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Goncharova Yu.O.

State Research Center for Applied Microbiology and Biotechnology

Khlopova K.V.

State Research Center for Applied Microbiology and Biotechnology

Evseeva V.V.

State Research Center for Applied Microbiology and Biotechnology

Kravchenko T.B.

State Research Center for Applied Microbiology and Biotechnology

Mironova R.I.

State Research Center for Applied Microbiology and Biotechnology

Bakhteeva I.V.

State Research Center for Applied Microbiology and Biotechnology

Solomentsev V.I.

State Research Center for Applied Microbiology and Biotechnology

Skryabin Yu.P.

State Research Center for Applied Microbiology and Biotechnology

Timofeev V.S.

State Research Center for Applied Microbiology and Biotechnology

MVLST-analysis of Bacillus anthracis strains of the evolutionary lineage B

Authors:

Goncharova Yu.O., Khlopova K.V., Evseeva V.V., Kravchenko T.B., Mironova R.I., Bakhteeva I.V., Solomentsev V.I., Skryabin Yu.P., Timofeev V.S.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2024;42(3): 12‑21

DOI: 10.17116/molgen20244203112

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To cite this article:

Goncharova YuO, Khlopova KV, Evseeva VV, et al. . MVLST-analysis of Bacillus anthracis strains of the evolutionary lineage B. Molecular Genetics, Microbiology and Virology. 2024;42(3):12‑21. (In Russ.)
https://doi.org/10.17116/molgen20244203112

Подписка 2025 (Molecular Genetics, Microbiology and Virology)
 
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INTRODUCTION

Anthrax is a particularly dangerous infection caused by the spore–forming bacterium Bacillus anthracis, the pathogenicity of which is provided by the plasmids pXO1 and pXO2, carrying the genes for toxin synthesis and capsules. The species B. anthracis is divided into three evolutionary lineages: A, B and C. The lineage A is represented by many canSNP groups and is distributed throughout the world. The lineage B is divided into three groups: B.Br.CNEVA (Central Europe), B.Br.Kruger (South Africa) and B.Br.001/002 (South Africa, Korea, USA, Eurasia, and most of the area is located in Russia).

THE AIM

Of this study was a comparative wgSNP-analysis and multi-virulence locus sequence typing (MVLST) of a representative sample of B. anthracis strains of the evolutionary lineage B.

MATERIAL AND METHODS

55 strains of B. anthracis lineage B which was isolated on the territory of the Russia, the USSR and other states, were studied. Whole genome sequencing was performed on the DNBSEQ-G400 platform for the strains from SRCAMB. Genomes assembly, multiple alignment, and phylogenetic analysis were performed in UGENE, MEGA 7.0, and PHYLOViZ 2.0. MVLST-analysis was performed using MVLSTpXO1 and MVLSTpXO2 schemes. Identification of core SNPs and visualization of the phylogenetic tree for wgSNP-analysis was carried in Snippy v. 4.6.0 and SplitsTree4 v. 4.19.2.

RESULTS

MVLST analysis revealed previously described and new sequence types (alleles of individual genes) and genotypes (combinations of alleles). We found genetic markers in the genes of the main pathogenicity factors of B. anthracis, allowing to establish the phylogenetic position and probable region of origin of the strain. The genetic relationship of the North Eurasian and Siberian strains of the B.Br.001/002 group with the Eastern European cluster of strains of the B.Br.CNEVA group is shown.

CONCLUSION

The description of the phylogeographic structure of the evolutionary lineage B B. anthracis revealed the genetic relationship of the clusters of strains of the canSNP groups B.Br.001/002 and B.Br.CNEVA and the evolutionary relationship of these groups with the hypothetical ancestral genotype of B. anthracis lineage B, which looks intriguing.

Keywords:

Bacillus anthracis
anthrax
genotyping
MVLST
pathogenicity factors

Authors:

Goncharova Yu.O.

State Research Center for Applied Microbiology and Biotechnology

Khlopova K.V.

State Research Center for Applied Microbiology and Biotechnology

Evseeva V.V.

State Research Center for Applied Microbiology and Biotechnology

Kravchenko T.B.

State Research Center for Applied Microbiology and Biotechnology

Mironova R.I.

State Research Center for Applied Microbiology and Biotechnology

Bakhteeva I.V.

State Research Center for Applied Microbiology and Biotechnology

Solomentsev V.I.

State Research Center for Applied Microbiology and Biotechnology

Skryabin Yu.P.

State Research Center for Applied Microbiology and Biotechnology

Timofeev V.S.

State Research Center for Applied Microbiology and Biotechnology

Received:

23.04.2024

Accepted:

04.06.2024

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