The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-4329
+8 800 250-18-12
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2026
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Guzeev M.A.

Sechenov Institute of Evolutionary Physiology and Biochemistry

Kurmazov N.S.

Sechenov Institute of Evolutionary Physiology and Biochemistry

Simonova V.V.

Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences

Pastukhov Yu.F.

Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences

Ekimova I.V.

Sechenov Institute of Evolutionary Physiology and Biochemistry

Modeling of chronic sleep restriction for translational studies

Authors:

Guzeev M.A., Kurmazov N.S., Simonova V.V., Pastukhov Yu.F., Ekimova I.V.

More about the authors

Journal: S.S. Korsakov Journal of Neurology and Psychiatry. 2021;121(4‑2): 6‑13

DOI: 10.17116/jnevro20211214026

Read: 2592 times

Download PDF (RU)
Contact the author
Table of contents

MODELING OF CHRONIC SLEEP RESTRICTION FOR TRANSLATIONAL STUDIES
MODELING OF CHRONIC SLEEP RESTRICTION FOR TRANSLATIONAL STUDIES

To cite this article:

Guzeev MA, Kurmazov NS, Simonova VV, Pastukhov YuF, Ekimova IV. Modeling of chronic sleep restriction for translational studies. S.S. Korsakov Journal of Neurology and Psychiatry. 2021;121(4‑2):6‑13. (In Russ.)
https://doi.org/10.17116/jnevro20211214026

Подписка 2026 Журнал неврологии и психиатрии им. С.С. Корсакова. Спецвыпуски (S.S. Korsakov Journal of Neurology and Psychiatry (special issues))
МСФ на ЯМ
Использование инфографики авторами научных работ
Close metadata
Abstract:

OBJECTIVE

To develop of a chronic sleep restriction model in rats by repeated sleep deprivation using an orbital shaker and to determine whether this model leads to disturbances in sleep homeostatic mechanisms.

MATERIAL AND METHODS

Male Wistar rats (7—8 months old) underwent sleep restriction for five consecutive days: 3 h of sleep deprivation and 1 h of sleep opportunity repeating throughout each day. Polysomnograms were recorded telemetrically throughout the day before sleep restriction (baseline), on the 1st, 3rd, 5th day of sleep restriction and 2 days after the end of sleep restriction (recovery period).

RESULTS

During the period of sleep restriction, the total amount of slow-wave sleep (SWS) and rapid eye movement (REM) sleep decreased by 61% and 55%, respectively, compared to baseline. On the first day of recovery, amount of SWS increased mainly in the dark (active) phase of the day, while REM sleep increased in both light and dark phases; there was no marked rebound of daily SWS amount, while REM sleep increased by 30% from baseline. On the first day of recovery, an elevation of EEG beta and sigma power in sleep states was observed mainly in the light phase of the day. The loss of deep SWS throughout the sleep restriction period increased from 50% on 1st day to 75% on 5th day. The level of deep SWS remained below the baseline by 15—20% on the two subsequent days of recovery. The findings suggest that homeostatic mechanisms of SWS are persistently impaired after 5-day chronic sleep restriction. Besides, a decline of wakefulness accompanied by an increase of SWS in the active phase of the recovery period indicates a disruption in circadian rhythm.

CONCLUSION

The proposed model leads to the disruption of sleep homeostatic mechanisms, which, in turn, impede compensation of SWS loss caused by chronic insufficient sleep.

Keywords:

chronic sleep restriction
sleep homeostasis
deep sleep
circadian rhythm

Authors:

Guzeev M.A.

Sechenov Institute of Evolutionary Physiology and Biochemistry

Kurmazov N.S.

Sechenov Institute of Evolutionary Physiology and Biochemistry

Simonova V.V.

Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences

Pastukhov Yu.F.

Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences

Ekimova I.V.

