The study samples included 12 anatomical specimens taken from the fixed corpses of various gender and age. Pterygomandibular space topography was assessed by macro — and micropreparation methods. The landmarks for the universal way of mandibular anesthesia were defined associated with low risk of medial pterygoid muscle, lingual nerve, inferior alveolar neurovascular bundle damage. The study results allow increasing the efficiency and safety of anesthesia of the lower jaw teeth.