The aspects of a molecular-genetic study of human hair depending on their morphological characteristics. II. The peculiarities of genotyping

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Aleksandrova V.Yu.

Institute of Criminalistics, Centre for Special Techniques, Russian Federal Security Service, Moscow, Russia, 101000

Bogatyreva E.A.

Institute of Criminal Science, Centre of Special Technologies, Federal Security Service of the Russia, Moscow, Russia, 101000

Kuklev M.Iu.

Institut kriminalistiki Tsentra spetsial'noĭ tekhniki FSB Rossii, Moskva

Lapenkov M.I.

Institut kriminalistiki Tsentra spetsial'noĭ tekhniki FSB Rossii, Moskva

Plakhina N.V.

Institut kriminalistiki Tsentra spetsial'noĭ tekhniki FSB Rossii, Moskva

The aspects of a molecular-genetic study of human hair depending on their morphological characteristics. II. The peculiarities of genotyping

Authors:

Aleksandrova V.Yu., Bogatyreva E.A., Kuklev M.Iu., Lapenkov M.I., Plakhina N.V.

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Journal: Forensic Medical Expertise. 2019;62(2): 22‑25

DOI: 10.17116/sudmed20196202122

Views: 899

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To cite this article:

Aleksandrova VYu, Bogatyreva EA, Kuklev MIu, Lapenkov MI, Plakhina NV. The aspects of a molecular-genetic study of human hair depending on their morphological characteristics. II. The peculiarities of genotyping. Forensic Medical Expertise. 2019;62(2):22‑25. (In Russ.)
https://doi.org/10.17116/sudmed20196202122

Подписка 2025-2 (Forensic Medical Expertise)

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The objective of the present study was to optimize genotyping of nuclear DNA contained in human hair. The most efficient procedures for DNA isolation and typing are described taking into consideration the hair growth phase, the epithelial tissue conditions, and the number of nuclei in the near-root ends of the hair. The recommendations for the expert interpretation of the results of the molecular-genetic investigations are proposed.

Keywords:

human hair

telogen

prognostic screening

isolation of DNA

genotyping

Authors:

Aleksandrova V.Yu.

Institute of Criminalistics, Centre for Special Techniques, Russian Federal Security Service, Moscow, Russia, 101000

Bogatyreva E.A.

Institute of Criminal Science, Centre of Special Technologies, Federal Security Service of the Russia, Moscow, Russia, 101000

Kuklev M.Iu.

Institut kriminalistiki Tsentra spetsial'noĭ tekhniki FSB Rossii, Moskva

Lapenkov M.I.

Institut kriminalistiki Tsentra spetsial'noĭ tekhniki FSB Rossii, Moskva

Plakhina N.V.

Institut kriminalistiki Tsentra spetsial'noĭ tekhniki FSB Rossii, Moskva

List of references:

Aleksandrova VYu, Bogatyreva EA, Kuklev MYu, Lapenkov MI, Plahina NV. Molecular genetic aspects of people hair studies according to their morphological characteristics. I. Prediction screening. Sudebno-meditsinskaya ekspertiza. 2019;62(1):13-16. (In Russ.)
Gill P. Application of Low Copy Number DNA Profiling. Croatian Medical Journal. 2001;2(3):229-232.
Opel K, Fleishaker EL, Nicklas JA, Buel E, McCord BR. Evaluation and Quantification of Nuclear DNA from Human Telogen Hairs. Journal of Forensic Sciences. 2008;53(4):853-857. https://doi.org/10.1111/j.1556-4029.2008.00777
Seo SB, Ge J, King JL, Budowle B. Reduction of stutter ratios in short tandem repeat loci typing of low copy number DNA samples. Forensic Science International: Genetics. 2014;8:213-218. https://doi.org/10.1016/j.fsigen.2013.10.004
Smith PJ, Ballantyne J. Simplified low-copy-number DNA analysis by post-PCR purification. Journal of Forensic Sciences. 2007;52(4):820-829. https://doi.org/10.1111/j.1556-4029.2007.00470.x
Roeder AD, Elsmore P, Greenhalgh M, McDonald A. Maximizing DNA profiling success from sub-optimal quantities of DNA: A staged approach. Forensic Science International: Genetics. 2009;3:128-137. https://doi.org/10.1016/j.fsigen.2008.12.004
Butler JM, Shen Y, McCord BR. The Development of reduced size STR amplicons as tools for analysis of degraded DNA. Journal of Forensic Sciences. 2003;48(5):1054-1064.
Lapenkov MI, Plakhina NV, Alekseev YaI, Varlamov DA. The development of a test-sistem for the quantitative and qualitative evaluation of DNA content in criminalistics objects by the real-time polymerase chain reaction. Sudebno-meditsinskaia ekspertiza. 2011;54(2):34-38. (In Russ.)

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