Possibility of DNA isolation from archived autopsy tissues for molecular genetic studies

Viazovskaia N.S.

FGUP Iuzhno-Ural'skiĭ institut biofiziki, Ozersk

Rusinova G.G.

FGUP Iuzhno-Ural'skiĭ institut biofiziki, Ozersk

Azizova T.V.

Southern Ural Biophysics Institute of the Federal Medical Biological Agency of Russia, Ozersk, Chelyabinsk region, Russia

Revina V.S.

FGUP Iuzhno-Ural'skiĭ institut biofiziki, Ozersk

Glazkova I.V.

FGUP Iuzhno-Ural'skiĭ institut biofiziki, Ozersk

Generozov E.V.

Sechenov First Moscow State Medical University of Healthcare Ministry of the Russia, Moscow, Russia, Federal Research Clinical Center for Physico-Chemical Medicine, FMBA of Russia, Moscow, Russia

Zakharzhevskaia N.B.

Laboratoriia molekuliarnoĭ genetiki cheloveka FGU NII FKhM Roszdrava, Moskva

Matiushkina D.S.

Laboratoriia molekuliarnoĭ genetiki cheloveka FGU NII FKhM Roszdrava, Moskva

Possibility of DNA isolation from archived autopsy tissues for molecular genetic studies

Authors:

Viazovskaia N.S., Rusinova G.G., Azizova T.V., Revina V.S., Glazkova I.V., Generozov E.V., Zakharzhevskaia N.B., Matiushkina D.S.

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Journal: Russian Journal of Archive of Pathology. 2014;76(2): 46‑47

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To cite this article:

Viazovskaia NS, Rusinova GG, Azizova TV, et al. . Possibility of DNA isolation from archived autopsy tissues for molecular genetic studies. Russian Journal of Archive of Pathology. 2014;76(2):46‑47. (In Russ.)

Подписка 2025 (Russian Journal of Archive of Pathology)

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Optimal conditions were defined for DNA isolation from formalin-fixed and paraffin-embedded (FFPE) archived autopsy lung tissue slices. The quality of DNA preparations isolated from the FFPE archived slices was assessed and their suitability for further molecular genetic analysis estimated. DNA isolated from the FFPE slices stored less and more than 10 years was suitable for molecular genetic studies in 100 and 66.7% of cases, respectively.

Keywords:

molecular genetic analysis

formalin-fixed and paraffin-embedded slices

archived tissue DNA

Authors:

Viazovskaia N.S.

FGUP Iuzhno-Ural'skiĭ institut biofiziki, Ozersk

Rusinova G.G.

FGUP Iuzhno-Ural'skiĭ institut biofiziki, Ozersk

Azizova T.V.

Southern Ural Biophysics Institute of the Federal Medical Biological Agency of Russia, Ozersk, Chelyabinsk region, Russia

Revina V.S.

FGUP Iuzhno-Ural'skiĭ institut biofiziki, Ozersk

Glazkova I.V.

FGUP Iuzhno-Ural'skiĭ institut biofiziki, Ozersk

Generozov E.V.

Sechenov First Moscow State Medical University of Healthcare Ministry of the Russia, Moscow, Russia, Federal Research Clinical Center for Physico-Chemical Medicine, FMBA of Russia, Moscow, Russia

Zakharzhevskaia N.B.

Laboratoriia molekuliarnoĭ genetiki cheloveka FGU NII FKhM Roszdrava, Moskva

Matiushkina D.S.

Laboratoriia molekuliarnoĭ genetiki cheloveka FGU NII FKhM Roszdrava, Moskva

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