Current trends in modern medical science are associated with the search for new non-invasive methods that allow performing diagnostic examinations of biotissues and monitoring of changes occurring in the course of therapy without causing additional skin defects, which inevitably occurs after biopsy. 3D-optical coherence tomography (3D-OCT), which is an optical analogue of excisional skin biopsy, is a non-invasive high-resolution (10—20 microns) method. Low-intensity near-infrared light with a power of up to 1.5 mW is used as probing radiation. This method allows to verify main pathomorphological processes in the epidermis, in the area of the dermo-epidermal connection and in the papillary, and in some locations — also in the reticular part of dermis; helps in the differential diagnosis, not distorting the architectonics of tissues, allows to visualize the morphology of the same skin area in dynamics. The study describes the identification and comparison of optical layers on OCT images and components of healthy skin of different anatomical localization, demonstrates the features of the structure of thick and thin skin from different anatomical zones, provides the optical images of skin appendages (sebaceous, sweat glands, hair and nails). The proposed method is a new and promising one, it has the prerequisites for further development and wide implementation in practice.