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Burlev V.A.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

Il'iasova N.A.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

Neuroangiogenesis in endometrium of women with endometriosis and chronic pelvic pain: high expression of vasoactive intestinal peptide

Authors:

Burlev V.A., Il'iasova N.A.

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Journal: Russian Journal of Human Reproduction. 2017;23(6): 71‑82

DOI: 10.17116/repro201723671-82

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NEUROANGIOGENESIS IN ENDOMETRIUM OF WOMEN WITH ENDOMETRIOSIS AND CHRONIC PELVIC PAIN: HIGH EXPRESSION OF VASOACTIVE INTESTINAL PEPTIDE
NEUROANGIOGENESIS IN ENDOMETRIUM OF WOMEN WITH ENDOMETRIOSIS AND CHRONIC PELVIC PAIN: HIGH EXPRESSION OF VASOACTIVE INTESTINAL PEPTIDE

To cite this article:

Burlev VA, Il'iasova NA. Neuroangiogenesis in endometrium of women with endometriosis and chronic pelvic pain: high expression of vasoactive intestinal peptide. Russian Journal of Human Reproduction. 2017;23(6):71‑82. (In Russ.)
https://doi.org/10.17116/repro201723671-82

 
Подписка 2026 Проблемы репродукции (Russian Journal of Human Reproduction)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

Objectives — to assess the intensity of expression of VIP and VEGF-A in the vessels, the proliferative index in the vessels and in the epithelium of glands, the density of CD31 and VIP in ectopic and eutopic endometrium in patients with peritoneal form of endometriosis without chronic pelvic pain and with pain, as well as study the content of VIP, VEGF -A, IL-6 in the peritoneal fluid and in the serum of the examined patients. Material and methods. Immunohistochemistry was used to study the expression of VIP, VEGF-A and microvessel density, Western blot for the analysis of VIP protein expression, polymerase chain reaction for the evaluation of mRNA VIP expression in ectopic and eutopic endometrium in women with endometriosis and normal. The ELISA method was used to determine the concentration of VIP, VEGF-A, IL-6 in the peritoneal fluid and in blood serum. Results. When comparing the VIP content (ELISA, enzyme immunoassay) in the peritoneal fluid and blood; expression VIP (IHC, immunohistochemistry), expression of the VIP protein (WB, western blotting), mRNA VIP expression (PCR, polymerase chain reaction) in ectopic and eutopic endometrium in patients with endometriosis and pelvic pain statistically significant upregulation of VIP was shown. Conclusion. The role of VIP in the formation of pain in endometriosis has been proved on transcriptomic and proteomic levels. Subsequent functional studies of the role of VIP in endometriosis will allow to establish or disprove the development on this basis of therapeutic or diagnostic capabilities.

Keywords:

endometriosis
pain
ectopic endometrium
eutopic endometrium
angiogenesis
neurogenesis

Authors:

Burlev V.A.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

Il'iasova N.A.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

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References:

