Gender and age affiliation are important factors that determine the intensity of metabolic processes. Among the risk factors for cardiovascular diseases special role belongs to metabolic factors, which primarily include the levels of glycemia and cholesterolemia. Gender and age affiliation are important factors that determine the intensity of metabolic processes. Among the risk factors for cardiovascular diseases special role belongs to metabolic factors, which primarily include the levels of glycemia and cholesterolemia. Aim: To estimate the age-related changes in blood glucose and cholesterol — metabolic factors of cardiovascular risk in men and women surveyed in accordance with the program of clinical examination. Material and methods. The study included people of both sexes (n=2261) aged 21 to 70 years of age and older who are at the dispensary. Patients were divided into 6 age groups. Determination of serum glucose and total cholesterol (TC) was performed using test systems company «Thermo Fisher Sientific» (Finland). Results. An increase in the studied biochemical parameters, the distinguished men and women in different age periods. By linear regression analysis of the correlation established age-glucose level with the presence of hypertension, diabetes. By eliminating the modifying effect of gender and age of the link between smoking/diabetes and cholesterol levels were found. Conclusion. Biochemical markers homeostasis (hyperglycemia and hypercholesterolemia) are, on the one hand, the reflection characteristics of age, and on the other element are triggering many pathological mechanisms, including diseases of the cardiovascular system. Watching the faces of all age groups, dispensary, it is important to maintain the level of glucose and cholesterol levels, focusing not on age but on the biological norm.