The objective of the present study was to improve the quality and efficacy of the surgical treatment of chronic otitis media including the plastic correction of total and subtotal tympanic defects by fixing a many-layer chondro-perichondrial autograft with the use of a latex tissue glue. Twenty seven patients presenting with total and subtotal defects of the tympanic membrane underwent surgical intervention in the lateral position. The multilayer transplants composed of a thinned cartilage, fascia, and transposed circular cutaneous graft on the feeding pedicle were fixed using the latex tissue glue. The morphological and functional results of the treatment were estimated one year after surgery. It was shown that the use of the latex tissue glue for the fixation of many-layer autografts during myringoplasty made it possible to decrease the duration of the hospital stay of the patients, reduce to a minimum manifestations of negative subjective feelings, and ensure the reliable clinico-morphological and functional outcome of the treatment.