Enigmatic mycoplasma colonies: the analisis by scanning and transmission electronic microscopy

Rakovskaya I.V.; Andreevskaya S.G.; Barkhatova O.I.; Levina G.A.; Gorina L.G.; Zhukhovitsky V.G.

doi:https://doi.org/10.17116/molgen20213903110

Rakovskaya I.V.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia

Andreevskaya S.G.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia

Barkhatova O.I.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia

Levina G.A.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia

Gorina L.G.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia

Zhukhovitsky V.G.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia;
Sechenov the First Moscow State Medical University of the Ministry of Public Health

Enigmatic mycoplasma colonies: the analisis by scanning and transmission electronic microscopy

Authors:

Rakovskaya I.V., Andreevskaya S.G., Barkhatova O.I., Levina G.A., Gorina L.G., Zhukhovitsky V.G.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2021;39(3): 10‑17

DOI: 10.17116/molgen20213903110

Read: 1898 times

Download PDF (RU)

Contact the author

Table of contents

To cite this article:

Rakovskaya IV, Andreevskaya SG, Barkhatova OI, Levina GA, Gorina LG, Zhukhovitsky VG. Enigmatic mycoplasma colonies: the analisis by scanning and transmission electronic microscopy. Molecular Genetics, Microbiology and Virology. 2021;39(3):10‑17. (In Russ.)
https://doi.org/10.17116/molgen20213903110

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)

Использование инфографики авторами научных работ

Close metadata

MATERIAL AND METHODS

The investigation was carried by scanning and transmission electronic microscopy.

RESULTS

It was discovered that mycoplasma populations are heterogeneous at first. MC of all species are differing radically from typical CC. MC have spiral form with dense centre and blades, composed by rod — shape cells. Colonies are arranged by almost parallel rows of cells with contracted closely side by side. The ultra structure of MC cells is unusual. The cells of different species have the longitudinal fissure on the cell surface, which dilates at the one end or splits as hand. The cell and MC of different mycoplasma species have the same organization. The question about the origin of these forms and their role in the species conservation are discussing.

CONCLUSION

Our work showed that populations of mycoplasma cells are heterogeneous. Morphology and ultra structural organization MC and their cells are unique, what was shown for the first time by scanning and transmission microscopy.

Keywords:

mycoplasma

microcolonies

electronic microscopy

population heterogeneity

Authors:

Rakovskaya I.V.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia

Andreevskaya S.G.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia

Barkhatova O.I.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia

Levina G.A.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia

Gorina L.G.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia

Zhukhovitsky V.G.

N.F. Gamaleya Federal research Center for Epidemiology and Microbiology of the Ministry of Public Health of Russia;
Sechenov the First Moscow State Medical University of the Ministry of Public Health

Received:

02.02.2021

Accepted:

20.02.2021

Close metadata

References:

Timakov VD, Kagan GY. L-forms of bacteria and the family of Mycoplasmatacea in patology. M.: Medicine; 1973. (In Russ.).
Konstantinova ND, Rakovskaya IV. Submicroscopical organization of several mycoplasma spaces. GMEI. 1980;4:28-30. (In Russ.).
Razin S, Hayflck L. Highlights of mycoplasma research — an historical perspective. Biologicale. 2010;38:183-190. https://doi.org/10.1016/j.biologicals.2009.11.008
Levina GA, Barkhatova OI, Gorina LG, Goncharova SA, Gamova NA, Rakovskaya IV. The atypical forms of mycoplasmas persisting in the organism of mycoplasma infected persons. GMEI. 2012;4:104-109. (In Russ.).
Ermolaeva SA, Rakovskaya IV, Miller GG, Sysolyatina EV, Mukhachev AY, Vasiliev MM, et al. Nonthermal plasma affects viability and morphology of Mycoplasma hominis and Acholeplasma laidlawii. J Appl Microbiol. 2014;116(5):1129-1136. Epub 2014 Feb 11. https://doi.org/10.1111/jam.12445
Rakovskaya IV, Ermolaeva SA, Levina GA, Barkhatova OI, Mukhachev AY, Andreevskaya SG, et al. Microcolonies: a novel morphological form of pathogenic Mycoplasma spp. J Medical Microbiol. 2019;68(12):535-559. https://doi.org/10.1099/jmm.0.001081
Levina GA, Barkhatova OI, Rakovskaya IV, Gribova TN, Butenko IO, Pobeguts OV, et al. Stress causes the formation of a morphologically new type of Mycoplasma hominis. FEBS OPEN BIO. 2018;8(suppl 1):444. https://doi.org/10.1002/2211-5463.12453
Proctor RA, Christof von Eiff, Kahl BC, Becker K, McNamara P, Hermann M, et al. Small colony variants: a pathogenic form of bacteria that facilities persistent and recurrent infection. Nature reviews Microbiol. 2006;4:295-305. https://doi.org/10.1038/nrmicro1384
Melter O, Radojevic B. Small colony variants of Staphylococcus aureus — review. Folia Microbiol. 2010;55(6):548-58. https://doi.org/10.1007/s12223-010-0089-3
Davis KM, Isberg RR. Defining heterogeneity via single cell approachers. Bioessays. 2016;38(8):782-790. https://doi.org/10.1002/bies.201500121
Maharjan RP, Ferenci T, Reeves PR, Li Yang, Lui Bin, Wang L. The multiplicity of divergence mechanisms in a single evolution population. Genom Biol. 2012;13(6):41. PM CID: PMC 3446 313. https://doi.org/10.1186/gb-2012-13-6-r41
Dybvig K, Simecka LW, Watson YL, Cassell GH. High frequency variations in Mycoplasma pulmonis colony size. J Bacteriol. 1989;171:5156-5168. https://doi.org/10.1128/jb.171.9.5165-5168.1989
Rosengarten R, Kirchhoff H. Growth morphology of Mycoplasma mobile 165 K on solid surfaces: reproduction, aggregation and microcolony formation. Curr Microbiol. 1989;18:15-22. https://doi.org/10.1007/BF01568824
Yang L, Lelsbak L, Marvig R, Damriaer S, Workman C, Rau M, et al. Evolutionary dynamics of bacteria in a human host environment. Proc Nat Acad Sci. USA. 2011;108(18):7481-7486. https://doi.org/10.1073/pnas.1018249108
Avraham R, Haseley N, Brown D, Penaranda C, Jijon H, Trombetta J, et al. Patogen cell-to-cell variability drivers heterogeneity in host immune response. Cell. 2015;162:1309-1321. https://doi.org/10.1016/j.cell.2015.08.027
Borchsenius SN, Chernova OA, Chernov BM, Vyshnyakov IE. Mycoplasmas in biology and medicine in the beginning of XXI century. 2016;39-54, 132-142. (In Russ.).
Voelker LL, Dybvig K. Gene transfer in Mycoplasma arthritidis: transformation, conjugal transfer on Tn 916, and evidence for a restriction system recognition AGCT. J Bacteriol. 1996;178:6078-6081. https://doi.org/10.1128/jb.178.20.6078-6081.1996
Hutchison CA, Petterson SN, Gill SR. Global transposan multigenesis and a minimal Mycoplasma genome. Science. 1999;286(5447):2165-2169:2000;69(3). https://doi.org/10.1126/Science.286.5447.2165
Oleskin AV, Botvinko IV, Tsalkelova EA. Colonial organization and intracellular communication. Microbiology. 2000;69(3):310-324. (In Russ.).