Trichomonas vaginalis is a protozoan pathogen causing urogenital trichomoniasis, one of the most common sexually transmitted infections. Microscopy and culture are known to be the classical methods for the detection of T. vaginalis. However, the nucleic acid amplification techniques has recently become the increasingly popular tools for the diagnostics of this and other infections due to the possibility of detecting pathogens even if present in minimal amounts. The authors report the results of the elucidation of the detection limits of different methods used to identify T. vaginalis: microscopy, culture, PCR, and NASBA, in the laboratory diagnostics of urogenital trichomoniasis. The nucleic acid amplification techniques (PCR and NASBA with the use of AmpliSense reagent kits) were shown to possess the highest analytical sensitivity (the lowest detection limit) for the identification of T. vaginalis. The lowest sensitivity was documented for microscopic methods while sensitivity of the culture was found to be intermediate between that of microscopic and nucleic acid amplification techniques.