Tissue and molecular effects of the cavitated solution in the endometrial receptivity rehabilitation of patients with the uterine infertility

Melkozerova O.A.

Ural'skiĭ nauchno-issledovatel'skiĭ institut okhrany materinstva i mladenchestva, Ekaterinburg

Bashmakova N.V.

Ural Scientific Research Institute of Maternity and Child Health Ministry of the Russia, Yekaterinburg, Russia

Chistiakova G.N.

FGBU "Ural'skiĭ nauchno-issledovatel'skiĭ institut okhrany materinstva i mladenchestva", Ekaterinburg

Esareva A.V.

Ural Research Institute of Maternal and Infant Care, Ministry of Health of Russia, Yekaterinburg, Russia

Barlit O.G.

Urals Research Institute for Maternal and Child Care, Ministry of Health of Russia, Yekaterinburg, Russia

Giniyatova A.A.

Urals Research Institute for Maternal and Child Care, Ministry of Health of Russia, Yekaterinburg, Russia

Tissue and molecular effects of the cavitated solution in the endometrial receptivity rehabilitation of patients with the uterine infertility

Authors:

Melkozerova O.A., Bashmakova N.V., Chistiakova G.N., Esareva A.V., Barlit O.G., Giniyatova A.A.

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Journal: Russian Journal of Human Reproduction. 2017;23(5): 73‑83

DOI: 10.17116/repro201723573-83

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To cite this article:

Melkozerova OA, Bashmakova NV, Chistiakova GN, Esareva AV, Barlit OG, Giniyatova AA. Tissue and molecular effects of the cavitated solution in the endometrial receptivity rehabilitation of patients with the uterine infertility. Russian Journal of Human Reproduction. 2017;23(5):73‑83. (In Russ.)
https://doi.org/10.17116/repro201723573-83

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Objective — to evaluate the efficiency of granulocyte colony-stimulating factor (G-CSF) cavitated low-frequency sonication in restoring endometrial receptivity in patients with uterine infertility. Material and methods. 92 women with uterine infertility due to endometrial hypoplasia were examined in the dynamics of therapy including irrigation of the uterine cavity with cavitated low-frequency ultrasound with drug solutions. The control group consisted of 28 healthy fertile women. A histological and immunohistochemical study of the endometrium was performed on the «implantation window» LH + 7, in dynamics before the start of therapy and in the next cycle after the end of treatment. Results. After cavitation irrigation of the uterine cavity, 62 (67.39%) women with infertility in the endometrial specimens had adequate vascularization of the stroma (before treatment, 36.95%; p=0.017, in control 78.57%), in 68 (73, 91%) women — a decrease in the density of the stromal matrix, in 64 (69.56%) — according to light microscopy data, mature pinopodia were determined (initially 28.26%; p=0.0015). On the background of therapy, the ratio of ERα/PR expression in the stroma of the implantation endometrium was normalized, which was characterized by the prevalence of the PR pool (1.01±0.34 before treatment, 0.36±0.03 after therapy; p=0.040, in control 0.34±0.06; p>0.05). The parameters of stromal expression of natural uterine killers CD56 + bright against the background of treatment in patients with infertility come in accordance with the indices of healthy fertile women (from 35.21±2.14 to 45.75±3.18 cells l/s; in the control 47.8±2.13 cells in l/s; p>0.05). A significant decrease in stromal expression of CD3+ is noted: 78.17±6.89 cells in l/s versus 23.83±3.63 cells in l/s; p=0.004. The expression level of CD34 in the endometrial stroma after the treatment was significantly increased (20.88±0.77 cells in l/s versus 33.83±3.63 cells in l/s; p=0.035). Conclusion. The use of G-CSF cavitaion solutions for irrigation of the uterine cavity appears to be effective in restoring tissue and molecular endometrial receptivity in patients with uterine infertility due to endometrial hypoplasia.

Keywords:

uterine infertility

endometrial hypoplasia

cavitation irrigation of uterine cavity

granulocyte colony-stimulating factor

Authors:

Melkozerova O.A.

Ural'skiĭ nauchno-issledovatel'skiĭ institut okhrany materinstva i mladenchestva, Ekaterinburg

Bashmakova N.V.

Ural Scientific Research Institute of Maternity and Child Health Ministry of the Russia, Yekaterinburg, Russia

Chistiakova G.N.

FGBU "Ural'skiĭ nauchno-issledovatel'skiĭ institut okhrany materinstva i mladenchestva", Ekaterinburg

Esareva A.V.

Ural Research Institute of Maternal and Infant Care, Ministry of Health of Russia, Yekaterinburg, Russia

Barlit O.G.

Urals Research Institute for Maternal and Child Care, Ministry of Health of Russia, Yekaterinburg, Russia

Giniyatova A.A.

