A new method for determining active fibrinogen using thromboelastography

Galstyan G.M.

National Research Center for Hematology, Moscow, Russia

Polevodova O.A.

National Research Center for Hematology, Moscow, Russia

Berkovskiy A.L.

National Research Center for Hematology, Moscow, Russia

Sergeeva E.V.

National Research Center for Hematology, Moscow, Russia

Savchenko V.G.

National Research Center for Hematology, Moscow, Russia

A new method for determining active fibrinogen using thromboelastography

Authors:

Galstyan G.M., Polevodova O.A., Berkovskiy A.L., Sergeeva E.V., Savchenko V.G.

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Journal: Russian Journal of Anesthesiology and Reanimatology. 2018;(3): 86‑94

DOI: 10.17116/anaesthesiology201803186

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Galstyan GM, Polevodova OA, Berkovskiy AL, Sergeeva EV, Savchenko VG. A new method for determining active fibrinogen using thromboelastography. Russian Journal of Anesthesiology and Reanimatology. 2018;(3):86‑94. (In Russ.)
https://doi.org/10.17116/anaesthesiology201803186

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We are various methods to evaluate plasma fibrinogen level. Thromboelastography (TEG) allows estimating functional fibrinogen level by Functional Fibrinogen test (Haemonetics). The principle of the Functional Fibrinogen test is based on the inhibition of platelets. We propose the TEG-batroxobin test for valuation of fibrinogen level. In TEG-batroxobin test the platelets are suppressed by heparin, and the formation of fibrin clot is achieved by cleaving of fibrinopeptide A from fibrinogen. The aim of our study was to develop the TEG method for evaluation of active fibrinogen level in blood. Material and methods. Batroxobin was obtained from the poison of the rattlesnake Bothrops atrox. Two fractions with thrombin-like activity were isolated from batroxobin by chromatography. 30 blood samples were obtained from 17 patients. The fibrinogen concentration (Clauss method), functional fibrinogen levels (TEG, Functional Fibrinogen Reagent, Haemonetics) and active fibrinogen levels (TEG with batroxobin) were estimated. Results. The plasma concentrations of fibrinogen varied from 0.5 to 8.7 g/l. The values of MABATROX varied from 2.3 to 62.5 mm and MA in Functional Fibrinogen test varied from 7.6 to 54.5 mm. There were strong correlations between MABATROX and fibrinogen concentrations (r=0.83; p<0.001), between MABATROX and MA in Functional Fibrinogen test (r=0.88; p<0.001). The TEG with batroxobin reflected the concentrations of active fibrinogen in patients with hypo- and hyperfibrinogenemia. Conclusion. The TEG with batroxobin can be used for estimating of active fibrinogen levels in in critically ill patients.

Keywords:

thromboelastography; functional fibrinogen; active fibrinogen; batroxobin

Authors:

Galstyan G.M.

National Research Center for Hematology, Moscow, Russia

Polevodova O.A.

National Research Center for Hematology, Moscow, Russia

Berkovskiy A.L.

National Research Center for Hematology, Moscow, Russia

Sergeeva E.V.

National Research Center for Hematology, Moscow, Russia

Savchenko V.G.

National Research Center for Hematology, Moscow, Russia

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