Sechenov Institute of Evolutionary Physiology and Biochemistry

Received:

26.02.2021

Accepted:

05.03.2021

Close metadata

References:

  1. Poluektov M. Zagadki sna: Ot bessonnicy do letargii. M.: Al’pina Pablisher; 2019. (In Russ).
  2. Dorokhov VB. Somnology and Occupational Safety. Zhurnal VND. 2013;63(1):33-47. (In Russ.). https://doi.org/10.7868/S0044467713010048
  3. Lowe CJ, Safati A, Hall PA. The neurocognitive consequences of sleep restriction: a meta-analytic review. Neurosci Biobehav Rev. 2017;80:586-604.  https://doi.org/10.1016/j.neubiorev.2017.07.010
  4. Grandner MA, Sands-Lincoln MR, Pak VM, Garland SN. Sleep duration, cardiovascular disease, and proinflammatory biomarkers. Nat Sci Sleep. 2013;5:93.  https://doi.org/10.2147/NSS.S31063
  5. Centeradze SL, Poluektov MG. Rasstrojstva sna pri zabolevaniyah nervnoj sistemy. Medicinskij Sovet. 2018;1:46-50. (In Russ.). https://doi.org/10.21518/2079-701X-2018-1-46-50
  6. Isaev RI, Yakhno NN. Sleep disorders in Alzheimer’s disease. Nevrologicheskiy Zhurnal. 2017;22(5):228-236. (In Russ.).
  7. Beccuti G, Pannain S. Sleep and obesity. Curr Opin Clin Nutr Metab Care. 2011;14(4):402.  https://doi.org/10.1097/MCO.0b013e3283479109
  8. Reutrakul S, Van Cauter E. Sleep influences on obesity, insulin resistance, and risk of type 2 diabetes. Metabolism. 2018;84:56-66.  https://doi.org/10.1016/j.metabol.2018.02.010
  9. Kim Y, Laposky AD, Bergmann BM, Turek FW. Repeated sleep restriction in rats leads to homeostatic and allostatic responses during recovery sleep. Proc Natl Acad Sci USA. 2007;104(25):10697-10702. https://doi.org/10.1073/pnas.0610351104
  10. Kim Y, Bolortuya Y, Chen L, Basheer R, McCarley RW, Strecker RE. Decoupling of sleepiness from sleep time and intensity during chronic sleep restriction: evidence for a role of the adenosine system. Sleep. 2012;35(6):861-869.  https://doi.org/10.5665/sleep.1890
  11. Deurveilher S, Rusak B, Semba K. Time-of-day modulation of homeostatic and allostatic sleep responses to chronic sleep restriction in rats. Am J Physiol Regul Integr Comp Physiol. 2012;302(12):R1411-R1425. https://doi.org/10.1152/ajpregu.00678.2011
  12. Clasadonte J, McIver SR, Schmitt LI, Halassa MM, Haydon PG. Chronic sleep restriction disrupts sleep homeostasis and behavioral sensitivity to alcohol by reducing the extracellular accumulation of adenosine. J Neurosci. 2014;34(5):1879-1891. https://doi.org/10.1523/JNEUROSCI.2870-12.2014
  13. Kim B, Hwang E, Strecker RE, Choi JH, Kim Y. (2020). Differential modulation of NREM sleep regulation and EEG topography by chronic sleep restriction in mice. Sci Rep. 2020;10(1):1-13.  https://doi.org/10.1038/s41598-019-54790-y
  14. Leemburg S, Vyazovskiy VV, Olcese U, Bassetti CL, Tononi G, Cirelli C. Sleep homeostasis in the rat is preserved during chronic sleep restriction. Proc Natl Acad Sci USA. 2010;107(36):15939-15944. https://doi.org/10.1073/pnas.1002570107
  15. Simpson NS, Diolombi M, Scott-Sutherland J, Yang H, Bhatt V, Gautam S, Mullington J, Haack M. Repeating patterns of sleep restriction and recovery: do we get used to it? Brain Behav Immun. 2016;58:142-151.  https://doi.org/10.1016/j.bbi.2016.06.001
  16. Van Dongen H, Maislin G, Mullington JM, Dinges DF. The cumulative cost of additional wakefulness: dose-response effects on neurobehavioral functions and sleep physiology from chronic sleep restriction and total sleep deprivation. Sleep. 2003;26(2):117-126.  https://doi.org/10.1093/sleep/26.2.117
  17. Brodskij VYa, Nechaeva NV. Ritm sinteza belka. M.: Nauka; 1988. (In Russ.).
  18. Simonova VV, Guzeev MA, Pastukhov YuF. Sleep recovery after total sleep deprivation in conditions of prolonged deficiency of stress-inducible chaperone Hsp70. Russion Physiology Journal I.M. Sechenov. 2018;104(8):957-965. (In Russ.). https://doi.org/10.7868/S0869813918070055
