Evaluation of whole blood immunoreactivity

Seledtsova I.A.; Lyubavskaya T.Ya.; Pyshenko A.A.; Seledtsov V.I.

doi:https://doi.org/10.17116/hirurgia2024122122

Seledtsova I.A.

Petrovsky National Research Center of Surgery

ORCID: 0009-0006-0401-1876

Lyubavskaya T.Ya.

Petrovsky National Research Center of Surgery

ORCID: 0009-0002-8106-8148

Pyshenko A.A.

Petrovsky National Research Center of Surgery

ORCID: 0009-0002-1117-608X

Seledtsov V.I.

Petrovsky National Research Center of Surgery

ORCID: 0000-0002-4746-8853

Evaluation of whole blood immunoreactivity

Authors:

Seledtsova I.A., Lyubavskaya T.Ya., Pyshenko A.A., Seledtsov V.I.

More about the authors

Journal: Pirogov Russian Journal of Surgery. 2024;(12‑2): 122‑126

DOI: 10.17116/hirurgia2024122122

Read: 895 times

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To cite this article:

Seledtsova IA, Lyubavskaya TYa, Pyshenko AA, Seledtsov VI. Evaluation of whole blood immunoreactivity. Pirogov Russian Journal of Surgery. 2024;(12‑2):122‑126. (In Russ.)
https://doi.org/10.17116/hirurgia2024122122

Подписка 2026 Хирургия. Журнал им. Н.И. Пирогова (Pirogov Russian Journal of Surgery)

Использование инфографики авторами научных работ

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Abstract:

To assess cellular immunoreactivity, lipopolysaccharide (LPS), Concanavalin A (Con A), or LPS together with Con A was added to the whole blood for 18 hours. LPS preferentially stimulated release of tumor necrosis factor-alfa (TNF-α), interleukin-6 (IL-6), IL-10 and vascular endothelial growth factor (VEGF) by blood cells, whereas Con A significantly enhanced secretion of interferon-gamma (IFN-gamma) and IL-2. Addition of heparin to blood slightly decreased cellular secretion of IL-2 and VEGF, but not other cytokines. This test can be used to assess innate and adaptive blood immunoreactivity and may be useful in surgical practice for preparation of blood components (serum, plasma, leukocyte- and platelet-rich plasma) and objective immunologic monitoring.

Keywords:

whole blood immunoreactivity

heparin

vascular endothelium

Authors:

Seledtsova I.A.

Petrovsky National Research Center of Surgery

ORCID: 0009-0006-0401-1876

Lyubavskaya T.Ya.

Petrovsky National Research Center of Surgery

ORCID: 0009-0002-8106-8148

Pyshenko A.A.

Petrovsky National Research Center of Surgery

ORCID: 0009-0002-1117-608X

Seledtsov V.I.

Petrovsky National Research Center of Surgery

ORCID: 0000-0002-4746-8853

Received:

29.09.2024

Accepted:

16.10.2024

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References:

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