Drug addiction and its detection are currently the most important medical and social problems. One of the main targets in the formation of dependence on specific drugs is sympatic, dopaminergic system and the metabolism of serotonin. The aim of this work is to study the diagnostic value of determination of main metabolites of serotonin and catecholamines in daily urine of patients with drug dependence with withdrawal syndrome. Material and methods. The study included 27 patients with heroin addiction, 24 patients with amphetamine dependence, and 20 healthy volunteers (control group). In all groups was determined the concentration of vanillylmandelic, homovanillic and 5-hydroxyindoleacetic acid in daily urine by gas chromatography — mass spectrometry. Results. Patients with heroin addiction at the stage of withdrawal had a statistically significant increase in the concentrations of all metabolites more than 2-fold compared to the control group. Patients with amphetamine dependence in the stage of withdrawal had a statistically significant increase in the concentrations of all metabolites more than 1.3 times compared to the control group. The variation in concentrations of vanillylmandelic and 5-hydroxyindoleacetic acid were within reference limits. Conclusions. In patients with narcotic dependence with withdrawal syndrome are greatly increased levels of metabolites of catecholamines and serotonin due to their increased synthesis. Increasing of the concentration of metabolites of catecholamines and serotonin can be used for early diagnosis of heroin dependence, and increase homovanillic acid on the background reference concentrations of other metabolites can be used for early diagnosis of amphetamine addiction in patients with withdrawal syndrome.