Current diagnostic methods in molecular classification of brain tumors at the Burdenko Neurosurgical Center

Ryzhova M.V.; Telysheva E.N.; Shaikhaev E.G.; Starovoitov D.V.; Kotelnikova A.O.; Galstyan S.A.; Okonechnikov K.V.

doi:https://doi.org/10.17116/neiro20218506198

Ryzhova M.V.

Burdenko Neurosurgical Center

ORCID: 0000-0001-7206-6365

Telysheva E.N.

Burdenko Neurosurgical Center

ORCID: 0000-0002-0370-8667

Shaikhaev E.G.

Burdenko Neurosurgical Center

ORCID: 0000-0002-7882-2579

Starovoitov D.V.

Burdenko National Medical Research Center of Neurosurgery

Kotelnikova A.O.

Burdenko Neurosurgical Center

Galstyan S.A.

Burdenko Neurosurgical Center

ORCID: 0000-0001-9953-6654

Okonechnikov K.V.

Hopp Children’s Cancer Center Heidelberg (KiTZ);
Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ)

Current diagnostic methods in molecular classification of brain tumors at the Burdenko Neurosurgical Center

Authors:

Ryzhova M.V., Telysheva E.N., Shaikhaev E.G., Starovoitov D.V., Kotelnikova A.O., Galstyan S.A., Okonechnikov K.V.

More about the authors

Journal: Burdenko's Journal of Neurosurgery. 2021;85(6): 98‑101

DOI: 10.17116/neiro20218506198

Read: 3017 times

To cite this article:

Ryzhova MV, Telysheva EN, Shaikhaev EG, Starovoitov DV, Kotelnikova AO, Galstyan SA, Okonechnikov KV. Current diagnostic methods in molecular classification of brain tumors at the Burdenko Neurosurgical Center. Burdenko's Journal of Neurosurgery. 2021;85(6):98‑101. (In Russ., In Engl.)
https://doi.org/10.17116/neiro20218506198

Подписка 2025 Журнал «Вопросы нейрохирургии» имени Н.Н. Бурденко (Burdenko's Journal of Neurosurgery)

Использование инфографики авторами научных работ

Close metadata

Recommended articles:

Origins of trigeminal neuralgia surgery (standing on the shoulders of giants). Burdenko's Journal of Neurosurgery. 2025;(5):103-108

Origins of trigeminal neuralgia surgery (era of microvascular decompression). Burdenko's Journal of Neurosurgery. 2025;(5):109-114

Modern understanding of epigenetic inheritance mechanisms and their role in the evolutionary process. Molecular Genetics, Microbiology and Virology. 2025;(4-2):6-20

Abstract:

DnA methylation has recently been accepted as the most reliable and effective method of diagnosing central nervous system (CNS) tumors. Healthy organs and tumors of different localizations have their own unique methylation structure. Determination of total tumor DNA methylome is the detection of all methylated nucleotides in a tumor. The "gold standard" for analyzing the methylation state of individual cytosines is bisulfite conversion, in which unmethylated cytosines are converted to uracils and read as thymines, while methylated cytosines are protected from conversion.

Keywords:

DNA methylation

EPIC array

Illumina Infinium HumanMethylation850 BeadChip

Authors:

Ryzhova M.V.

Burdenko Neurosurgical Center

ORCID: 0000-0001-7206-6365

Telysheva E.N.

Burdenko Neurosurgical Center

ORCID: 0000-0002-0370-8667

Shaikhaev E.G.

Burdenko Neurosurgical Center

ORCID: 0000-0002-7882-2579

Starovoitov D.V.

Burdenko National Medical Research Center of Neurosurgery

Kotelnikova A.O.

Burdenko Neurosurgical Center

Galstyan S.A.

Burdenko Neurosurgical Center

ORCID: 0000-0001-9953-6654

Okonechnikov K.V.

