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Plyuta V.A.

Institute of Molecular Genetics of the National Research Center «Kurchatov Institute»

Sidorova D.E.

Institute of Molecular Genetics of the National Research Center «Kurchatov Institute»

Zavilgelsky G.B.

State Research Institute of Genetics and Selection of Industrial Microorganisms of the National Research Center «Kurchatov Institute»

Kotova V.Yu.

State Research Institute of Genetics and Selection of Industrial Microorganisms of the National Research Center «Kurchatov Institute»

Khmel I.A.

Institute of Molecular Genetics of the National Research Center «Kurchatov Institute»

The effect of volatile organic compounds synthesized by bacteria on the expression from promoters of zntA, copA, and arsR genes induced in response to the action of copper, zinc and arsenic

Authors:

Plyuta V.A., Sidorova D.E., Zavilgelsky G.B., Kotova V.Yu., Khmel I.A.

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Journal: Molecular Genetics, Microbiology and Virology. 2020;38(3): 128‑135

DOI: 10.17116/molgen202038031128

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To cite this article:

Plyuta VA, Sidorova DE, Zavilgelsky GB, Kotova VYu, Khmel IA. The effect of volatile organic compounds synthesized by bacteria on the expression from promoters of zntA, copA, and arsR genes induced in response to the action of copper, zinc and arsenic. Molecular Genetics, Microbiology and Virology. 2020;38(3):128‑135. (In Russ.)
https://doi.org/10.17116/molgen202038031128

 
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INTRODUCTION

Heavy metals and arsenic, getting into the environment, cause its pollution, negatively affect human health. Volatile organic compounds (VOCs) synthesized by bacteria are able to modulate the growth and metabolism of pro- and eukaryotes, function as signals for distance communication between organisms, and influence the action of various stress agents.

MATERIAL AND METHODS

The effect of sulfur-containing dimethyl disulfide (DMDS) and ketones (2-undecanone, 2-nonanone, 2-heptanone, 2-pentanone and 2-propanone) on the expression from promoters of copA, zntA and arsR genes (PcopA, PzntA and ParsR) responding to stresses caused by the action of heavy metal ions and arsenic was studied. lux-Biosensors containing inducible promoters of the listed genes and the lux-operon as reporter were used. The degree of expression from the corresponding promoters was determined by the intensity of bioluminescence.

RESULTS

The action of 2-undecanone, 2-nonanone, 2-heptanone, and DMDS led to decreased expression of lux-operon, which is controlled by PcopA and ParsR. Pretreatment of biosensors with these VOCs caused a decrease in the induction of expression with PzntA and ParsR under the action of ZnSO4 and NaAsO2, respectively; in the presence of CuSO4, this effect was expressed only for 2-undecanone. Under the action of 2-pentanone there was an increase in expression of the lux-reporter from PcopA and PzntA. Pretreatment with 2-pentanone led to an increase of the expression of the lux-reporter from PzntA by 80%, compared with the action of ZnSO4 without 2-pentanone.

CONCLUSION

The effect of VOCs on the expression from promoters of genes that respond to stresses caused by copper, zinc, and arsenic is multidirectional and depends on the structure of VOCs.

Keywords:

volatile organic compounds
heavy metals
arsenic
lux-biosensors
copA
zntA
arsR genes

Authors:

Plyuta V.A.

Institute of Molecular Genetics of the National Research Center «Kurchatov Institute»

Sidorova D.E.

Institute of Molecular Genetics of the National Research Center «Kurchatov Institute»

Zavilgelsky G.B.

State Research Institute of Genetics and Selection of Industrial Microorganisms of the National Research Center «Kurchatov Institute»

Kotova V.Yu.

State Research Institute of Genetics and Selection of Industrial Microorganisms of the National Research Center «Kurchatov Institute»

Khmel I.A.

Institute of Molecular Genetics of the National Research Center «Kurchatov Institute»

Received:

13.09.2019

Accepted:

13.11.2019

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