Ultrastructure of neointima of native and artificial elements of the blood circulatory system

Mukhamadiyarov R.A.; Koshelev V.A.; Frolov A.V.; Mironov A.V.; Shabaev A.R.; Evtushenko A.V.; Lyapin A.A.; Kutikhin A.G.

doi:https://doi.org/10.17116/patol20228403114

Mukhamadiyarov R.A.

Research Institute of Complex Problems of Cardiovascular Diseases

Koshelev V.A.

Research Institute for Complex Issues of Cardiovascular Diseases

Frolov A.V.

Research Institute for Complex Issues of Cardiovascular Diseases

Mironov A.V.

Academician Barbarash Kemerovo Regional Clinical Cardiology Dispensary

Shabaev A.R.

Research Institute for Complex Problems of Cardiovascular Diseases;
Barbarash Kuzbass Clinical Cardiology Dispensary

Evtushenko A.V.

Research Institute for Complex Issues of Cardiovascular Diseases

SPIN RSCI: 1085-0720
Scopus AuthorID: 7006834853
ORCID: 0000-0002-2955-5689

Lyapin A.A.

Research Institute for Complex Issues of Cardiovascular Diseases

Kutikhin A.G.

Research Institute of Complex Problems of Cardiovascular Diseases

Ultrastructure of neointima of native and artificial elements of the blood circulatory system

Authors:

Mukhamadiyarov R.A., Koshelev V.A., Frolov A.V., Mironov A.V., Shabaev A.R., Evtushenko A.V., Lyapin A.A., Kutikhin A.G.

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Journal: Russian Journal of Archive of Pathology. 2022;84(3): 14‑23

DOI: 10.17116/patol20228403114

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To cite this article:

Mukhamadiyarov RA, Koshelev VA, Frolov AV, et al. . Ultrastructure of neointima of native and artificial elements of the blood circulatory system. Russian Journal of Archive of Pathology. 2022;84(3):14‑23. (In Russ.)
https://doi.org/10.17116/patol20228403114

Подписка 2026 Архив патологии (Russian Journal of Archive of Pathology)

Использование инфографики авторами научных работ

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Abstract:

OBJECTIVE

To compare the neointima structure in conduits for coronary bypass grafting, bioprosthetic heart valves, tissue-engineered vascular grafts, and metal stents.

MATERIAL AND METHODS

The objects of the study were the fragments of the human internal thoracic artery, experimental biodegradable vascular prostheses, leaflets of xenopericardial bioprostheses of heart valves, and fragments of stented vessels. Tissue samples were fixed in formalin and post-fixed in osmium tetroxide. After dehydration and epoxy resin embedding, the samples were ground and polished. Samples were counterstained with uranyl acetate and lead citrate and visualized by means of backscattered scanning electron microscopy.

RESULTS

Neointimal pattern in all samples was similar. Neointima was comprised of endothelial cells, smooth muscle cells, fibroblasts, and the extracellular matrix. Endothelial cells showed significant diversity both between different elements of the circulatory system and within the same tissue, having either elongated or polygonal shape. Adhesion of leukocytes testified to the endothelial cell activation. In the absence of inflammation in the superficial layer of the neointima, the arrangement of smooth muscle cells and extracellular matrix fibers was parallel to the endothelium. Clusters of foam cells were frequently detected around the neointimal layers with solid inclusions (metal stents or calcium deposits). Thickening of the neointima was accompanied by the presence of capillaries and capillary-like structures.

CONCLUSION

Neointima formation is a typical response to the damage inflicted to the elements of the circulatory system. Neointima underwent a constant remodeling characterized by an altered cellular composition, macrophage invasion, neovascularization, and calcification.

Keywords:

neointima ultrastructure

calcium deposits

metal stents

vascular prostheses

superior thoracic artery

heart valves

Authors:

Mukhamadiyarov R.A.

Research Institute of Complex Problems of Cardiovascular Diseases

Koshelev V.A.

Research Institute for Complex Issues of Cardiovascular Diseases

Frolov A.V.

Research Institute for Complex Issues of Cardiovascular Diseases

Mironov A.V.

Academician Barbarash Kemerovo Regional Clinical Cardiology Dispensary

Shabaev A.R.

Research Institute for Complex Problems of Cardiovascular Diseases;
Barbarash Kuzbass Clinical Cardiology Dispensary

Evtushenko A.V.

Research Institute for Complex Issues of Cardiovascular Diseases

SPIN RSCI: 1085-0720
Scopus AuthorID: 7006834853
ORCID: 0000-0002-2955-5689

Lyapin A.A.

Research Institute for Complex Issues of Cardiovascular Diseases

Kutikhin A.G.

Research Institute of Complex Problems of Cardiovascular Diseases

Received:

09.01.2022

Accepted:

10.03.2022

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