Hopp Children’s Cancer Center Heidelberg (KiTZ);
Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ)

Received:

02.03.2021

Accepted:

08.10.2021

Close metadata

References:

Capper D, Jones DTW, Sill M, Hovestadt V, Schrimpf D, Sturm D, Koelsche C, Sahm F, Chavez L, Reuss DE, Kratz A, Wefers AK, Huang K, Pajtler KW, Schweizer L, Stichel D, Olar A, Engel NW, Lindenberg K, Harter PN, Braczynski AK, Plate KH, Dohmen H, Garvalov BK, Coras R, Hölsken A, Hewer E, Bewerunge-Hudler M, Schick M, Fischer R, Beschorner R, Schittenhelm J, Staszewski O, Wani K, Varlet P, Pages M, Temming P, Lohmann D, Selt F, Witt H, Milde T, Witt O, Aronica E, Giangaspero F, Rushing E, Scheurlen W, Geisenberger C, Rodriguez FJ, Becker A, Preusser M, Haberler C, Bjerkvig R, Cryan J, Farrell M, Deckert M, Hench J, Frank S, Serrano J, Kannan K, Tsirigos A, Brück W, Hofer S, Brehmer S, Seiz-Rosenhagen M, Hänggi D, Hans V, Rozsnoki S, Hansford JR, Kohlhof P, Kristensen BW, Lechner M, Lopes B, Mawrin C, Ketter R, Kulozik A, Khatib Z, Heppner F, Koch A, Jouvet A, Keohane C, Mühleisen H, Mueller W, Pohl U, Prinz M, Benner A, Zapatka M, Gottardo NG, Driever PH, Kramm CM, Müller HL, Rutkowski S, von Hoff K, Frühwald MC, Gnekow A, Fleischhack G, Tippelt S, Calaminus G, Monoranu CM, Perry A, Jones C, Jacques TS, Radlwimmer B, Gessi M, Pietsch T, Schramm J, Schackert G, Westphal M, Reifenberger G, Wesseling P, Weller M, Collins VP, Blümcke I, Bendszus M, Debus J, Huang A, Jabado N, Northcott PA, Paulus W, Gajjar A, Robinson GW, Taylor MD, Jaunmuktane Z, Ryzhova M, Platten M, Unterberg A, Wick W, Karajannis MA, Mittelbronn M, Acker T, Hartmann C, Aldape K, Schüller U, Buslei R, Lichter P, Kool M, Herold-Mende C, Ellison DW, Hasselblatt M, Snuderl M, Brandner S, Korshunov A, von Deimling A, Pfister SM. DNA methylation-based classification of central nervous system tumours. Nature. 2018;555(7697):469-474. https://doi.org/10.1038/nature26000
Fernandez AF, Yassen Assenov Y, Martin-Subero JI, Balint B, Siebert R, Taniguchi H, Yamamoto H, Hidalgo M, Tan A-C, Galm O, Ferrer I, Sanchez-Cespedes M, Villanueva A, Carmona J, Sanchez-Mut JV, Berdasco M, Moreno V, Capella G, Monk D, Ballestar E, Ropero S, Martinez R, Sanchez-Carbayo M, Prosper F, Agirre X, Fraga MF, Grana O, Perez-Jurado L, Mora J, Puig S, Prat J, Badimon L, Puca AA, Meltzer S J, Lengauer T, Bridgewater J, Bock C,and Esteller M. A DNA methylation fingerprint of 1628 human samples. Genome Research. 2012;22(2):407-419. https://doi.org/10.1101/gr.119867.110
Capper D, Stichel D, Sahm F, Jones DTW, Schrimpf D, Sill M, Schmid S, Hovestadt V, Reuss DE, Koelsche C, Reinhardt A, Wefers AK, Huang K, Sievers P, Ebrahimi A, Schöler A, Teichmann D, Koch A, Hänggi D, Unterberg A, Platten M, Wick W, Witt O, Milde T, Korshunov A, Pfister SM, von Deimling A. Practical implementation of DNA methylation and copy-number-based CNS tumor diagnostics: the Heidelberg experience. Acta Neuropathologica. 2018;136(2):181-210. https://doi.org/10.1007/s00401-018-1879-y