanalytical challenges in hematology testing, feces analysis, bio-banking, liquid profiling, mass spectrometry, next generation sequencing, laboratory automation, the importance of knowing and measuring the exact sampling time, technology aids in managing inappropriate utilization of laboratory resources, management of hemolyzed samples and preanalytical quality indicators.

Keywords:

education
errors
laboratory medicine
preanalytical phase
quality

Authors:

Lippi G.

Section of Clinical Biochemistry, University of Verona, Verona, Italy

Betsou F.

Integrated Biobank of Luxembourg, Luxembourg, Luxembourg

Cadamuro J.

Department of Laboratory Medicine, University Hospital Salzburg, Paracelsus Medical University, Salzburg, Austria

Cornes M.

Worcestershire Acute Hospitals NHS Trust, Worcester, UK

Fleischhacker M.

DRK Kliniken Berlin Mitte, Berlin, Germany

Fruekilde P.

Odense University Hospital, Odense, Denmark

Neumaier M.

Institute for Clinical Chemistry of Heidelberg University, Mannheim, Germany

Nybo M.

Odense University Hospital, Odense, Denmark

Padoan A.

Department of Laboratory Medicine, University-Hospital of Padova, Via Giustiniani 2, 35128 Padova, Italy

Plebani M.

Department of Laboratory medicine, University-Hospital of Padova-Italy

Sciacovelli L.

University Hospital of Padova, Padova, Italy

Vermeersch P.

University Hospitals Leuven, Leuven, Belgium

von Meyer A.

Kliniken Nordoberpfalz, Weiden, Germany;
Klinikum St. Marien, Amberg, Germany

Simunic A-M.

Clinical Hospital "Sveti Duh", Zagreb, Croatia

List of references:

  1. Cornes MP, Church S, van Dongen-Lases E, Grankvist K, Guimarães JT, Ibarz M, et al. The role of European Federation of Clinical Chemistry and Laboratory Medicine Working Group for Preanalytical Phase in standardization and harmonization of the preanalytical phase in Europe. Ann Clin Biochem. 2016;53:539-547. https://doi.org/10.1177/0004563216643969
  2. Lippi G, Simundic AM. The EFLM strategy for harmonization of the preanalytical phase. Clin Chem Lab Med. 2018;56:1660-1666. https://doi.org/10.1515/cclm-2017-0277
  3. Lippi G, Chance JJ, Church S, Dazzi P, Fontana R, Giavarina D, et al. Preanalytical quality improvement: from dream to reality. Clin Chem Lab Med. 2011;49:1113-1126. https://doi.org/10.1515/CCLM.2011.600
  4. Lippi G, Becan-McBride K, Behúlová D, Bowen RA, Church S, Delanghe J, et al. Preanalytical quality improvement: in quality we trust. Clin Chem Lab Med. 2013;51:229-241. https://doi.org/10.1515/cclm-2012-0597
  5. Lippi G, Banfi G, Church S, Cornes M, De Carli G, Grankvist K, et al. Preanalytical quality improvement. In pursuit of harmony, on behalf of European Federation for Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for Preanalytical Phase (WG-PRE). Clin Chem Lab Med. 2015;53:357-370. https://doi.org/10.1515/cclm-2014-1051
  6. Lippi G, Baird GS, Banfi G, Bölenius K, Cadamuro J, Church S, et al. Improving quality in the preanalytical phase through innovation, on behalf of the European Federation for Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for Preanalytical Phase (WG-PRE). Clin Chem Lab Med. 2017;55:489-500. Web of Science Google Scholar.
  7. Hawker CD. Nonanalytic laboratory automation: a quarter century of progress. Clin Chem. 2017;63:1074-1082. Web of Science Crossref Pub Med Google Scholar.
  8. Lou AH, Elnenaei MO, Sadek I, Thompson S, Crocker BD, Nassar B. Evaluation of the impact of a total automation system in a large core laboratory on turnaround time. Clin Biochem. 2016;49:1254. Crossref Web of Science Google Scholar.
  9. Carraro P, Plebani M. Errors in a stat laboratory: types and frequencies 10 years later. Clin Chem. 2007;53:1338-1342. Web of Science Crossref Google Scholar.
  10. Jensen E, Blaabjerg O, Petersen PH, Hegedüs L. Sampling time is important but may be overlooked in establishment and use of thyroid-stimulating hormone reference intervals. Clin Chem. 2007;53:355-356. Web of Science Pub Med Google Scholar.
  11. Schumacher GE. Choosing optimal sampling times for therapeutic drug monitoring. Clin Pharm. 1985;4:84-92. Pub Med Google Scholar.
  12. Montagnana M, Salvagno GL, Lippi G. Circadian variation within hemostasis: an underrecognized link between biology and disease? Semin Thromb Hemost. 2009;35:23-33. Pub Med Crossref Web of Science Google Scholar.
  13. Cadamuro J, Gaksch M, Wiedemann H, Lippi G, von Meyer A, Pertersmann A, et al. Are laboratory tests always needed? Frequency and causes of laboratory overuse in a hospital setting. Clin Biochem. 2018;54:85-91. Crossref Web of Science Google Scholar.
  14. Horvath A. From evidence to best practice in laboratory medicine. Clin Biochem Rev. 2013;34:47-60. Pub Med Google Scholar.
  15. Cadamuro J, Ibarz M, Cornes M, Nybo M, Haschke-Becher E, von Meyer A, et al. Managing inappropriate utilization of laboratory resources. Diagnosis (Berl). 2019;6:5-13. Pub Med Google Scholar
  16. Buoro S, Lippi G. Harmonization of laboratory hematology: a long and winding journey. Clin Chem Lab Med. 2018;56:1575-1578. Pub Med Web of Science Crossref Google Scholar.
  17. Banfi G, Salvagno GL, Lippi G. The role of ethylenediamine tetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes. Clin Chem Lab Med. 2007;45:565-576. Pub Med Google Scholar.
  18. Lippi G, Plebani M. EDTA-dependent pseudothrombocytopenia: further insights and recommendations for prevention of a clinically threatening artifact. Clin Chem Lab Med. 2012;50:1281-1285. Pub Med Web of ScienceGoogle Scholar.
  19. Rapi S, Cellai F, Rubeca R. Is it possible to correctly assess the pre-analytical characteristics of faecal tests? J Lab Precis Med. 2018;3:1. Google Scholar.
  20. Padoan A, D’ Incà R, Scapellato ML, De Bastiani R, Caccaro R, Mescoli C, et al. Improving IBD diagnosis and monitoring by understanding preanalytical, analytical and biological fecal calprotectin variability. Clin Chem Lab Med. 2018;56:1926-1935. Crossref Pub Med Web of Science Google Scholar.
  21. Cadamuro J, Simundic AM, Ajzner E, Sandberg S. A pragmatic approach to sample acceptance and rejection. Clin Biochem. 2017;50:579-581. Pub Med Web of Science Crossref Google Scholar.
  22. von Meyer A, Cadamuro J, Lippi G, Simundic AM. Call for more transparency in manufacturers declarations on serum indices: On behalf of the Working Group for Preanalytical Phase (WG-PRE), European Federation of Clinical Chemistry and Laboratory Medicine (EFLM). Clin Chim Acta. 2018;484:328-332. Crossref Web of Science Google Scholar.