  19. Maloney KJ, Cape EG, Gotman J, Jones BE. High-frequency γ electroencephalogram activity in association with sleep-wake states and spontaneous behaviors in the rat. Neuroscience. 1997;76(2):541-555.  https://doi.org/10.1016/S0306-4522(96)00298-9
  20. Banks S, Dinges DF. Behavioral and physiological consequences of sleep restriction. J Clin Sleep Med. 2007;3(5):519-528.  https://doi.org/10.5664/jcsm.26918
  21. Hall S, Deurveilher S, Ko KR, Burns J, Semba K. Region-specific increases in FosB/ΔFosB immunoreactivity in the rat brain in response to chronic sleep restriction. Behav Brain Res. 2017;322 (Pt A):9-17.  https://doi.org/10.1016/j.bbr.2017.01.024
  22. Wallingford JK, Deurveilher S, Currie RW, Fawcett JP, Semba K. Increases in mature brain-derived neurotrophic factor protein in the frontal cortex and basal forebrain during chronic sleep restriction in rats: possible role in initiating allostatic adaptation. Neuroscience. 2014;277:174-183.  https://doi.org/10.1016/j.neuroscience.2014.06.067
  23. Rechtschaffen A, Bergmann BM, Gilliland MA, Bauer K. Effects of method, duration, and sleep stage on rebounds from sleep deprivation in the rat. Sleep. 1999;22(1):11-31.  https://doi.org/10.1093/sleep/22.1.11
  24. Carskadon MA, Dement WC. Cumulative effects of sleep restriction on daytime sleepiness. Psychophysiology. 1981;18:107-113.  https://doi.org/10.1111/j.1469-8986.1981.tb02921.x
  25. Belenky G, Wesensten NJ, Thorne DR, Thomas ML, Sing HC, Redmond DP, Russo MB, Balkin TJ. Patterns of performance degradation and restoration during sleep restriction and subsequent recovery: a sleep dose-response study. J Sleep Res. 2003;12(1):1-12.  https://doi.org/10.1046/j.1365-2869.2003.00337.x
  26. Brunner DP, Dijk DJ, Borbely AA. Repeated partial sleep deprivation progressively changes in EEG during sleep and wakefulness. Sleep. 1993;16:100-113.  https://doi.org/10.1093/sleep/16.2.100
  27. Akerstedt T, Kecklund G, Ingre M, Lekander M, Axelsson J. Sleep homeostasis during repeated sleep restriction and recovery: Support from EEG dynamics. Sleep. 2009;32(2):217-222.  https://doi.org/10.5665/sleep/32.2.217
  28. Bjorness TE, Kelly CL, Gao T, Poffenberger V, Greene R W. Control and function of the homeostatic sleep response by adenosine A1 receptors. J Neurosci. 2009;29(5):1267-1276. https://doi.org/10.1523/JNEUROSCI.2942-08.2009
  29. Xie L, Kang H, Xu Q, Chen MJ, Liao Y, Thiyagarajan M, O’Donnell J, Christensen DJ, Nicholson C, Iliff JJ, Takano T, Deane R, Nedergaard M. Sleep drives metabolite clearance from the adult brain. Science. 2013;342(6156):373-377.  https://doi.org/10.1126/science.1241224
  30. Bonnet MH, Arand DL. Hyperarousal and insomnia: state of the science. Sleep Med Rev. 2010;14(1):9-15.  https://doi.org/10.1016/j.smrv.2009.05.002
  31. Nakanishi H, Sun Y, Nakamura RK, Mori K, Ito M, Suda S, Namba H, Storch FI, Dang TP, Mendelson W, Mishkin M. Positive correlations between cerebral protein synthesis rates and deep sleep in Macaca mulatta. Eur J Neurosci. 1997;9(2):271-279.  https://doi.org/10.1111/j.1460-9568.1997.tb01397.x
  32. Pastukhov YF, Simonova VV, Chernyshev MV, Guzeev MA, Shemyakova TS, Ekimova IV. Signs of sleep and behavior disorders indicating the initial stage of neurodegeneration in a rat model of Parkinson’s disease. Journal of Evolutionary Biochemistry and Physiology. 2017;53(5):431-434.  https://doi.org/10.1134/S0022093017050106
  33. Ekimova IV, Simonova VV, Guzeev MA, Lapshina KV, Chernyshev MV, Pastukhov YF. Changes in sleep characteristics of rat preclinical model of Parkinson’s disease based on attenuation of the ubiquitin—proteasome system activity in the brain. Journal of Evolutionary Biochemistry and Physiology. 2016;52(6):463-474.  https://doi.org/10.1134/S1234567816060057
Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.