Comparative assessment of the expression of Muc 2, Muc 5AC, and Muc 6 in serrated neoplasms of the colon

Mikhaleva L.M.

Research Institute of Human Morphology, Moscow, Russia

Vandysheva R.A.

Research Institute of Human Morphology, Moscow, Russia

ORCID: 0000-0002-9253-3044

Shakhpazyan N.K.

City Clinical Hospital Thirty-One, Moscow Healthcare Department, Moscow, Russia

Fedorov E.D.

Kafedra gospital'noĭ khirurgii #2 i nauchno-issledovatel'skaia laboratoriia khirurgicheskoĭ gastronterologii i ndoskopii Rossiĭskogo gosudarstvennogo meditsinskogo universiteta im. N.I. Pirogova

SPIN RSCI: 6785-9959
Scopus AuthorID: 7004381289
ORCID: 0000-0002-6036-7061

Biryukov A.E.

Municipal clinical hospital №31, Moscow

Midiber K.Yu.

City Clinical Hospital Thirty-One, Moscow Healthcare Department, Moscow, Russia

Pechnikova V.V.

Pirogov Russian National Research Medical University, Moscow, Russia

Comparative assessment of the expression of Muc 2, Muc 5AC, and Muc 6 in serrated neoplasms of the colon

Authors:

Mikhaleva L.M., Vandysheva R.A., Shakhpazyan N.K., Fedorov E.D., Biryukov A.E., Midiber K.Yu., Pechnikova V.V.

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Journal: Russian Journal of Archive of Pathology. 2019;81(2): 10‑17

DOI: 10.17116/patol20198102110

Downloaded: 55

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To cite this article:

Mikhaleva LM, Vandysheva RA, Shakhpazyan NK, Fedorov ED, Biryukov AE, Midiber KYu, Pechnikova VV. Comparative assessment of the expression of Muc 2, Muc 5AC, and Muc 6 in serrated neoplasms of the colon. Russian Journal of Archive of Pathology. 2019;81(2):10‑17. (In Russ.)
https://doi.org/10.17116/patol20198102110

Подписка 2025 (Russian Journal of Archive of Pathology)

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In the course of the serrated pathway of carcinogenesis, there are changes in the expression of mucins with a characteristic immunophenotypic sign, such as a late loss of intestinal differentiation and an increase in gastric differentiation. Objective — to comparatively assess the expression of Muc 2, Muc 5AC, and Muc 6 in hyperplastic polyps (HPs), sessile serrated adenomas (SSAs) and traditional serrated adenomas (TSAs) of the colon for determination of their role in differential diagnosis. Material and methods. Sixty-five serrated masses from 52 patients were examined. Among them, there were 26 SSAs, 26 HPs, and 13 TSAs. A histological examination was done using hematoxylin and eosin staining; periodic acid-Schiff reaction in combination with alcian blue, as well as immunohistochemistry with anti-Muc 2, anti-Muc 5AC, and anti-Muc 6 antibodies were used. Genetic testing of the specimens for KRAS and BRAF mutations was also carried out. Results. All the serrated neoplasms of the colon exhibited a pronounced expression of Muc 2. A marked Muc 6 expression in the dilated crypt bases was found in 76.9% of SSAs, while no reaction was seen in 92.3% of HPs and in 100% of TSAs. SSAs were characterized by an intense Muc 5AC expression in the whole length of the crypts and in the surface epithelium in contrast with HPs and TSAs, where the expression of the marker was focal. Comparison of the response of the markers and the presence of gene mutations identified that the SSAs with BRAF mutation intensely expressed along the length of the crypt for Muc 5AC and Muc 6; and the TSAs with KRAS mutation had a moderate focal Muc 5AC expression in the crypt bases in 100% of cases. Conclusion. For differential diagnosis of the types of serrated adenomas of the colon, it is useful for a pathologist to apply the immunohistochemical markers Muc 2, Muc 5AC, and Muc 6 in his/her practice.

Keywords:

serrated adenomas of the colon

sessile serrated adenoma

hyperplastic polyp

traditional serrated adenoma

Muc 2

Muc 6

Muc 5AC

KRAS

BRAF

Authors:

Mikhaleva L.M.

Research Institute of Human Morphology, Moscow, Russia

Vandysheva R.A.

Research Institute of Human Morphology, Moscow, Russia

ORCID: 0000-0002-9253-3044

Shakhpazyan N.K.

City Clinical Hospital Thirty-One, Moscow Healthcare Department, Moscow, Russia

Fedorov E.D.

Kafedra gospital'noĭ khirurgii #2 i nauchno-issledovatel'skaia laboratoriia khirurgicheskoĭ gastronterologii i ndoskopii Rossiĭskogo gosudarstvennogo meditsinskogo universiteta im. N.I. Pirogova

SPIN RSCI: 6785-9959
Scopus AuthorID: 7004381289
ORCID: 0000-0002-6036-7061

Biryukov A.E.

Municipal clinical hospital №31, Moscow

Midiber K.Yu.

City Clinical Hospital Thirty-One, Moscow Healthcare Department, Moscow, Russia

Pechnikova V.V.

Pirogov Russian National Research Medical University, Moscow, Russia

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