  23. Lippi G, Cadamuro J, von Meyer A, Simundic AM. European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for Preanalytical Phase (WG-PRE). Practical recommendations for managing hemolyzed samples in clinical chemistry testing. Clin Chem Lab Med. 2018;56:718-727. Crossref Google Scholar.
  24. Lippi G, Cadamuro J, von Meyer A, Simundic AM, European Federation of Clinical Chemistry, Laboratory Medicine (EFLM) Working Group; for Preanalytical Phase (WG-PRE). Local quality assurance of serum or plasma (HIL) indices. Clin Biochem. 2018;54:112-118. Crossref Google Scholar.
  25. Gómez Rioja R, Martínez Espartosa D, Segovia M, Ibarz M, Llopis MA, Bauça JM, et al. Laboratory sample stability. Is it possible to define a consensus stability function? An example of five blood magnitudes. Clin Chem Lab Med. 2018;56:1806-1818. Crossref Pub Med Web of ScienceGoogle Scholar.
  26. Oddoze C, Lombard E, Portugal H. Stability study of 81 analytes in human whole blood, in serum and in plasma. Clin Biochem. 2012;45:464-469. Pub Med Web of Science Crossref Google Scholar.
  27. STARD guidelines. Accessed 19 Oct 2018. Available from: http://www.equator-network.org/reporting-guidelines/stard/
  28. Sciacovelli L, Panteghini M, Lippi G, Sumarac Z, Cadamuro J, Galoro CA, et al. Defining a roadmap for harmonizing quality indicators in Laboratory Medicine: a consensus statement on behalf of the IFCC Working Group «Laboratory Error and Patient Safety» and EFLM Task and Finish Group «Performance specifications for the extra-analytical phases». Clin Chem Lab Med. 2017;55:1478-1488. Web of Science Google Scholar.
  29. Plebani M, Sciacovelli L, Aita A, Pelloso M, Chiozza ML. Performance criteria and quality indicators for the pre-analytical phase. Clin Chem Lab Med. 2015;53:943-948. Pub Med Web of Science Google Scholar.
  30. Plebani M, Sciacovelli L, Aita A. Quality indicators for the total testing process. Clin Lab Med. 2017;37:187-205. Web of Science Pub Med Crossref Google Scholar.
  31. Grebe SK, Singh RJ. LC-MS/MS in the clinical laboratory — where to from here? Clin Biochem Rev. 2011;32:5-31. Pub Med Google Scholar.
  32. Salvagno GL, Danese E, Lippi G. Preanalytical variables for liquid chromatography-mass spectrometry (LC-MS) analysis of human blood specimens. Clin Biochem. 2017;50:582-586. Crossref Web of Science Pub Med Google Scholar.
  33. Yin P, Lehmann R, Xu G. Effects of pre-analytical processes on blood samples used in metabolomics studies. Anal Bioanal Chem. 2015;407:4879-4892. Pub Med Crossref Web of Science Google Scholar.
  34. Heitzer E, Haque IS, Roberts CE, Speicher MR. Current and future perspectives of liquid biopsies in genomics-driven oncology. Nat Rev Genet. 2018. [Epub ahead of print]. Crossref Web of Science Google Scholar.
  35. Jung K, Fleischhacker M, Rabien A. Cell-free DNA in the blood as a solid tumor biomarker — a critical appraisal of the literature. Clin Chim Acta. 2010;411:1611-1624. Crossref Web of Science Google Scholar.
  36. Schmidt B, Reinicke D, Reindl I, Bork I, Wollschläger B, Lambrecht N, et al. Liquid biopsy — Performance of the PAXgene® blood ccfDNA tubes for the isolation and characterization of cell-free plasma DNA from tumor patients. Clin Chim Acta. 2017;469:94-98. Web of Science Crossref Pub Med Google Scholar.
  37. Betsou F. Biospecimen processing method validation. Biopreserv Biobank. 2015;13:69. Web of Science Pub Med Crossref Google Scholar.
  38. Gaignaux A, Ashton G, Coppola D, De Souza Y, De Wilde A, Eliason J, et al. A biospecimen proficiency testing program for biobank accreditation: four years of experience. Biopreserv Biobank. 2016;14:429-439. Web of Science Crossref Pub Med Google Scholar.
  39. Betsou F, Bulla A, Cho SY, Clements J, Chuaqui R, Coppola D, et al. Assays for qualification and quality stratification of clinical biospecimens used in research. Biopreserv Biobank. 2016;14:398-409. Web of Science Crossref Pub Med Google Scholar.
  40. Lundberg GD. Managing the patient-focused laboratory. Oradell, NJ: Medical Economics Co; 1975. Google Scholar.
  41. Anderson NL, Anderson NG. The human plasma proteome: history, character, and diagnostic prospects. Mol Cell Proteomics. 2002;1:845-867. Pub Med Crossref Google Scholar.
  42. Zimmer JS, Dyckes DF, Bernlohr DA, Murphy RC. Fatty acid binding proteins stabilize leukotriene A4: competition with arachidonic acid but not other lipoxygenase products. J Lipid Res. 2004;45:2138-2144. Pub Med Crossref Google Scholar.
  43. Thelen MH, Huisman W. Harmonization of accreditation to ISO15189. Clin Chem Lab Med. 2018;56:1637-1643. Pub Med Crossref Web of Science Google Scholar.
  44. Antonelli G, Padoan A, Aita A, Sciacovelli L, Plebani M. Verification of examination procedures in clinical laboratory for imprecision, trueness and diagnostic accuracy according to ISO 15189:2012: a pragmatic approach. Clin Chem Lab Med. 2017;55:1501-1508. Pub Med Web of Science Google Scholar. Поступила Received Принята к печати Accepted

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