Abstract Despite significant progress in laboratory tests standardization, defining reference ranges (RR) remains a relevant problem. The aim of this study is to verify RR of cobas test systems for 81 analytes according to the international CLSI/IFCC standards. Material and methods. This study included 40 healthy volunteers: 20 men and 20 women aged from 20 to 49 years, selected after the survey from residents of St. Petersburg. Sampling biomaterial from all volunteers was carried out after an overnight fast in compliance with pre-analytical procedures. Specimens were prepared from venous blood serum, which was stored after sampling biomaterial at –70 °C. Target biomarkers included both standardized (blood lipids, electrolytes, enzymes, etc.) and harmonized (inflammation factors, hormones, vitamins, etc.) analytes. The tests were performed on the Roche cobas 8000 analytical system consisting of biochemical modules c 702/c 502 and immunochemical module e 602. Results. The results obtained in this study allow us to distinguish three groups of analytes: 1) RR of which are verified for both groups (men and women) — 70 analytes; 2) RR of which are verified for one of the groups (either men or women) — 7 analytes; 3) RR are not verified — 4 analytes. For most of the analytes of the last two groups regional differences were previously identified during establishing RR, or the expected values corresponded to international points of clinical decision making. Conclusions. 77 analytes were verified in this study according to the international CLI/IFCC protocols for RR defining and verification in clinical chemistry, — and that allows them to be used in a particular population routine laboratory testing. The remaining 4 RR are objects of further researches, as their deviations may be associated with geographical, population and other factors.