Pre-analytical phase of laboratory tests plays an important role for the correctness and admissibility of results in a trend of centralization of laboratory tests. The assessment of the analytes stability in different storage conditions allow to define sample storage parameters in which meaningful concentration changes of a analytes do not appear. The objective was the assessment of human’s blood analytes stability in different temperature conditions and to determine an impact of cell components on analyte resistant with time. Material and methods. Sixty blood samples were selected for the research and divided into groups: samples in the first group were without cell components and in second were with them. The samples from both groups were placed into established temperature conditions (+4 °С, +23 °С и +30 °С). The measuring of ALT, AST, total bilirubin, glucose, potassium, creatinine, CK, LDH, urea, sodium, total protein, triglycerides, alkaline phosphatase, chlorine, cholesterol was done after 4, 24, 48 and 72 hours in first group and after 4, 8, 24, 48 hours in second group. Results. Clinically meaningful changes of glucose and potassium levels were determined in samples of patients from second group. The glucose level clinically meaningful changed at +4 °С in 48 hours, at +23 °С in 48 hours (24 hours in case RCV = 80%), at +30 °С in 24 and 48 hours. The potassium level clinically meaningful changed at +4 °С in 24 and 48 hours, at +23 °С and +30 °С in 48 hours. Conclusion. The results of the research demonstrate the importance of compliance with the temperature conditions and the sample storage time and it allows to determine optimal storage parameters for samples.