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Zinovyeva M.V.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Mikluho-Maklaya 16/10, Moscow 117997, Russia

Sass A.V.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Mikluho-Maklaya 16/10, Moscow 117997, Russia

Vvedensky A.V.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Mikluho-Maklaya 16/10, Moscow 117997, Russia

Potapov V.K.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Mikluho-Maklaya 16/10, Moscow 117997, Russia

Nikolaev L.G.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Mikluho-Maklaya 16/10, Moscow 117997, Russia

Sverdlov E.D.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Mikluho-Maklaya 16/10, Moscow 117997, Russia;
Institute of Molecular Genetics RAS, Kurchatov sq. 2, Moscow 123182, Russia

Recombinant histones as an instrument for delivery of nucleic acids into eukaryotic cells

Authors:

Zinovyeva M.V., Sass A.V., Vvedensky A.V., Potapov V.K., Nikolaev L.G., Sverdlov E.D.

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Journal: Molecular Genetics, Microbiology and Virology. 2018;36(3): 30‑37

DOI: 10.17116/molgen20183603130

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To cite this article:

Zinovyeva MV, Sass AV, Vvedensky AV, et al. . Recombinant histones as an instrument for delivery of nucleic acids into eukaryotic cells. Molecular Genetics, Microbiology and Virology. 2018;36(3):30‑37. (In Russ.)
https://doi.org/10.17116/molgen20183603130

 
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Naturally occurring positively charged proteins can be promising carriers for nucleic acids transport in gene therapy. The most attractive alternative among them are histones. In this work we describe expression and purification of recombinant human histones H2A and H2B and of chimeric protein of histone H2A and HIV-1 TAT fragment (TAT-peptide). The proposed purification method allows for significant decrease of bacterial endotoxins content in the final histone preparation which makes possible to use these proteins in the in vivo experiments. The transfection ability of plasmid DNA complexes with core histones H2A and H2B and the chimeric histone was demonstrated. With the use of histones H2A and H2B a highly specific transfection of human HT1080 cell line was detected, whereas transfection with complexes of DNA with chimeric H2A-TAT protein was observed for many cell lines.

Keywords:

non-viral delivery of nucleic acids into cell
non-viral delivery of nucleic acids into cell
recombinant histones purification
recombinant histones purification
TAT-peptide
TAT-peptide
endotoxin
endotoxin

Authors:

Zinovyeva M.V.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Mikluho-Maklaya 16/10, Moscow 117997, Russia

Sass A.V.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Mikluho-Maklaya 16/10, Moscow 117997, Russia

Vvedensky A.V.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Mikluho-Maklaya 16/10, Moscow 117997, Russia

Potapov V.K.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Mikluho-Maklaya 16/10, Moscow 117997, Russia

Nikolaev L.G.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Mikluho-Maklaya 16/10, Moscow 117997, Russia

Sverdlov E.D.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Mikluho-Maklaya 16/10, Moscow 117997, Russia;
Institute of Molecular Genetics RAS, Kurchatov sq. 2, Moscow 123182, Russia

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