Multilocus genotyping of polymorphous STR-loci of chromosomal DNA in individual cells: technical difficulties

Ivanov P.L.; Leonov S.N.; Zemskova E.Iu.; Kobylianskiĭ A.G.; Dziubenko E.V.

doi:https://doi.org/

Ivanov P.L.

FGBU "Rossiĭskiĭ tsentr sudebno-meditsinskoĭ ékspertizy" Minzdravsotsrazvitiia Rossii, Moskva

Leonov S.N.

FGBU "Rossiĭskiĭ tsentr sudebno-meditsinskoĭ ékspertizy" Minzdravsotsrazvitiia Rossii, Moskva

Zemskova E.Iu.

FGBU "Rossiĭskiĭ tsentr sudebno-meditsinskoĭ ékspertizy" Minzdravsotsrazvitiia Rossii, Moskva

Kobylianskiĭ A.G.

Institut molekuliarnoĭ genetiki Rossiĭskoĭ akademii nauk, Moskva

Dziubenko E.V.

Institut molekuliarnoĭ genetiki Rossiĭskoĭ akademii nauk, Moskva

Multilocus genotyping of polymorphous STR-loci of chromosomal DNA in individual cells: technical difficulties

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Ivanov P.L., Leonov S.N., Zemskova E.Iu., Kobylianskiĭ A.G., Dziubenko E.V.

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Journal: Forensic Medical Expertise. 2013;56(5): 19‑23

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Ivanov PL, Leonov SN, Zemskova EIu, Kobylianskiĭ AG, Dziubenko EV. Multilocus genotyping of polymorphous STR-loci of chromosomal DNA in individual cells: technical difficulties. Forensic Medical Expertise. 2013;56(5):19‑23. (In Russ.)

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

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Abstract:

This study was designed to estimate the effectiveness of special technical procedures for the enhancement of sensitivity of multiplex analysis of DNA, such as the use of low-plexity PCR systems and the whole genome preamplification technology, and the possibility of their application for the purpose of forensic medical genotyping of polymorphous STR-loci of chromosomal DNA in individual cells. The authors refused to use the imitation model (equivalent DNA dilutions) for the sake of obtaining the maximally informative data and chose to work with real preparations of solitary buccal epithelial cells isolated by the laser microdissection technique. It was shown that neither the use of the low-plexity multilocus PCR systems nor the whole genome pre-amplification technology makes possible reliable genotyping of STR-loci of chromosomal DNA in individual cells. The proposed techniques allow for DNA genotyping in preparations consisting of 10 diploid cells whereas the methods for reliable genotyping of STR-loci of chromosomal DNA in individual cells remains to be developed.

Keywords:

laser microdissection

molecular-genetic expertise

multiplex genotyping of STR-loci of chromosomal DNA in individual cells

Authors:

Ivanov P.L.

FGBU "Rossiĭskiĭ tsentr sudebno-meditsinskoĭ ékspertizy" Minzdravsotsrazvitiia Rossii, Moskva

Leonov S.N.

FGBU "Rossiĭskiĭ tsentr sudebno-meditsinskoĭ ékspertizy" Minzdravsotsrazvitiia Rossii, Moskva

Zemskova E.Iu.

FGBU "Rossiĭskiĭ tsentr sudebno-meditsinskoĭ ékspertizy" Minzdravsotsrazvitiia Rossii, Moskva

Kobylianskiĭ A.G.

Institut molekuliarnoĭ genetiki Rossiĭskoĭ akademii nauk, Moskva

Dziubenko E.V.

Institut molekuliarnoĭ genetiki Rossiĭskoĭ akademii nauk, Moskva

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References:

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