Routine methods of laboratory studies of intestinal microbiota: role and place in clinical practice

Kornoukhova L.A.

N.I.Pirogov Clinic of High Medical Technologies of the Saint-Petersburg State University;
Pavlov First Saint Petersburg State Medical University

SPIN RSCI: 5617-7827
ORCID: 0000-0002-3840-1032

Emanuel V.L.

N.I.Pirogov Clinic of High Medical Technologies of the Saint-Petersburg State University;
Pavlov First Saint Petersburg State Medical University

SPIN RSCI: 1177-4802
Scopus AuthorID: 6603693075
ORCID: 0000-0002-2079-0439

Denisov N.L.

N.I.Pirogov Clinic of High Medical Technologies of the Saint-Petersburg State University

RSCI AuthorID: 310235
Scopus AuthorID: 7006103472

Routine methods of laboratory studies of intestinal microbiota: role and place in clinical practice

Authors:

Kornoukhova L.A., Emanuel V.L., Denisov N.L.

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Journal: Russian Journal of Evidence-Based Gastroenterology. 2021;10(4): 5‑11

DOI: 10.17116/dokgastro2021100415

Downloaded: 159

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To cite this article:

Kornoukhova LA, Emanuel VL, Denisov NL. Routine methods of laboratory studies of intestinal microbiota: role and place in clinical practice. Russian Journal of Evidence-Based Gastroenterology. 2021;10(4):5‑11. (In Russ.)
https://doi.org/10.17116/dokgastro2021100415

Подписка 2025 (Russian Journal of Evidence-Based Gastroenterology)

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The intestinal microbiome studies received a powerful impetus associated with the development of laboratory technologies; however, the value of some diagnostic methods in clinical practice has not been established.

OBJECTIVE

Compare the results of two methods, the cultural and chromatographic.

MATERIAL AND METHODS

A comparative study of feces by chromatography-mass spectrometry of microbial metabolites/markers (MSMM) and bacteriological culture (BC) was carried out in 30 patients. Inclusion criteria: all studies were prescribed by a gastroenterologist; a one-time receipt of biomaterial for research. In BC, cultivation of facultatively anaerobic, obligate anaerobic microorganisms and fungi was performed. Microorganisms were identified by MALDI-TOF mass spectrometry. The MSMM study was carried out according to the author’s methodology of Prof. G.A. Osipov.

RESULTS

Intraspecies typing of anaerobes in BC allows to identify microecological changes in the intestine, assess bacterial properties and their changes, such as the acquisition of pathogenic factors. This is not available for MSMM, which determines 56 indicators represented by genera, species, and strains of microorganisms, complicating the assessment and interpretation of the results. The MSMM method lacks species differentiation of Lactobacillus spp. and Bifidobacterium spp., Enterobacteriaceae. The results of the methods do not correlate for Enterococcus spp. (77% of results), Enterobacteriaceae (60%), Staphylococcus spp. (67%), Lactobacillus spp. (30%), Bifidobacterium spp. and Bacteroides spp. — 17% of the results, respectively.

CONCLUSIONS

It is necessary to rank the informativeness of laboratory technologies and thoroughly prepare practitioners for using new laboratory methods in practice. At the moment, the chromatographic method MSMM can be used only for scientific purposes to determine microbial metabolites; for the quantitative assessment of microbial communities, validation and verification of the method is required.

Keywords:

fecal chromatography

mass spectrometry of microbial markers

Osipov method

intestinal dysbiosis

Authors:

Kornoukhova L.A.

N.I.Pirogov Clinic of High Medical Technologies of the Saint-Petersburg State University;
Pavlov First Saint Petersburg State Medical University

SPIN RSCI: 5617-7827
ORCID: 0000-0002-3840-1032

Emanuel V.L.

N.I.Pirogov Clinic of High Medical Technologies of the Saint-Petersburg State University;
Pavlov First Saint Petersburg State Medical University

SPIN RSCI: 1177-4802
Scopus AuthorID: 6603693075
ORCID: 0000-0002-2079-0439

Denisov N.L.

N.I.Pirogov Clinic of High Medical Technologies of the Saint-Petersburg State University

RSCI AuthorID: 310235
Scopus AuthorID: 7006103472

Received:

16.06.2021

Accepted:

21.10.2021

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