Current diagnostic methods in molecular classification of brain tumors at the Burdenko Neurosurgical Center

Ryzhova M.V.

Burdenko Neurosurgical Center

ORCID: 0000-0001-7206-6365

Telysheva E.N.

Burdenko Neurosurgical Center

ORCID: 0000-0002-0370-8667

Shaikhaev E.G.

Burdenko Neurosurgical Center

ORCID: 0000-0002-7882-2579

Starovoitov D.V.

Burdenko National Medical Research Center of Neurosurgery

Kotelnikova A.O.

Burdenko Neurosurgical Center

Galstyan S.A.

Burdenko Neurosurgical Center

ORCID: 0000-0001-9953-6654

Okonechnikov K.V.

Hopp Children’s Cancer Center Heidelberg (KiTZ);
Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ)

Current diagnostic methods in molecular classification of brain tumors at the Burdenko Neurosurgical Center

Authors:

Ryzhova M.V., Telysheva E.N., Shaikhaev E.G., Starovoitov D.V., Kotelnikova A.O., Galstyan S.A., Okonechnikov K.V.

More about the authors

Journal: Burdenko's Journal of Neurosurgery. 2021;85(6): 98‑101

DOI: 10.17116/neiro20218506198

Downloaded: 87

To cite this article:

Ryzhova MV, Telysheva EN, Shaikhaev EG, Starovoitov DV, Kotelnikova AO, Galstyan SA, Okonechnikov KV. Current diagnostic methods in molecular classification of brain tumors at the Burdenko Neurosurgical Center. Burdenko's Journal of Neurosurgery. 2021;85(6):98‑101. (In Russ., In Engl.)
https://doi.org/10.17116/neiro20218506198

Подписка 2025-2 (Burdenko's Journal of Neurosurgery)

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DnA methylation has recently been accepted as the most reliable and effective method of diagnosing central nervous system (CNS) tumors. Healthy organs and tumors of different localizations have their own unique methylation structure. Determination of total tumor DNA methylome is the detection of all methylated nucleotides in a tumor. The "gold standard" for analyzing the methylation state of individual cytosines is bisulfite conversion, in which unmethylated cytosines are converted to uracils and read as thymines, while methylated cytosines are protected from conversion.

Keywords:

DNA methylation

EPIC array

Illumina Infinium HumanMethylation850 BeadChip

Authors:

Ryzhova M.V.

Burdenko Neurosurgical Center

ORCID: 0000-0001-7206-6365

Telysheva E.N.

Burdenko Neurosurgical Center

ORCID: 0000-0002-0370-8667

Shaikhaev E.G.

Burdenko Neurosurgical Center

ORCID: 0000-0002-7882-2579

Starovoitov D.V.

Burdenko National Medical Research Center of Neurosurgery

Kotelnikova A.O.

Burdenko Neurosurgical Center

Galstyan S.A.

Burdenko Neurosurgical Center

ORCID: 0000-0001-9953-6654

Okonechnikov K.V.

Hopp Children’s Cancer Center Heidelberg (KiTZ);
Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ)

Received:

02.03.2021

Accepted:

08.10.2021

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