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Evdokimov V.V.

NII urologii Minzdravsotsrazvitiia Rossii

Kharlamova L.A.

NII urologii Minzdravsotsrazvitiia Rossii

Aĭbiatov D.T.

NII urologii Minzdravsotsrazvitiia Rossii

Turovetskiĭ V.B.

Biologicheskiĭ fakul'tet MGU im. M.V. Lomonosova

Erokhin A.S.

VNII plemennogo dela, Moskva

The comparison of two methods for detection of human spermatozoa with damaged membrane

Authors:

Evdokimov V.V., Kharlamova L.A., Aĭbiatov D.T., Turovetskiĭ V.B., Erokhin A.S.

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Journal: Russian Journal of Human Reproduction. 2012;(3): 68‑71

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Table of contents

THE COMPARISON OF TWO METHODS FOR DETECTION OF HUMAN SPERMATOZOA WITH DAMAGED MEMBRANE
THE COMPARISON OF TWO METHODS FOR DETECTION OF HUMAN SPERMATOZOA WITH DAMAGED MEMBRANE

To cite this article:

Evdokimov VV, Evdokimov VV, Kharlamova LA, et al. . The comparison of two methods for detection of human spermatozoa with damaged membrane. Russian Journal of Human Reproduction. 2012;(3):68‑71. (In Russ.)

Подписка 2025-2 (Russian Journal of Human Reproduction)
 
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The trend of recent years reveals marked reduction of fertility parameters of the ejaculate. An important criterion for fertility is the number of viable sperm membrane which is impermeable to vital dyes. In this paper we used two dyes - eosin and propidium iodide, which penetrate through the damaged cell membrane. Staining of native ejaculate gave similar results in study and control groups. Incubation of sperm within 24 hours showed a higher percentage of nonviable cells identified with the help of propidium iodide. The content of nonviable sperm after cryoconservation was also higher. Sperm motility is significantly decreased after incubation.

Keywords:

sperm viability
sperm viability
sperm motilit
sperm motilit

Authors:

Evdokimov V.V.

NII urologii Minzdravsotsrazvitiia Rossii

Kharlamova L.A.

NII urologii Minzdravsotsrazvitiia Rossii

Aĭbiatov D.T.

NII urologii Minzdravsotsrazvitiia Rossii

Turovetskiĭ V.B.

Biologicheskiĭ fakul'tet MGU im. M.V. Lomonosova

Erokhin A.S.

VNII plemennogo dela, Moskva

List of references:

  1. Abubakirov A.P. Povrezhdenie DNK spermatozoidov i muzhskoe besplodie. Urologiya 2009; 3: 86-90.
  2. Vorob'eva O.L., Voskresenskaya L.V., Odintsov L.A., Filatov M.V. Muzhskoe besplodie i narushenie strukturnoi organizatsii khromatina spermatozoidov. Sushchestvuet li svyaz'? Probl reprod 2005; 6: 56-62.
  3. Dolgov V.V., Lugovskaya S.A. i dr. Laboratornaya diagnostika muzhskogo besplodiya. M 2006; 144.
  4. Komarova M.V. Kharakteristika eyakulyata v otsenke reproduktivnykh vozmozhnostei cheloveka: Avtoref. dis. ... kand. biol. nauk. Ufa 2000; 19.
  5. Agarwel A., Said T. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update 2003; 9: 331-345.
  6. Darzynkiewicz Z., Juan G. et al. Cytometry in Cell Necrobiology: Analysis of APOPTOSIS and Accidental Cell Death (Necrosis). Cytometry 1997; 27: 1-20.
  7. Oehniger S.C., Kruger T.F. Male infertility diagnosis and treatment. Informa Healthcare. London 2007.
  8. Oliva R. Protamines and male infertility. Human Reprod Update 2006; 12: 417-435.
  9. Sergerie M. et al. Sperm DNA fragmention: threshold value in male fertility. Hum Reprod 2005; 20: 3446-3451.
  10. Sun J.G., Casper R.F. Detection of DNA fragmention in human sperm: correlation with fertilization in vitro. Biol Reprod 1997; 56: 602-607.
  11. Tarozzi N., Bizzaro D. et al. Clinical relevance of sperm DNA damage. Reprod Biomed Online 2007; 14: 746-757.
  12. Tesarik J., Mendoza C. Sperm nuclear DNA damage: update on the mechanism, diagnosis and treatment. Reprod Biomed Online 2006; 12: 715-721.
  13. Rukovodstvo VOZ po laboratornomu issledovaniyu eyakulyata cheloveka i vzaimodeistviya spermatozoidov s tservikal'noi sliz'yu. 4-e izd. M 2001.
  14. WHO laboratory manual fog the examination and processing of human semen. 5th ed. WHO (Geneva) 2010; 270.

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