Urals Research Institute for Maternal and Child Care, Ministry of Health of Russia, Yekaterinburg, Russia

List of references:

The European health report 2015. Targets and beyond — reaching new frontiers in evidence. Copenhagen: WHO Regional Office for Europe, 2015. Accessed August 28, 2017. (In Russ.)
Health-2020: a European policy framework and strategy for the 21st century. Copenhagen: WHO Regional Office for Europe; 2013. http://www.euro.who.int/en/publications/abstracts/health-2020-a-european-policy-framework-and-strategy-for-the-21stcentury
Ermolenko KS, Radzinskij VE, Rapoport SI. The current state of the problem of the realization of the fertile function of women of late reproductive age. Klinicheskaya medicina. 2016;94(1):10-15. (In Russ.)
Register ART RAHR. Report for 2014. SPb; 2015. (In Russ.)
Gyul’mamedova ID, Mezhova OK. Problems of implantation in the IVF program. Novosti mediciny i farmacii. 2008;253:17-27. (In Russ.)
Boyarskij KYu, Gajdukov SN, Pal’chenko NA. Modern view on the problem of receptivity and thin endometrium in ART programs. Literature review. Problemy reprodukcii. 2013;4:3-7. (In Russ.) Accessed August 28, 2017. https://www.mediasphera.ru/issues/problemy-reproduktsii/2013/4/031025-72172013411
Uterine Endometrial Function . Editor of H. Kanzaki. Osaka: Springer; 2017.
Molecular mechanisms of the reproductive system diseases. Editor of Pal’cev MA. SPb.: Eko-vektor; 2017. (In Russ.)
Pregravid preparation: the clinical protocol. Ed. of Radzinskij VE. М.: Status Praesens; 2016;23-25. (In Russ.)
Крылова Ю.М., Кветной И.М., Айламазян Э.К. Рецептивность эндометрия: молекулярные механизмы регуляции имплантации. Журнал акушерства и женских болезней. 2013;2:63-74. [Krylova YuM, Kvetnoj IM, Ajlamazyan EhK. Receptivity of the endometrium: molecular mechanisms of regulation of implantation. Zhurnal akusherstva i zhenskih boleznej. 2013;2:63-74. https://doi.org/10.17816/JOWD663135-142
Li ZL, Ueki K, Kumagai K. Regulation of bcl-2 transcription by estrogen receptor-alpha and C-Jun in human endometrium. Med Mol Morphol. 2014;47(1):43-53. https://doi.10.1007/s00795-013-0043-y
Ramli R, Reher P, Harris M. The effect of ultrasound on angiogenesis: an in vivo study using the chick chorioallantoic membrane. Int J Oral Maxillofac Implants. 2009;24:591-596. PMID: 19885398
Barzelai S, Sharabani-Yosef O, Walden R. Low-intensity ultrasound induces angiogenesis in rat hind-limb ischemia. Ultrasound Med Biol. 2006;32:139-145. https://doi.org/10.1016/j.ultrasmedbio.2005.08.010
Suchkova VN, Baggs RVB. Ultasound improves tissue perfusion in ischemic tissue through a nitric oxide dependent mechanism. Thromb Haemost. 2002;88(5):865-870. https://www.ncbi.nlm.nih.gov/pubmed/?term=Suchkova+V.N.%2C+2002%3B88%3A865%E2%80%93870
Altland OD, Dalecki D, Suchkova VN. Low-intensity ultrasound increases endothelial cell nitric oxide syntase activity and nitric oxide synthesis. J Thromb Haemost. 2004;2:637-643. https://doi.org/10.1111/j.1538-7836.2004.00655.x
Hanawa K, Ito K, Aizawa K. Low-intensity pulsed ultrasound induces angiogenesis and ameliorates left ventricular dysfunction in a porcine model of chronic myocardial ischemia. PLoS One. 2014;(9):8:104-863. eCollection 2014 https://doi.org/10.1371/journal.pone.0104863.
Shindo T, Ito K, Ogata T. Low-intensity pulsed ultrasound enhances angiogenesis and ameliorates left ventricular dysfunction in a mouse model of acute myocardial infarction. Arterioscler Thromb Vasc Biol. 2016;(36):6:1220-1229. https://doi.org/10.1161/ATVBAHA.115.306477
Huang JIA, Shi YO, Li RL. Angiogenesis effect of therapeutic ultrasound on HUVECs through activation of the PI3K-Akt-eNOS signal pathway. Am J Transl Res. 2015;(7):6:1106-1115. http://www.ajtr.org/files/ajtr0004723.pdf
Cleicher N, Vidali A, Barad DH. Successful treatment of unreceptive thin endometrium. Fertil Steril. 2011;(95):6:2123-2137. https://doi.org/10.1093/humrep/des370
Jerman LF, Hey-Cunningham A. The role of the lymphatic system in endometriosis: a comprehensive review of the literature. J Biol Reprod. 2015;(92):3:64-66. https://doi.org/10.1095/biolreprod.114.124313
Koukourakis MI, Giatromanolaki A, Sivridis E. LYVE-1 immunohistochemical assessment of lymphangiogenesis in endometrial and lung cancer. J Clin Pathol. 2015;(58):2:202-206. https://doi.org/10.1136/jcp.2004.019174
Shurshalina AV. Chronic endometritis: current views on the problem. Consilium Medicum. 2011;6:36-39. Accessed August 28, 2017. (In Russ.)
Mityurina EV, Perminova SG, Demura TA, Gallyamova EM. Morpho functional state of the endometrium in stimulated cycles of in vitro fertilization program. Akusherstvo i ginekologiya. 2014;11:80-87. Accessed August 28, 2017. (In Russ.)

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