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Loban I.E.

Biuro sudebno-meditsinskoĭ ékspertizy Leningradskoĭ oblasti

Gorbacheva T.V.

Saint-Petersburg Bureau of Forensic Medical Expertise, Saint-Petersburg Health Committee, Saint-Petersburg, Russia, 195067

Bychkov V.A.

Saint-Petersburg state budgetary institution «Bureau of Forensic Medical Expertise», Saint-Petersburg, Russia, 195067

The chemical toxicological investigation of gamma-hydroxybutyric acid in biological objects and the interpretation of the results of the analysis

Authors:

Loban I.E., Gorbacheva T.V., Bychkov V.A.

More about the authors

Journal: Forensic Medical Expertise. 2018;61(5): 25‑30

DOI: 10.17116/sudmed20186105125

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THE CHEMICAL TOXICOLOGICAL INVESTIGATION OF GAMMA-HYDROXYBUTYRIC ACID IN BIOLOGICAL OBJECTS AND THE INTERPRETATION OF THE RESULTS OF THE ANALYSIS
THE CHEMICAL TOXICOLOGICAL INVESTIGATION OF GAMMA-HYDROXYBUTYRIC ACID IN BIOLOGICAL OBJECTS AND THE INTERPRETATION OF THE RESULTS OF THE ANALYSIS

To cite this article:

Loban IE, Gorbacheva TV, Bychkov VA. The chemical toxicological investigation of gamma-hydroxybutyric acid in biological objects and the interpretation of the results of the analysis. Forensic Medical Expertise. 2018;61(5):25‑30. (In Russ.)
https://doi.org/10.17116/sudmed20186105125

Подписка 2025-2 (Forensic Medical Expertise)
 
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The authors propose a method for the quantitative determination of gamma-hydroxybutyric acid (GHBA) in biological objects including biological fluids (blood, urine) and tissues with the use of gas chromatography with mass-selective detector. The samples for the analysis were prepared by liquid-liquid extraction by butyl acetate with subsequent derivatization using a N,O-Bis(trimethylsilyl)trifluoroacetamide/Trimethylchlorosilane mixture (BSTFA + 1%TMCS). The graded graphs were linear in the range of GHBA concentrations from 26.4 to 1321.6 mg/l. Coefficients of correlation for all the graphs were higher than 0.999. The threshold of detectability for GHBA in blood, urine, and internal organs was 10 mg/l, the quantification limit concentration 26 mg/l.

Keywords:

sodium hydroxybutyrate
GHBA
forensic medical investigations
gas chromatography with mass-selective detector (GC-MC)

Authors:

Loban I.E.

Biuro sudebno-meditsinskoĭ ékspertizy Leningradskoĭ oblasti

Gorbacheva T.V.

Saint-Petersburg Bureau of Forensic Medical Expertise, Saint-Petersburg Health Committee, Saint-Petersburg, Russia, 195067

Bychkov V.A.

Saint-Petersburg state budgetary institution «Bureau of Forensic Medical Expertise», Saint-Petersburg, Russia, 195067

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