Objective. To evaluate the expression of TLR2, TLR3, TLR4, TLR7, TLR8, TLR9 and proteins of their signaling pathway TRIF, IRAK4 in different clinical forms of genital herpes (GH). Methods. 150 patients with GH were divided into 3 groups according to clinical forms of GH: group 1 (n=56) with first episode of non-primary infection; group 2 (n=53) with rarely recurrent GH and group 3 (n=41) with often recurrent GH. Results. Patients with a rarely recurrent GH have lower expression of TLR7 and IRAK4 compared with women with first episode of non-primary infection. Expression of TLR3 was lower in the group of frequently recurrent form of GH compared with the group with rarely recurrent GH. Moderate positive correlation between TLR3 and TRIF adapter molecule was observed. Pain syndrome and lymphadenopathy were associated with increased expression of TLR2 and TLR4 and decreased expression of TLR3 and TLR8. Strong positive correlation between chlamydial infection and the number of herpes exacerbations was found. Conclusion. Different clinical forms of GH are associated with different pattern of TLR expression.