Adsorption capabilities of various extracorporeal devices for blood purification in increasing concentration of lipopolysaccharide. (In vitro benchmark experiment on fetal bovine serum)

Babaev M.A.

Petrovsky National Research Centre of Surgery

Dymova O.V.

Petrovsky National Research Centre of Surgery

Matveeva N.A.

Petrovsky National Research Centre of Surgery

Eremenko A.A.

Petrovsky National Research Center of Surgery;
Sechenov First Moscow State Medical University

ORCID: 0000-0001-5809-8563

Adsorption capabilities of various extracorporeal devices for blood purification in increasing concentration of lipopolysaccharide. (In vitro benchmark experiment on fetal bovine serum)

Authors:

Babaev M.A., Dymova O.V., Matveeva N.A., Eremenko A.A.

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Journal: Russian Journal of Anesthesiology and Reanimatology. 2021;(5): 40‑47

DOI: 10.17116/anaesthesiology202105140

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To cite this article:

Babaev MA, Dymova OV, Matveeva NA, Eremenko AA. Adsorption capabilities of various extracorporeal devices for blood purification in increasing concentration of lipopolysaccharide. (In vitro benchmark experiment on fetal bovine serum). Russian Journal of Anesthesiology and Reanimatology. 2021;(5):40‑47. (In Russ.)
https://doi.org/10.17116/anaesthesiology202105140

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OBJECTIVE

To evaluate the sorption characteristics of various extracorporeal devices in proportionally increasing concentration of lipopolysaccharide (LPS) in benchmark experimental model on fetal bovine serum.

MATERIAL AND METHODS

A bench experiment was carried out with fetal bovine serum circulating in a closed loop system at a temperature of 37 °C. LPS was injected in serum using a syringe pump at a constant rate of 33 μg per hour for 4 hours. We studied adsorption devices from 6 manufacturers differing in their design (granules, fiber, porous capillary), active adsorption ligand (polyethyleneimine, synthetic peptide, polymyxin B, etc.) and sorption capacities. Endotoxin level was determined using the LAL test before infusion, after 30, 60, 120 and 240 minutes. Sorption characteristics were evaluated using endotoxin adsorption coefficient (EAC).

RESULTS

All devices were able to bind LPS and corresponded to Langmuir isotherm model. However, EAC values differed significantly among the tested products. After 240 minutes, a product based on immobilized polymyxin B showed EAC 70.54%. A membrane of hollow fibers of acrylonitrile and methallyl sulfonate with heparin coating and sorbent based on polysaccharide granular matrix and chemical ligand specific to LPS adsorbed no more than 10.2% and 10.63%, respectively. Adsorption capacity of columns based on multimodal sorbent and device filled with porous polyethylene plates was 6.77 and 8.91%, respectively.

CONCLUSION

It is necessary to consider adsorption coefficient for maximum effect from selective extracorporeal therapy. In this experiment, a cartridge based on immobilized polymyxin B showed the maximum adsorption coefficient (70.54%). Products based on other principles of sorption and active ligands were characterized by adsorption coefficients 6.77—10.63%.

Keywords:

endotoxin

lipopolysaccharide adsorption

hemoperfusion

oXiris

LPS adsorber Alteco

Toxipak

Efferon LPS

Toraymyxin PMX-20R

Authors:

Babaev M.A.

Petrovsky National Research Centre of Surgery

Dymova O.V.

Petrovsky National Research Centre of Surgery

Matveeva N.A.

Petrovsky National Research Centre of Surgery

Eremenko A.A.

Petrovsky National Research Center of Surgery;
Sechenov First Moscow State Medical University

ORCID: 0000-0001-5809-8563

Received:

12.02.2021

Accepted:

23.03.2021

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