Objective. To improve the results of reconstructive-plastic surgery with displaced skin-fascial flaps for closure of perineal wound defects. Subjects and methods. This investigation was conducted on 325 objects: 21 female cadavers; 304 women, including 102 people, whose perineal anatomical parameters were studied, and 202 patients who underwent perineal surgical interventions, followed by the closure of wound defects in a variety of ways. Results. There was a set of new morphometric data on the structure of the surface tissues of the female perineum. There was also evidence for the variability of anatomometric indicators of the female perineum according to the body type and weight. Based on anatomometric data, the investigators developed a model for the formation of a displaced skin-fascial flap from the posterior surface of the thigh during plasty of perineal wound defects. They determined the perineal anatomometric indicators that made it possible to calculate the sizes of displaced skin-fascial flaps and the likelihood of flap necrosis and ischemia in the postoperative period. High-risk areas were found over a length of sutured tissues; and a morphometric rationale was given for operative suturing techniques in general surgery and microsurgery in different parts of the wound. There was new clinical evidence for healing of the postoperative wounds formed using microsurgical precision techniques. The results of perineal plasty with general surgical methods, including those using displaced skin-fascial flaps, and with an individualized method based on the morphometric data obtained with microsurgical technology were comparatively studied. Conclusion. The investigation expands the understanding of the anatomy and morphometric characteristics of female perineal tissues and can be used to plan and individualize perineal plasty procedures, which can improve the long-term results of surgical treatment and the quality of life in patients.