  1. Burlev VA. Proliferativnaya i angiogennaya aktivnost’ eutopicheskogo i ektopicheskogo endometriya u bol’nykh s peritoneal’noi formoi endometrioza. Problemy reproduktsii. 2006;1:78-87. (In Russ.) Available at: http://elibrary.ru/item.asp?id=21947357. Accessed September 22, 2017
  2. Burlev VA. The inflammation stress: Systemic angiogenesis, acute phase proteins and tissue destruction products in patients with chronic recurrent salpingoophoritis. Problemy reproduktsii. 2011;5:25-32. (In Russ.) Available at: http://elibrary.ru/item.asp?id=18949299 Accessed September 22, 2017
  3. Burlev VA. Ectopic endometrium: proliferative activity and apoptosis in patients with peritoneal form of genital endometriosis. Problemy reproduktsii. 2012;6:7-13. (In Russ.) Available at: http://elibrary.ru/item.asp?id=18776994 Accessed September 22, 2017
  4. Burlev VA. Pelvic peritoneum and peritoneal adhesions: inflammatory stress. Problemy reproduktsii. 2015;1:15-25. (In Russ.) https://doi.org/10.17116/repro20152115-25
  5. Burlev VA, Ilyasova NA. Signaling LIF and IL-1 pathways changes in patients with peritoneal endometriosis and infertility. Problemy reproduktsii. 2015;21(4):99-109. (In Russ.) https://doi.org/10.17116/repro201521499-109
  6. Burlev VA, Ilyasova NA. Stress-limiting and stress-realizing systems in endometrium of endometriosis: effects of treatment on the expression of stress proteins and angiogenesis. Problemy reproduktsii. 2017;1:61-69. (In Russ.) https://doi.org/10.17116/repro201723161-69
  7. Burlev VA, Ilyasova NA. Angiogenic properties of endometrial aquaporins in patients with peritoneal endometriosis. Problemy reproduktsii. 2017;3:19-31. (In Russ.) https://doi.org/10.17116/repro201723319-31
  8. Burlev VA, Lec NI. Rol’ brjushiny v patogeneze naruzhno-genital’nogo jendometrioza (obzor literatury). Problemy reprodukcii. 2001;1:25-29. (In Russ.) Available at: http://www.rusmedserv.com/problreprod/2001g/1/article_729.html Accessed September 22, 2017
  9. Burlev VA, Pavlovich SV. Angiogenez v razvitii peritoneal’nogo jendometrioza. Problemy reprodukcii. 2003;2:42-47. (In Russ.) Available at: https://elibrary.ru/item.asp?id=24856345 Accessed September 22, 2017
  10. Burlev VA, Gasparov AS, Dubinskaya ED. Varianty raspredelenija geterotopij na brjushine malogo taza u bol’nyh s jendometriozom. Problemy reprodukcii. 2006;4:84-88. (In Russ.) Available at: http://www.fesmu.ru/elib/Article.aspx?id=151895 Accessed September 22, 2017
  11. Burlev VA, Il’iasova NA, Burlev AV, Dubinskaya ED. Pelvic and extrapelvic peritoneum: angiogenic activity and apoptosis in patients with endometriosis. Problemy reproduktsii. 2010;4:7-15. (In Russ.) https://elibrary.ru/item.asp?id=18965547 Accessed September 22, 2017
  12. Kogan EA, Ovakimyan AS, Paramonova NB, Faizullina NM, Kozachenko IF, Adamyan LV. Morphological substrate and pathogenetic mechanisms of pelvic pain syndrome in endometriosis. Part II. Peripheral nerve tissue remodeling in the foci of endometriosis. Arkhiv patologii. 2016;3:20-25. (In Russ.) https://doi.org/10.17116/patol201678320-25
  13. Kurbacheva OM, Pavlova KS. Phenotypes and endotypes of bronchial asthma: from pathogenesis and clinical features to therapy. Russian Allergology Journal. 2013;1:15-24. (In Russ.) Available at: http://rusalljournal.ru/en/journals/raj-for-2013/ Accessed September 22, 2017
  14. Editorial Article. Jendotipy i fenotipy astmy — ot algoritma obsledovanija do podbora terapii. Meditsinskiy Sovet. (Medical Council). 2015;(4):8-19. (In Russ.) https://doi.org/10.21518/2079-701X-2015-4-8-19
  15. Arosh JA, et al. Molecular and preclinical basis to inhibit PGE2 receptors EP2 and EP4 as a novel nonsteroidal therapy for endometriosis. Proc Natl Acad Sci USA. 2015;112(31):9716-9721. https://doi.org/10.1073/pnas.1507931112
  16. Bourlev V, Moberg C, Ilyasova N, Davey E, Kunovac Kallak Th, Olovsson M. Vasoactive intestinal peptide is upregulated in women with endometriosis and chronic pelvic pain. In thesis: the human endometrium, stadies on angiogenesis and endometriosis, 2016, identifiers URN: urn:nbn:se:uu:diva-314225 OAI: 2017 oai:DiVA.org:uu-314225 DiVA: diva2:1070106. Available at: http://uu.diva-portal.org/smash/record.jsf?pid=diva2%3A1070106&dswid=-3132 Accessed September 22, 2017
