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Smirnov G.B.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

Bodoev I.N.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

Makarova A.P.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

Butusova T.B.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

Veselovsky V.A.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

Gulyaev A.S.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

Shitikov E.A.

Moscow State University of Medicine and Dentistry named after A.I. Evdokimov, City Clinical hospital named after S.I. Spasokukotskyi

Ilyina E.N.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

Comparative genomics of the Escherichia coli strains AB1157, AB2463, AB2494 and AB1885

Authors:

Smirnov G.B., Bodoev I.N., Makarova A.P., Butusova T.B., Veselovsky V.A., Gulyaev A.S., Shitikov E.A., Ilyina E.N.

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Journal: Molecular Genetics, Microbiology and Virology. 2019;37(3): 134‑139

DOI: 10.17116/molgen201937031134

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Table of contents

COMPARATIVE GENOMICS OF THE ESCHERICHIA COLI STRAINS AB1157, AB2463, AB2494 AND AB1885
COMPARATIVE GENOMICS OF THE ESCHERICHIA COLI STRAINS AB1157, AB2463, AB2494 AND AB1885

To cite this article:

Smirnov GB, Bodoev IN, Makarova AP, et al. . Comparative genomics of the Escherichia coli strains AB1157, AB2463, AB2494 and AB1885. Molecular Genetics, Microbiology and Virology. 2019;37(3):134‑139. (In Russ.)
https://doi.org/10.17116/molgen201937031134

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
 
МСФ на ЯМ
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Abstract:

Whole genome sequencing of the Escherichia coli strains AB1157 and its mutants AB1885 uvrB5, AB2463 recA13, and AB2494 lexA1 deficient in the DNA repair, recombination, and mutagenesis systems was performed. It was found that the genomes of AB2463 recA13 and AB2494 lexA1 strains differ from the genome of ancestral strain by several hundred mutations which arose most probably in the course of single treatment of AB1157 with N-methyl-N-nitro-N-nitrosoguanidine. At the same time the genome of the strain AB1885 obtained after treatment with nitrous acid contained ten times less differences from its parent strain. Mutations in the AB2463 recA13 and AB2494 lexA1 genomes were predominantly GC to AT transitions, distributed randomly along the genomes and their locations did not coincide in these strains. The input of spontaneous mutations in differences between the strains studied aroused during the period from their isolation to the sequence experiments seems to be insignificant. Earlier known genetic and phenotypic traits annotated on the molecular level.

Keywords:

Escherichia coli
recA
lexA
uvrB
N-methyl-N-nitro-N-nitrosoguanidine
nitrous acid
whole genome sequencing

Authors:

Smirnov G.B.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

Bodoev I.N.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

Makarova A.P.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

Butusova T.B.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

Veselovsky V.A.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

Gulyaev A.S.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

Shitikov E.A.

Moscow State University of Medicine and Dentistry named after A.I. Evdokimov, City Clinical hospital named after S.I. Spasokukotskyi

Ilyina E.N.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency of the Russia, Moscow, Russia, 119435

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References:

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