  17. Bourlev V, Volkov N, Pavlovitch S, Lets N, Larsson A, Olovsson M. The relationship between microvessel density, proliferative activity and expression of vascular endothelial growth factor-A and its receptors in eutopic endometrium and endometriotic lesions. Reproduction. 2006;132(3):501-509. https://doi.org/10.1530/rep.1.01110
  18. Danese S, Deban L. Does the mucosal microcirculation play a role in IBD? Inflamm Bowel Dis. 2008;14 Suppl 2:125-126. https://doi.org/10.1002/ibd.20716
  19. Defrère S, Van Langendonckt A, Ramos RG, Jouret M, Mettlen M, Donnez J. Quantification of endometriotic lesions in a murine model by fluorimetric and morphometric analyses. Human Reproduction. 2006;21:3:810-817. https://doi.org/10.1093/humrep/dei387
  20. Ellett L, Readman E, Newman M, McIlwaine K, Villegas R, Jagasia N, Maher P. Are endometrial nerve fibres unique to endometriosis? A prospective case-control study of endometrial biopsy as a diagnostic test for endometriosis in women with pelvic pain. Hum Reprod. 2015;30(12):2808-2815. https://doi.org/10.1093/humrep/dev259
  21. Giudice LC. Clinical practice. Endometriosis. N Engl J Med. 2010;362(25):2389-2398. https://doi.org/10.1056/NEJMcp1000274
  22. Greaves E, Collins F, Esnal-Zufiaurre A, Giakoumelou S, Horne AW, Saunders PT. Estrogen receptor (ER) agonists differentially regulate neuroangiogenesis in peritoneal endometriosis via the repellent factor SLIT3. Endocrinology. 2014;155(10):4015-4026. https://doi.org/10.1210/en.2014-1086
  23. Halme J, Hammond MG, Hulka J, Raj SG, Talbert LM. Retrograde menstruation in healthy women and in patients with endometriosis. Obst Gyn. 1984;64:151-154. Available at: https://www.ncbi.nlm.nih.gov/pubmed/6234483 Accessed September 22, 2017
  24. Khan KN, Masuzaki H, Fujishita A, Kitajima M, Sekine I, Ishimaru T. Higher activity by opaque endometriotic lesions than in nonopaque lesions. Acta Obstet Gynecol Scand. 2004;83:375-382. https://doi.org/10.1111/j.0001-6349.2004.00229.x
  25. Leyendecker G, Herbertz G, Kunz G, Mall G. Endometriosis results from the dislocation of basal endometrium. Hum Reprod. 2002;17(10):2725-2736. https://doi.org/10.1093/humrep/17.10.2725
  26. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T). Method Methods. 2001;25(4):402-408. https://doi.org/10.1006/meth.2001.1262
  27. Maharajaa Sri PK, Black KI, Manconi F, Ng CHM, Hey-Cunningham A, Fraser IS, Berbic M. An investigation of the relationship between pelvic pain and density of nerve fibers in peritoneal lesions of endometriosis. Journal of Endometriosis and Pelvic Pain Disorders. 2014;6(2):84-91. https://doi.org/10.5301/je.5000185
  28. Morotti M, Vincent K, Brawn J, Zondervan KT, Becker CM. Peripheral changes in endometriosis-associated pain. Hum Reprod Update. 2014;20(5):717-736. https://doi.org/10.1093/humupd/dmu021
  29. Newman TA, Bailey JL, Stocker LJ, Woo YL, Macklon NS, Cheong YC. Expression of neuronal markers in the endometrium of women with and those without endometriosis. Hum Reprod. 2013;28(9):2502-2510. https://doi.org/10.1093/humrep/det274
  30. Nisolle M, Casanas-Roux F, Anaf V, Mine JM, Donnez J. Morphometric study of the stromal vascularization in peritoneal endometriosis. Fertil Steril. 1993;59:681-684. https://doi.org/10.1016/S0015-0282(16)55800-2
  31. Nisoole M, Donnez J. Peritoneal endometriosis, ovarian endometriosis, and adenomyotic nodules of rectovaginal septum are three different entities. Fertil Steril. 1997;68(4):585-596. https://doi.org/10.1016/S0015-0282(97)00191-X
  32. Revised American Society for Reproductive Medicine classification of endometriosis: 1996. Fertil Steril. 1997;67:817-821. https://doi.org/10.1016/S0015-0282(97)81391-X
  33. Santanam N, Murphy AA, Parthasarathy S. Macrophages, oxidation, and endometriosis. Annals of the New York Academy of Sciences. 2002;955:183-198. https://doi.org/10.1111 / j.1749-6632.2002.tb02779.x
  34. Tokushige N, Markham R, Russell P, Fraser I.S. Nerve fibres in peritoneal endometriosis. Hum Reprod. 2006;21(11):3001-3007. https://doi.org/10.1093/humrep/del260
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